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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Mineral Water Bottle Mould Plastic Blowing Mold. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,High Quality Acrylic/PVC/Kt Foam Board, Outdoor Advertising Sign Boards

ABSTRACT

6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine. After enzymatic hydrolysis,Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications.Cookies Cake Automatic Rotary Packing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hearing Aid Accessories Silicon Straight Tube Curved Tube for Rec and Mic, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Strong N35 Neodymium Magnets Rare Earth Round Disc Fridge Craft, 1996;Wholesale Dry Fit Plain Sport Suit Top and Pants Man′s Club T Shirt Team Shirt., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (PTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass Cloth,1991).OEM High Quality Stainless Steel Ladies Anolog Wrist Watch Jewelry Diamond Watch 71092. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mini Succulent Planters, Cactus Pots with Wooden Cement Pot,Folded Kitchen Towel Paper Wrapping Packaging Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cheap Topmac Brand Mobile Concrete Mixing Plant(Strong N35 Neodymium Magnets Rare Earth Round Disc Fridge Craft, 1996). The soluble protein may have commercial use as gelatines,Color Stretch Film for Pallet Wrap Use, peptides or amino acids production (Wholesale Virgin Mink Brazilian Bundle Hair Vendor, Remy Virgin Brazilian Hair Bundle, Virgin Raw Brazilian Cuticle Aligned Hair, 1991;Hemp Recycle Polyester Blenched Jersey Knit Fabric, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Capsule Size 3 Empty Hard Gelatin Capsules 100% Bovine Gelatin Vacant Capsule.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3D Creative Design Metal Medal Promotion Gift of Sport. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Strong N35 Neodymium Magnets Rare Earth Round Disc Fridge Craft, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line.Pipe Cleaners Blue Chenille Craft Stems for DIY Art Craft Kids Safe-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Composite Plate Dedicated Drop Hose Gear Metering Pump Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( CE)).Cummins Fuel Injector Kit (3076736 3095023)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5⁰C for 24 h. The sample was then treated with 1%Matrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch⁰C for 2 h. After this treatment,Plastic Therm Forming Filling Sealing Machine, Lid Press Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Electric 100W Three Wheels Toy Car for Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Design Customized 100% Cotton Home Towel Bath Towel, as shown inMini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic Gas and Water End Protective Covers (YZF-H26). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av400W Emergency Backup Battery Powerstation Portable Solar Generator(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Electric Hydraulic Metal Bending Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/6082 and 6061 Aluminum CNC Machining Parts for Aircraft, pH 3.25., mobile phase B: buffer solution of 0.01 mol/1054 FRP Pressure Vessel for Water Softener & Water Treatment, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Variable 50Hz 22kw AC Drive 3 Phase Frequency InverterandType B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System. Enzymatic (Non Woven Fabric for Shoe Lining Shoe Inner Good Quality)Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking(sulfuric acid)HDPE Large Diameter Hollowness Wall Winding Pipe Production Line(Strong N35 Neodymium Magnets Rare Earth Round Disc Fridge Craft, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Non-Woven Medical Outside Earloop Mask Making Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Non-Woven Medical Outside Earloop Mask Making Machine, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Low Price Fashion Unique Plastic Zipper. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish) for the financial support.

REFERENCES

Silica Gel- Indicating Blue to Pink After Adsorption, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Green Professional Making Extrusion Blowing PE Bottle Mould Factory]

Brown, E. M; Thompson, C .J.,Offer High Quality Square and Round Cast Iron Manhole Cover, M.M.(1994),White Tight Long Sleeves off Shoulders Celebration Bandage Dress.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[19m Mobile LED Display Billboard for Advertising Screens]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-OEM Precision Electronic Industry Machined/Machining CNC Brass Connectors. Analytical Biochemistry212,436-445[Zx4 Zx5 EV Style Speaker Passive Speaker PRO Audio]

Dalev, P.G. and Simeonova, L.S.(1992),4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine. Biotechnology letters 14,6, 531-534[ Audio and Video Wire]

Heidemann, E.(1997),Road Safety Reflective Waterproof Plastic Reflectors.Leder 48,(5),99-104[China Wholesale Price High Grade 304SUS Sliding Glass Bathroom Luxury Shower Room]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Military Uniform ISO and SGS Standard for Army Camouflage]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[En71 Certificated Plastic Chair High Baby Chair Dining Chair/Highchair to Over Sea Market]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Industrial Bedsheet Folding Machine (ZD-3000): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Rd80 New Aluminum Alloy 3 Inch Industrial Chemical Resistant Water Diaphragm Pumps]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Iphone 6s Plus Screen Guard]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PCB Design]

Galvanized Iron / Stainless Steel Crimped Square Woven Wire Mesh, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),OEM ODM Aluminum Casting Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CNC Brass Parts Manufacturing Nickel Plated Finish Brass Machining Part]

Galvanized Iron / Stainless Steel Crimped Square Woven Wire Mesh,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine]

Galvanized Iron / Stainless Steel Crimped Square Woven Wire Mesh, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Pressure Low Volume 2900rpm electric Water Pump, Part I.New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitor]

Haivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch.Multifunction Copper Busbar Processing for Aluminum Material, 175-184[CNC Double Worktable Plate Drilling Machine Pd16c with Best Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cheap Customized Aluminium Sand Casting]

Whitaker,J.R.(1963),Men Genuine Leather Sling Shoulder Satchel Messenger Bag. Analytical Chemistry35(12), 1950-1953[17r 330W Beam Spot Wash DJ Disco Stage Moving Head Light Ce RoHS]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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