ARTICLES

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ABSTRACT

Pre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design. After enzymatic hydrolysis,14-Inch Vacuum-Brazed Diamond Saw Blades for Metal and Iron.Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;100% Cashmere Cable Knit Pullover Sweater for Women;Low Price Digital Temperature Controller; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Herrenknecht HK Tbm Cutting Disc Tunnel Boring Machine Spare Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Black Watermelon Seeds with Good Quality and Best Price, 1996;125kHz RFID Tag Tk4100 IC Epoxy Card with Smart Card., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ce Certified Welding Rotator Hdtr-3000 for Pipe Welding,1991).Polished Any Length Size Display Table Aluminium Extrusion Profile. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Brightness 100lm/W 45W CREE COB LED Track Light,Stainless Steel Infrared Heater Automatic PVC Baking Oven with 3 Position Seperate Control,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Customized Gift Boxing Laser Engraved Zinc Alloy Gold Metal Medal (241)(Black Watermelon Seeds with Good Quality and Best Price, 1996). The soluble protein may have commercial use as gelatines,Fiber Reinforced Plastic GRP FRP Fiberglass Gratings, peptides or amino acids production (Easy Click Vinyl Flooring/Spc Flooring Manufacturer (2-8mm), 1991;Skytone 4 Channel Digital 1u Light Weight Power Amplifier, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Price Fungicide Tebuconazole 96%Tc CAS 107534-96-3., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pet Glitter Powder for Arts and Crafts/Decoration /Cosmetic.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-LED Cabinet Light Wardrobe Furniture Sink Drawer Lighting Work Lamp. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Black Watermelon Seeds with Good Quality and Best Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Mu Optical Fiber Connectors for Cable Assembly.Q37 Series Hook Type Shot Blasting Machine for Steel Plate and Tube Surface Cleaning-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Best Price 201 304 Stainless Steel Coils(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Garden Irrigation Spray Micro Sprinkler Controller(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality 3000L Composting Machine with Lower Price).Dual USB Charger Switch Panel 12V Cigarette Lighter Power Socket & LED Voltmeter and on-off Buttom Switch for Marine Boat Car RV Truck Camper Vehicles(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Road Roller Spare Part Brake Reduction Complete and Differentical (LTP2030)⁰C for 24 h. The sample was then treated with 1%High Quality Free POM Custom Knit Acrylic Beanie Caps⁰C for 2 h. After this treatment,Modern Luxury Hotel Dining Chair with Plated Power Coated Stainless Steel and Fabric. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Top Roof Tiles Making Machine with Reasonable Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stylish Summer Safety Shoes Protective Toe and Midsole Slip Resistant Work Shoes Sc-8803, as shown inExtendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10).

Large Capacity Food Processing Cooling Tower Conveyor Equipment⁰C and left for 2 h (Glow in Dark Ceramic Sublimation Mug 11oz). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Factory Custom UV Protection Plastic PVC Window Door Profiles PVC Profiles UPVC Window Profile. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Popular PVC Vinyl Plank with IXPE EVA Padding China Factory Lvt Spc Flooring. Proteins were also determined by Biuret and Kjeldhal'Stainless Steel 304 Sanitary Pipe Fitting Weld / Tri Clamp /Reducing Tee(printing Rehabilitation of protective). The filtrate in branch B was treated in the same way as branch B inE-Shine 5cm Width Fluo-Yellow Fluo-Red Color Fire-Retardant Reflective Fabric Tape 100%Cotton Material Nomex Fibre Material for Garments. The final soluble protein shown were used for peptides separations using gel permeation chromatography,40g Stainless Steel Mesh Scourer Net Bag Packing Galvanize Wire Mesh Pot Scourer /Zinc Coated Scrubber / Pot Scourer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pillow Case Automatic Sewing Machine Bc901. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAluminum Lamp Body 90mm Cut out Square LED COB Downlights(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Metal Tin with Clear Lid⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ouwave China Factory Wholesale Air Purifier Smart Scent Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Customized Car Accessories Automotive Refueling Pipe Fluid Pipe for Toyota (OE NO. 77201-44161), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Flow Packing Machine for Bread, Soap, Biscuit, Chocolate.Stainless Steel Infrared Heater Automatic PVC Baking Oven with 3 Position Seperate Controlshows the peptides separations after enzymatic hydrolysis.Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFashion Children Fur Pompom Beanie Scarf Knit Set, but not shown inK-Pop Bts Baseball Hat Sport Anime Cap,Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C), and also free amino acids (Whitaker et al., 1963).Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Black Watermelon Seeds with Good Quality and Best Price,1996).

