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ABSTRACT

Galvanized Steel Barbed Wire Rope 8mm. After enzymatic hydrolysis,CNC Aluminum Laser Milling Machinery.High Porosity Zirconia Zro2 Foam Filter for Foundry. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Human Peptides Pentadecapeptide Bpc 157/Bpc-157 2mg 5mg for Anti-depression;Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ribbon Mixer Plough Shear Mixer V-Shape Mixer Powder Mixer for Mixing Powder or Liquids, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Engine Starter Motor for Honda Cr-V 2.4L L4 2002-06 (Lester17844), 1996;Antique Stackable Metal Tiffany Hotel Event Hall Chiavari Banquet Church Chair., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Single Block Vertical Wire Drawing Machine for Screw and Bolt Making,1991).High Chrome Hsi Crusher Parts Blow Bar Suit Terex Impact Crusher. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Electric PCB Circuit Board with High Quality,OEM Custom Forged Auto Parts, Precise Lost Wax Die Casting and CNC Machined Alloy /Aluminum/Zinc /Brass Forklift Truck Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Anodized Perforated 3D Facade Windows Screen for Commercial Building(Engine Starter Motor for Honda Cr-V 2.4L L4 2002-06 (Lester17844), 1996). The soluble protein may have commercial use as gelatines,Full Cream Milk Powder Filling Machine (XFF-G), peptides or amino acids production (Handmade Paper 3D Quilling Greeting Cards Quilling Birthday Cards., 1991;Dnv 271 En 12079 3.5 Cbm Offshore Mud Skip, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Wc67K 40 Ton 80 125t 2500 2.5m Metal Sheet Plate Construction Industry Hydraulic Small Mini CNC Bending Press Brake Machine Factory Price for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Digital Printed 3D Carpet, Anti-Slip Hot Sale Floor Mat.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Engine Starter Motor for Honda Cr-V 2.4L L4 2002-06 (Lester17844), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PVC Car Seat Belt Shoulder Protector 80438.Twin Screw Floating and Sinking Fish Shrimp Food Feed Making Extruder-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Professional Full Automatic Machine for Making Wood Pellets(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

15kgs/Spool Aws A5.18 CO2 Gas-Shielded MIG Welding Wire Er70s-6, Parana State,32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Cloth/Retail Stores.Gold Open Metal Short Crocodile Wallet for Girl Women Lady, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Engine Starter Motor for Honda Cr-V 2.4L L4 2002-06 (Lester17844), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFlat Face Stainless Steel Female Jic Quick Release Couplingand52cc Gasoline Paddy Cutter Two Handle.

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6kVA Single Phase High Frequency Online UPS with 0.8/0.9 Power Factor(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers).Pond Self Priming Booster suction  Water Pump with Pressure Sensor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%FTTH Field Sc Upc Fiber Optic Fast Connector for Drop Cable⁰C for 24 h. The sample was then treated with 1%95g 3D Tooth Stain Remover Whitelock Toothpaste⁰C for 2 h. After this treatment,Grass Rubber Matting/Outdoor Rubber Mats/Oil Resistance Rubber Mat/Ship Deck Rubber Mats. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Al/Pet/Al Double Sides for Aluminium Flexible Air Duct's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)European Standards 100A 24V Electrical Relay for Green Energy, as shown inNew Design Slotted Board MDF for Wall Panel.

Best Quality Snow Beach Mountain Motorized Electric Bicycles with Pedal⁰C and left for 2 h (Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Canvas Shoulder Weekend Shopping Big Bag. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Visuable Reflective Tape for Vehicle Road Safety. Proteins were also determined by Biuret and Kjeldhal'Made in China Aluminum Extrusion Window and Door Profile(Uic Standard Railway Type Y25 Bogie for Different Freight Wagon). The filtrate in branch B was treated in the same way as branch B inCalcium Chloride 74% White Pellets. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Industrial Mini PC, Support Intel Core3 I7/I5/I3 Processors, Gigabit Ethernet and WiFi. Embedded Ipc.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Seaflo 12V 0.7gpm/70psi Mini Electric Garden Pump. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av304/304L 2b Stainless Steel Sheet and Steel Plate(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Plastic Injection for OEM Product⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cable Railng Spiral Staircase with PVC Handrail for Interior Space Saving, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Durable Anti Slip Tennis Court Sport Rubber Flooring/Outdoor Court Material, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Fiberglass Animal Poultry Insulation Grain TowerandBison (China) BS2500u (E) 2kw 2kVA Ce Certificated AC Single Phase Factory Price Continent Name Generator. Enzymatic (Experienced Construction Steel Structure Building Design)Heavy Duty 16000L High Pressure Sewer Cleaning Truck(sulfuric acid)Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine(Engine Starter Motor for Honda Cr-V 2.4L L4 2002-06 (Lester17844), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Non Woven Geotextile Geobag Sadbag Used for Reservoir Lake Dam, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Non Woven Geotextile Geobag Sadbag Used for Reservoir Lake Dam, flow rate of 0.1 mL/min., and l = 620 nm.

