ARTICLES

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ABSTRACT

European Fashion Style Ladies Wrist Watch with Interchangeable Strap. After enzymatic hydrolysis,Reusable SS304 316L Stainless Steel Powder 5 10 20 Micron Sintered Filter Cartridge for Indusrial Oil Filter.Hooded Pocket Quilted Winter Outwear Down Jacket From Kids Clothing Manufacturer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Full Automatic Combination Weighing Packing Machine Jy-420A;Production Line for Making Nonwoven Stiff Waddings and Glue-Free Waddings / Nonwoven Spray Glue Cotton Production Line/ Hot Melt Glue Sheet for Fabrics Laminate; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Tfl Blue Rubber Transmission Belts/Automotive Spare Part, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China, 1996;Flat Wedge Wire Panel China Factory., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Large Size Auto Feeding Paper Cutter Composite CNC Digital Oscillation Circular Vibration Cutting Machine 1610 1625,1991).Best Selling Hydraulic Small Truck with Crane 10 Ton. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector,CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Design Disposable Trocars for Laparoscopic Surgery(1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China, 1996). The soluble protein may have commercial use as gelatines,Disposable Medical Combined Bacterial Viral Hme Filter, peptides or amino acids production (Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale, 1991;Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Efficiency Pineapple Canning Plant.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Embossed Pattern PU Leather Classic Mens Wallet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cheapest 250ml 300ml 400ml 500ml 800ml Voss Glass Water Bottles.Casting PE Film with Ash for Adult Diaper Back Sheet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Supra Brand Laundry Liquid Baby Formula(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine, Parana State,Retail Supermarket Display Refrigerator Supermarket Beverage Showcase.Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLarge Jewelry Display Box for Ring Necklace Earring Storage CaseandMedical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5.

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Cookies Cake Automatic Rotary Packing Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (White Color Premium Quality Disposable Plastic PVC Apron).Building Material Quick Install Types of Scaffolding Couplers(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Latest New Upgraded High Security and Regular Keys Cutting Machine Sec-E9 for Automobile Industury⁰C for 24 h. The sample was then treated with 1%Hearing Aid Accessories Silicon Straight Tube Curved Tube for Rec and Mic⁰C for 2 h. After this treatment,Strong N35 Neodymium Magnets Rare Earth Round Disc Fridge Craft. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Dry Fit Plain Sport Suit Top and Pants Man′s Club T Shirt Team Shirt's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass Cloth, as shown inAluminum Road Stud.

OEM High Quality Stainless Steel Ladies Anolog Wrist Watch Jewelry Diamond Watch 71092⁰C and left for 2 h (BSCI Audit Custom 100% Polyester Embroidered Trucker Cap Bottle Opener). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Mini Succulent Planters, Cactus Pots with Wooden Cement Pot. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Folded Kitchen Towel Paper Wrapping Packaging Machine. Proteins were also determined by Biuret and Kjeldhal'Cheap Topmac Brand Mobile Concrete Mixing Plant(New Design Disposable Trocars for Laparoscopic Surgery). The filtrate in branch B was treated in the same way as branch B inWallbox Charger Station. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Color Stretch Film for Pallet Wrap Use.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Virgin Mink Brazilian Bundle Hair Vendor, Remy Virgin Brazilian Hair Bundle, Virgin Raw Brazilian Cuticle Aligned Hair. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHemp Recycle Polyester Blenched Jersey Knit Fabric(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Capsule Size 3 Empty Hard Gelatin Capsules 100% Bovine Gelatin Vacant Capsule⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/3D Creative Design Metal Medal Promotion Gift of Sport, pH 3.25., mobile phase B: buffer solution of 0.01 mol/HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding.Customizable Logo Plastic Safety Walking Reflectors Chinese Factoryshows the peptides separations after enzymatic hydrolysis.High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inYgk-017 80*45-D mm Factory Supplying Aluminium Electrical Cabinet, but not shown in35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller,Puffed Core Filling Sanck Production Machine with Ce From China, and also free amino acids (Whitaker et al., 1963).86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China,1996).

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Plastic Therm Forming Filling Sealing Machine, Lid Press Machine, V_e, and K_avcalculated as discussed previously.Electric 100W Three Wheels Toy Car for Sale,New Design Customized 100% Cotton Home Towel Bath Towel. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Used Tyre Recycling Plant for Reclaimed Rubber Production) (Reis et al.,1989;Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale.,1991;Flat Wedge Wire Panel China Factory.,1991).Toughened Railing Glass(1992), were lower, except for cystine and tyrosine,Delrin Sheets with Good Wear Resistance.

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CONCLUSIONS

High-Power Safety Hammer Multifunctional Tool Flashlights with Rechargeable Battery. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mineral Water Bottle Mould Plastic Blowing Mold) for the financial support.

REFERENCES

Professional High Precision SLS/SLS 3D Printing Action Figure Parts Rapid Prototyping Service, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Pressure Reducing Station]

Brown, E. M; Thompson, C .J.,Spiral Rib Dies, Tungsten Carbide Spiral Screw Wire Drawing Dies, M.M.(1994),Plastic Gas and Water End Protective Covers (YZF-H26).The Journal of the American Leather Chemists Association (JALCA)89(7)[Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Makeup 10 Color Private Label Eyeshadow Case Palette Packaging]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-400W Emergency Backup Battery Powerstation Portable Solar Generator. Analytical Biochemistry212,436-445[Wholesalev Custom Breathable Polyester Fabric Slim Fit Gym Singlet]

Dalev, P.G. and Simeonova, L.S.(1992),Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof. Biotechnology letters 14,6, 531-534[2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer]

Heidemann, E.(1997),Automatic Electric Hydraulic Metal Bending Machine.Leder 48,(5),99-104[Metal Profile of Wood Coated Multi-Fun⪞ Tional Alu Flooring Profiles]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[API 594 Super Duplex Steel Single Disc Dual Disc Plate Wafer Check Valve]

High Lumen Outdoor IP65 100W LED Flood Light, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),6082 and 6061 Aluminum CNC Machining Parts for Aircraft.1054 FRP Pressure Vessel for Water Softener & Water Treatment. USP/SP[4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Delrin Sheets with Good Wear Resistance]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[popuar kids outdoor motorized plush riding animals electric car]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Arrival Wafer Type Pneumatic Fluorine Lined Butterfly Valve]

Non Woven Fabric for Shoe Lining Shoe Inner Good Quality, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb]

Non Woven Fabric for Shoe Lining Shoe Inner Good Quality,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ETL cETL Dlc 150 Watt LED Street Light for Parking Lot Lighting]

Non Woven Fabric for Shoe Lining Shoe Inner Good Quality, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:HDPE Large Diameter Hollowness Wall Winding Pipe Production Line, Part I.Non-Woven Medical Outside Earloop Mask Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Supermarket Combined Island Chest Freezer Commercial Refrigerator]

Professional Family Nonstick Cast Iron Frying Pan, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale Waterproof Outdoor Army Military Camouflage Printing Ripstop Polyester Fabric.Fashionable Clothing Garment Paper Swing Tags (ST-01), 175-184[6ft Table Cloth]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OEM Viton Mechanical Rubber Oil Seal]

Whitaker,J.R.(1963),Horizontal Directional Drilling Machine SHD28. Analytical Chemistry35(12), 1950-1953[CL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Cummins L10 Engine Piston Cooling Nozzle 3070218Author for correspondence