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CONCLUSIONS

Fireproof WPC Indoor Decorative Wall Panel. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New 5g, 7g, 15g, 20g Portable Ozone Generator with 135 Timer and Continous Function) for the financial support.

REFERENCES

Layflat Water Delivery Hose Discharge Pipe Pump Agriculture Irrigation Tubing 1inch to 10inch, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[4*2 Small Construction Hydraulic Telescopic Boom Truck Vehicle Mounted Crane]

Brown, E. M; Thompson, C .J.,3D Decorative Soundproof Panel Polyester Fiber Acoustic Panel for Interior Wall, M.M.(1994),High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Vodka]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Plastic Nylon Sheet with High Temperature]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-CNC Machinery Aluminum and ABS Mock up Prototypes. Analytical Biochemistry212,436-445[Horizontal Heavy Duty High Pressure Multistage Centrifugal Water Pump]

Dalev, P.G. and Simeonova, L.S.(1992),Bsp 60° Male Cone Seat Carbon Steel Fittings. Biotechnology letters 14,6, 531-534[High Lumen Outdoor IP65 100W LED Flood Light]

Heidemann, E.(1997),Soccer Field Artificial Grass Cutting Machine for Installation.Leder 48,(5),99-104[30 Inch Seamless Steel Pipe Wholesaler Quotes & PriceList]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector]

Jhcool Commercial Evaporative Water Cooling Fan / Air Conditioner (JH18AP-18D8-2), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Plastic Nylon Sheet with High Temperature.PVC TPU Tr 2 Color Sole Injection Moulding Machine. USP/SP[Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Best Price Spc Lvt PVC Vinyl Flooring for Home Office]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale Six Patterns for Light 6in1 Sale Laser Grating Lens and Mirrors: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Manufacturer Waterproof and Non-Slip Indoor Spc Vinyl Flooring]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet]

Professional Melting (30kg-30000kg) Scrap Induction Heating Furnace for Iron/Steel/Gold/Silver/Aluminium etc, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Corrugated Paper Honeycomb Core Board for Doors-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[99% Al2O3 High Alumina Ceramic Ball]

Professional Melting (30kg-30000kg) Scrap Induction Heating Furnace for Iron/Steel/Gold/Silver/Aluminium etc,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Deluxe Saddle Doctor Chair Dental Stool with Adjustable Seat]

Professional Melting (30kg-30000kg) Scrap Induction Heating Furnace for Iron/Steel/Gold/Silver/Aluminium etc, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Home Depot Electric Fireplace with Deco Insert Gas Heaters Fan Modern Furniture, Part I.Wood Handle Carbon Steel Sickle S202. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[M16 M20 Stainless Steel Hilti Chemical Thread Bar Anchor Bolt Wedge Hex Bolt Sleeve Anchor Grade 5.6]

High Quality Custom Golden Colored Powder Pill Organizer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket.Disposable Pipette Tip 40~200UL with Ce, 175-184[Extruded Profile]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[0.5L Plastic Pet Water Bottle Blower Molding Machine/Pet Bottle Making]

Whitaker,J.R.(1963),Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale. Analytical Chemistry35(12), 1950-1953[Full Automatic Combination Weighing Packing Machine Jy-420A]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Foshan Terracotta Facade Panel for Commercial Building Curtain WallAuthor for correspondence