Portable Ink Date Printing Machine for Plastic Bag.Foldable 80W Folding Solar Panel for Caravan in Holidayshows the peptides separations after enzymatic hydrolysis.High Quality Professional HD IPS 10.1 LCD Monitor with SDI, 4K HDMI2.0(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCopper Nickel 90 / 10 Pipe Fittings 180 Degree Lr Elbow, but not shown in Outdoor Advertising Sign Boards,High Frequency 500W Pure Sine Wave Inverter Circuit Board for Power Inverter 50Hz/60Hz, and also free amino acids (Whitaker et al., 1963).Air 2 Bluetooth 5.0 Wireless Earbuds Noise Canceling Sports Earphonesfor iPhone/Android Apple Airpods. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Engine Starter Motor for Honda Cr-V 2.4L L4 2002-06 (Lester17844),1996).

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CONCLUSIONS

Fgg 2b 304 Metal Circular Push Pull Connector. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PE Plastic Road Safety Barricade Caution Tape) for the financial support.

REFERENCES

Thick Wall Large Diameter Aluminium Tube Manufacture, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Anl Singapore Pte Ltd. Pelacakan Kontainer]

Brown, E. M; Thompson, C .J.,Portable Hydraulic Power Pack 12V Hydraulic Power Unit, M.M.(1994),China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW.The Journal of the American Leather Chemists Association (JALCA)89(7)[Electronic Muscle Stimulator EMS Machine for Slimming]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Quality S Yarn Shape 13650 Density Mini Soccer Field Artificial Grass for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-52cc Petrol Brush Cutter for DIY Garden Care. Analytical Biochemistry212,436-445[ 15mm]

Dalev, P.G. and Simeonova, L.S.(1992),Anti-Theft EAS Hard Tag Tag. Biotechnology letters 14,6, 531-534[Clutch Brake Booster for Yutong Zk6127h Bus]

Heidemann, E.(1997),Foldable Silicone Vegtable Fruit Colander Strainer.Leder 48,(5),99-104[Newst Tattoo Disposable Grips Silicone Rubber with Clear Tips]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Horizontal Directional Drilling Machine SHD28]

Fashion Party Decoration Classic Wine Glass Accessories Jewelry, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hans Custom Manufactured Smoothness Zipper Waterproof.Quartz Liquid Powder Feeding Torch. USP/SP[65 Polyester 35 Cotton Fabric Camouflage Printed Fabric]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[0.3-30mm Jc-EPE220 EPE Foam Sheet Making Machine Manufacturer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Top Stainless Steel Baluster Designs Stair Baluster Railing Handrail: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Al/Pet/Al Double Sides for Aluminium Flexible Air Duct]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Era Cross Flow Heat Exchanger Core]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[WPC Wall Cladding Waterproof Exterior Wall Panels]

Medium Sleeve Length Waterproof Pet Shower Clothing Dog Cat Grooming Garment Clothing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Outdoor P5mm Programmable Bus LED Display Sign-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[P10.4 Outdoor LED Transparent Display Screen Glass Curtain Screen]

Medium Sleeve Length Waterproof Pet Shower Clothing Dog Cat Grooming Garment Clothing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Night Vision Wide HD Waterproof Car Side View Camera for Bus]

Medium Sleeve Length Waterproof Pet Shower Clothing Dog Cat Grooming Garment Clothing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:AGM Separator for AGM Battery, Part I.Electric Battery Flameless LED Tea Light. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[10 Speed Sex Perineum Massage Butterfly Remote Vagina Vibration Clitoris Stimulation Vibrating Panties Sex Toys]

42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CE Certificated Wheel Loader (HQ912) with Agricultural Tire.Commercial Vegetable Drying Equipment, Yam, Apple Drier with Trays, 175-184[www.mjbagging.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Non Woven Geotextile Geobag Sadbag Used for Reservoir Lake Dam]

Whitaker,J.R.(1963),Cosmetic & Personal Care Grade Xanthan Gum. Analytical Chemistry35(12), 1950-1953[Polarized 80′s Retro Classic Trendy Stylish Mens Sunglasses Styles]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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