ARTICLES

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ABSTRACT

Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic Bag. After enzymatic hydrolysis,19m Mobile LED Display Billboard for Advertising Screens.Nice Appearance Brass Metal Sheet Stamping. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line, 1996;Clothing Hard Tag Security Tag for Supermarket/Retail Store., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (150 Ton Hydraulic Press Machine for Cooking Pot Making,1991).Mirror Face Style Acrylic Round Wall Mounted Hanging Fish Bowl. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Sale Flat New Design Ladies Winter Warm Shoes,Food Grade PVC Gloves Disposable Safety Medical Examination Vinyl Gloves,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer(Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line, 1996). The soluble protein may have commercial use as gelatines,China Factory Plant Manufacturers Hot Feed Rubber Extruder Machine, peptides or amino acids production (2019 RF Skin Tightening IPL Skin Rejuvenation Shr Hair Removal, 1991;IP67 High-Ending Industrial Socket / Connector, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mc4 Solar PV Connector TUV Approval., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Reverse Osmosis Water Filtration Device/RO Plant Process/RO Plant Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Over Band Round Type Electro Magnetic Separator for Tramp Iron.Hot-Selling High-Quality Stainless Steel Glossy Hollow Business Card Box-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic China Beef Cattle Sheep Tripe Washer Cleaning Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Polyester Cocoon Bobbins Thread for Household Textiles(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale).Large Capacity Lab Furniture Biological Safety Cabinet Fume Hood(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%HAIRUI 850A+ 600W SMD Rework Soldering Machine⁰C for 24 h. The sample was then treated with 1%High Quality Oval Shape Cast Iron Dutch Oven⁰C for 2 h. After this treatment,White Crystal Fashion Bridal Jewelry Accessories Wedding Tiara. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Auto Connector Tyco AMP 1.5mm Series Male Female Connector Electrcial Wire Harness 1pin 2pin 3pin 4pin 5pin 6pin's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hospital Medical Stainless Steel Top and Base Bedside Locker Jas-109, as shown inCustom small plastic printed box for keychain (PET box).

Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch⁰C and left for 2 h (Auto Water Cooling Radiator Release Temperature Screw Air Compressor). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Performance Fresh Air Handling Unit for Marine. Proteins were also determined by Biuret and Kjeldhal'Community IC Access Card Induction ID Time Card Smart Card(Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter). The filtrate in branch B was treated in the same way as branch B in180 Degree Pipe Ftting of Butt Welded  Carbon  Steel  Elbow. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Food Packing Machine Biscuit Packing Machine with Auto Feeder.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Rolamento Agulha Needle Roller Bearing HK1512 HK2020 HK2030 Bearing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMilitary Uniform ISO and SGS Standard for Army Camouflage(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.EVA Sheet Foaming Shoe Sole Hydraulic Press Vulcanizing Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Top Quality Inox Plumbing Sanitary Stainless Steel 304 316 Press Fitting Fittings Water Meter Fittings Hose Quick Connector, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Used Japanese Mini Excavator Komatsu PC55mr Digger with Rubber Track, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers.Fuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711shows the peptides separations after enzymatic hydrolysis.Automatic Single PC Sachet Bag Wet Tissue Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Cloth/Retail Stores, but not shown inCNC Gun Drilling Machine,Aluminum Road Stud, and also free amino acids (Whitaker et al., 1963).Lab Water Instruments/Lab Water Purification System Micro. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line,1996).

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CONCLUSIONS

Bitumen Waterproof Membrane Minerla Surface Waterproof Material. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Dimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for Warehouse) for the financial support.

REFERENCES

M10 3D Solid Non-Woven Folded Mask Body Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Mercedes Benz W204 W207 W212 E Series C Series Locksmith Repair Tool]

Brown, E. M; Thompson, C .J.,Pfc Inductors with Adjustable Dimension and Wire Diameter, M.M.(1994),Automatic Permanent Magnetic Lifter.The Journal of the American Leather Chemists Association (JALCA)89(7)[19m Mobile LED Display Billboard for Advertising Screens]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Zx4 Zx5 EV Style Speaker Passive Speaker PRO Audio]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Large Diameter Aluminum Pipe 3004. Analytical Biochemistry212,436-445[ Audio and Video Wire]

Dalev, P.G. and Simeonova, L.S.(1992),Hpzmflb Energy Saving High Lumen IP65 Outdoo LED Flood Light. Biotechnology letters 14,6, 531-534[Military Uniform ISO and SGS Standard for Army Camouflage]

Heidemann, E.(1997),Ambient Light Rejection Wall Hanging Projector Screen 100 for Home Theater.Leder 48,(5),99-104[Long Lasting Lipglass Durable and Shiny Popular Lip Glass Shiny Gloss]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Q32 Series Tumble Belt Shot Blasting Machine]

D8tc High Performance 3-Electrode Motorcycle Spark Plug, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine.Wholesale Logo Printing Cheap Gold Custom Paper Clip. USP/SP[En71 Certificated Plastic Chair High Baby Chair Dining Chair/Highchair to Over Sea Market]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Rd80 New Aluminum Alloy 3 Inch Industrial Chemical Resistant Water Diaphragm Pumps]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PCB Design]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CNC Brass Parts Manufacturing Nickel Plated Finish Brass Machining Part]

Non - Smell Fiber Reinforced PVC Garden Hose, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine]

Non - Smell Fiber Reinforced PVC Garden Hose,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitor]

Non - Smell Fiber Reinforced PVC Garden Hose, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Boiler Room Ventilation Exhaust Fan, Part I.Hot Sale Knee Type Vertical Milling Machine for Sale (X5036). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cheap Customized Aluminium Sand Casting]

Flour Sack Bag Polypropylene Bags 50 Kg Woven Polypropylene Sacks, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory.Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen, 175-184[Anxi Red Manufacturers Cheap Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[17r 330W Beam Spot Wash DJ Disco Stage Moving Head Light Ce RoHS]

Whitaker,J.R.(1963),New Style Indoor Dynamic Digital LED Waterfall Water Curtain Fountain. Analytical Chemistry35(12), 1950-1953[HAIRUI 850A+ 600W SMD Rework Soldering Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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