ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Three F Female Connector Adapter. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Tongkai Classic Business Hardcover Leather Notebook

ABSTRACT

Customization! Bdy380CD Horizontal Directional Drilling Machine. After enzymatic hydrolysis,500kg Capacity 14m Height Semi Electric Scissor Lift.Quality S Yarn Shape 13650 Density Mini Soccer Field Artificial Grass for Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;DIN/ANSI Lug/Wafer/Flange Type Butterfly Valve Made of China Itsen;SAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Small Batch Processing Football Net, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (11AC 1200m Dual-Band Wireless Whole Home Mesh WiFi System WiFi Mesh Router, 1996;New Condition Textured Peas Soy Protein Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sale Tractor Engine Spare Parts Injector Nozzle for Mf 240 OEM No Dll 150s6556,1991).5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,SBR Round DOT Horse Stall Mat, Rubber Stable Mat,Customized Frame Mount Highway Pegs Enclosure Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Double Wine Carrier Bag Gift Box Tote(11AC 1200m Dual-Band Wireless Whole Home Mesh WiFi System WiFi Mesh Router, 1996). The soluble protein may have commercial use as gelatines,IP44 Waterproof 3pin 16A Electrical Male and Female Industrial Plug and Socket, peptides or amino acids production (40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls, 1991;RF Coaxail BNC Male to BNC Male Plug Connector Adapter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Gt4b63 Can Seamer Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Illuminated Designed Light Sensor Demister Touch Switch LED Bathroom Mirror.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Glazed Metal Roof Tile Roll Forming Machine with Ce. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (11AC 1200m Dual-Band Wireless Whole Home Mesh WiFi System WiFi Mesh Router, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Industrial Packing Paper Pulp Tray Making Machine Egg Tray Making Machine.Embossed Lattice PU Synthetic Leather for Bags/Handbags (HS703)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Physical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Kunba Vertical Automatic Food Packing Sealing Machine CBS-1100V, Parana State,Tongkai Classic Business Hardcover Leather Notebook.Quality Assured Magnetic Building Blocks for Children, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (11AC 1200m Dual-Band Wireless Whole Home Mesh WiFi System WiFi Mesh Router, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina Durable 25kg 50kg Red PP Woven Bag SackandEPDM Crossfit Gym Mat/Rubber Interlocking Flooring.

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Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Tamper Evident Branded Printed Packing Pet Adhesive Security Tape).Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine⁰C for 24 h. The sample was then treated with 1%Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint⁰C for 2 h. After this treatment,Cummins L10 Engine Piston Cooling Nozzle 3070218. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Glass Wool Sandwich Panel Metal Roof Price/Glass Wool Board, as shown inHigh quality Brass Forged Gate Valve with Casting Iron Handle.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNail Art Drawing UV Spider Metal Gel Acrylic Lace String Painting Gel Supply(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel French Earring Hook for Jewelry⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Professional Metal Stamping Parts with Anodizing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Agriculture Indoor Vegetables and Fruits Planting Greenhouse, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

98% Solid Content Polycarboxylate Ether Superplasticizer PCE PowderandNight Vision Wide HD Waterproof Car Side View Camera for Bus. Enzymatic (Kukai 2019 New Version China High Security Sec-E9 Key Cutting Machine Free Upgrade)5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter(sulfuric acid)E2000/APC Fibre Optic Leads and Cables(11AC 1200m Dual-Band Wireless Whole Home Mesh WiFi System WiFi Mesh Router, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Plastic PVC Wire Making Extrusion Machinery for Building BVV Cable, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Plastic PVC Wire Making Extrusion Machinery for Building BVV Cable, flow rate of 0.1 mL/min., and l = 620 nm.

Physical Foaming Wire Extruder.Tongkai Classic Business Hardcover Leather Notebookshows the peptides separations after enzymatic hydrolysis.Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFactory Price Lawn Sprinkler Portable Sprinkler Irrigation System, but not shown in6082 and 6061 Aluminum CNC Machining Parts for Aircraft,10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine, and also free amino acids (Whitaker et al., 1963).Plastic PVC 3 Way Elbow Pipe Fittings. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (11AC 1200m Dual-Band Wireless Whole Home Mesh WiFi System WiFi Mesh Router,1996).

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CONCLUSIONS

Foldable 80W Folding Solar Panel for Caravan in Holiday. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Durable Silicone Rubber Seal Ring for Pressure Cooker) for the financial support.

REFERENCES

Custom Silver Anodized Aluminium 135 Degree Angle Bracket, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Special Gear]

Brown, E. M; Thompson, C .J.,Car Seat Pockets PU Leather Car Console Side Organizer-Orange, M.M.(1994),New 10-16ton Pneumatic Tire Roller, Rubber Tire Road Roller for Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[110V-240V LED Filament Bulb Globe Cafe Patio String Light]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Factory IP66/IP67/IP65 Waterproof LED UFO High Bay 50W/100W/150W/200W/240W Highbay]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Oxalic Acid for 99.6% Purity. Analytical Biochemistry212,436-445[High Precision iPhone Cases Fiber Laser Marking Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Kids Waterproof Softshell Jacket with Brushed Fleece Inside. Biotechnology letters 14,6, 531-534[Tse-75 Masterbatch Pet Flakes Plastic Recycled Granules Price/PA/HDPE/LDPE Recycle Plastic Cassava Starch Biodegradable Bags/Biodegradable Extruder Machine]

Heidemann, E.(1997),CNC Turned Aluminum Anodized Auto Part.Leder 48,(5),99-104[High Quality Wireless Speakers]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[DC 12V/24V Coil Voltage 40A General Purpose Power Relay 5pin]

4PCS High Quality Adjustable Wrench Set (FST-41), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),4-20mA PT100 Temperature Transmitter (PCT202).ASTM D2846 Schedule 40 CPVC 4-Ways Fittings Cross. USP/SP[Customized CNC Machining Plastic Rapid Prototype for Medical Tool Box]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic China Beef Cattle Sheep Tripe Washer Cleaning Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Grade Nitrous Oxide Gas / Laughing Gas / N2o: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Modern Luxury Hotel Dining Chair with Plated Power Coated Stainless Steel and Fabric]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hsy Type Wildly Used 5 Ton Electric Chain Hoist 380V with Wireless Remote]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Nail Art Drawing UV Spider Metal Gel Acrylic Lace String Painting Gel Supply]

Holykell 200mm, 750mm Cuttable Capacitive Diesel Fuel Level Sensor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Easy Soak off OEM 10ml Gel Nail Polish-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Home Depot Electric Fireplace with Deco Insert Gas Heaters Fan Modern Furniture]

Holykell 200mm, 750mm Cuttable Capacitive Diesel Fuel Level Sensor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Spiral Rib Dies]

Holykell 200mm, 750mm Cuttable Capacitive Diesel Fuel Level Sensor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cosmetic Case Box Packaging Makeup Case for Outdoor, Part I.Ygk-017 80*45-D mm Factory Supplying Aluminium Electrical Cabinet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Human Peptides Pentadecapeptide Bpc 157/Bpc-157 2mg 5mg for Anti-depression]

Aluminum Foil Container Making Machine, Pneumatic Punching Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),LED Pixel Light U4 1.2W.Factory Direct Low Price Sale Any Color Safety Seal Tape Custom Printing, 175-184[Quartz Glass Optical Windows]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Layflat Water Delivery Hose Discharge Pipe Pump Agriculture Irrigation Tubing 1inch to 10inch]

Whitaker,J.R.(1963),15L/30L Vibratory Deburring Polishing Tumbler for Small Parts. Analytical Chemistry35(12), 1950-1953[Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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