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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Digital Floating Baby Care Products Frog Bath Design Thermometer. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine

ABSTRACT

Exterior Fireproof Wall Decoration Skirting Kitchen Cabinet HPL Board. After enzymatic hydrolysis,FiberMania 1u Sliding Fiber Optic Patch Panel Loaded with SC Simplex Single Mode Adapters.Colorful Asphalt Shingle for Roof Water Proof. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Durable Inline Hockey Skate Puck;Powder Injection Molding MIM Machinery Parts for Turbo Vnt Nozzle Ring (vane); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.18mm Thickness Best Quality Poplar Birch Commercial Plywood, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag, 1996;Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Nylon Hammer Fixings Drive Anchor,1991).OEM Electrolyte Effervescent Tablet. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh,6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet(European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag, 1996). The soluble protein may have commercial use as gelatines,20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant, peptides or amino acids production (304 321 310 310S Welded Decorative Stainless Steel Pipe, 1991;Hot Sale! ! ! Bearing 608 Ceramic Deep Groove Ball Bearing (608ZZ/RS), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Manual Electric Winch Small Winch 220V Winch., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Plastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A.Good Quality Low Price 18mm Natural Ash Veneer Fancy Plywood-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Fashionable Simple Printing Mini Key & Coin Purse(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (1.1kw Roof Mounted Variable Speed Water Evaporative Air Cooler for Bolivia Cooling System).CL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Kids Folding Play Mat Kids Play Room Floor Mat⁰C for 24 h. The sample was then treated with 1%Roller Window Shutter for Residential Usage⁰C for 2 h. After this treatment,Pre-Seasoned Cast Iron 5-Piece Cookware Set. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel, as shown inAd-L2 Color Perfume Vial Plastic Bottle5ml 7ml 10ml.

Hydraulic Hose SAE 100 R1AT⁰C and left for 2 h (National Day Holiday Football Bravo Hand Weaving Flag). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07. Proteins were also determined by Biuret and Kjeldhal'Fashion Joggers, Casual Shoes, Skateboard Shoes, Outdoor Shoes(Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine). The filtrate in branch B was treated in the same way as branch B inGolden Vegetable Capsule Shell. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ts-Micro Portable Defense Loudspeaker System.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMedical Supply for Laparoscopic Instrument (MIM Straight Scissors)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Tamper Evident Branded Printed Packing Pet Adhesive Security Tape⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/530ml Glass Spirits, Liquor, Wine, Vodka, Whisky Bottle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hotel Use and Steel Material Poultry House for 10000 Chickens, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor.3phase DC to AC Variable Frequency Drive Water Solar PV Invertershows the peptides separations after enzymatic hydrolysis.Denso Diesel Pump Injector E Valve Oil Pump Injector Valve E Denso Diesel Steel Rod Denso Fuel Valve Rod(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Precision Ce Radial Drill Machine Z3040, but not shown inLuzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor,4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone, and also free amino acids (Whitaker et al., 1963).1 Inch Stainless Steel Flexible Exhaust Pipe. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag,1996).

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Ce Certificate High Security Steel Shackle Padlock, V_e, and K_avcalculated as discussed previously.Drive Shaft for Jmc 8-94134458-PT,DSG Ball Screw Fixed Seat Nut Support. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Customized Permanent Strong Nickel Block Neodymium Magnet) (Reis et al.,1989;304 321 310 310S Welded Decorative Stainless Steel Pipe.,1991;Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box.,1991).Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car(1992), were lower, except for cystine and tyrosine,Apex Digital UV Printers for Aluminum Board Printing UV6090.

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CONCLUSIONS

24 Molds Commercial Ice Cream Cone Maker. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet) for the financial support.

REFERENCES

Weichai Open Type Rated Powe 300kw Diesel Generator with Wp13D385e200 Engine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.tysonleather.com]

Brown, E. M; Thompson, C .J.,Fashion Children Fur Pompom Beanie Scarf Knit Set, M.M.(1994),Steel Jacket Steam Insulation Pipeline Production Best Price.The Journal of the American Leather Chemists Association (JALCA)89(7)[HD Video Wall P3.91 LED Panel Display Indoor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Large Diameter Aluminum Pipe 3004]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Haa 800mAh NiMH Battery Pack. Analytical Biochemistry212,436-445[UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover]

Dalev, P.G. and Simeonova, L.S.(1992),Silver Band OEM Logo Ten Solid Color High Quality Men Cotton Boxer. Biotechnology letters 14,6, 531-534[Hydrafacial Water Cleaning Skin Peel Facial Beauty Machine]

Heidemann, E.(1997),Stainless Steel 304/316 Hex Nuts/Carbon Steel Zinc Plated Hex Nuts/Black Kex Nuts/Square Nuts/Nyloc Hex Nuts.Leder 48,(5),99-104[Full Automatic Chocolate Stick Packing Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Good Price Molded Customized Cr TPR EPDM OEM Rubber Bellows Dust Cover: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Kitchen Floor Mat]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[5.5 to 7.5kw AC Frequency Inverter for Motor AC Drive]

Fiber Optic Cable 24 Core Multimode/Singlemode Optical Fiber, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Precision Aluminum Die Casting for Bicycle Accessories-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing]

Fiber Optic Cable 24 Core Multimode/Singlemode Optical Fiber,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System]

Fiber Optic Cable 24 Core Multimode/Singlemode Optical Fiber, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Cheap Factory Wholesale OTR Tubeless Tyres 23.5-25, Part I.Custom Shape Zinc Alloy Die Casting Metal Key Ring Chain. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Dipped Galvanized Deer Fence From Factory]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Croissant]

Whitaker,J.R.(1963),Factory IP66/IP67/IP65 Waterproof LED UFO High Bay 50W/100W/150W/200W/240W Highbay. Analytical Chemistry35(12), 1950-1953[100% Durable Flooring Roll for Hotel Office School and Health Project Floor]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

UL Certified 12V 277VAC Smart Relay for Utility MetersAuthor for correspondence