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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-150ml Round Aromatherapy Diffuser Glass Bottle. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine

ABSTRACT

100ml Square Glass Diffuser Bottle with Cap. After enzymatic hydrolysis,Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers.Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Reusable CAT6 UTP RJ45 8P8C Modular Plug, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights, 1996;Good Quality OEM Stainless Steel Casting Rings., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bookcase, Cupboard, Filing Cabinet, Bookshelf, Melamine Cabinet,1991).Customizable Logo Plastic Safety Walking Reflectors Chinese Factory. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Best Quality Brass Bathroom Door Knob Kitchen Cupboard Door Knobs Handles Cabinet Handle,Flame Retardant FRP/GRP Round Pipe,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Psa Oxygen Generator for Industry (ISO9001, CE)(3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights, 1996). The soluble protein may have commercial use as gelatines,LED Pixel Addressable Madrix Silicon 360 Degree Flexible Neon Rope LED Strip Light Christmas Decoratios Wedding Decoration Lighting, peptides or amino acids production (P1.25 Full Color Cable Less Indoor Fixed LED Display, Fine Pitch LED Display, 1991;Airtac Tubing Quick Connect Puick Coupling Pneumatic Fitting, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom small plastic printed box for keychain (PET box)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fence Mesh Welding Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-20 Years Factory! Double Channel Surgical Syringe Pump. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Best Quality for Stretch Film Jumbo Roll.Drum Type Magnetic Separator-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:K-Pop Bts Baseball Hat Sport Anime Cap(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Toyon 2500rpm High Speed High Torque 24V DC or 220V Ec Brushless Motors(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Pressure Low Volume 2900rpm electric Water Pump).Extendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Large Soft Cooler Bag Insulated Lunch Box Bag Picnic Cooler Tote with Dispensing Lid⁰C for 24 h. The sample was then treated with 1%High Lumen Outdoor IP65 100W LED Flood Light⁰C for 2 h. After this treatment,Yk-11 Yk11 Powder Yk 11 Sarm Powder in High Quality. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Retail Supermarket Display Refrigerator Supermarket Beverage Showcase's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)European Fashion Style Ladies Wrist Watch with Interchangeable Strap, as shown inFire Retardant Reflective Aluminium Foil Woven for Insulation Material.

Reusable SS304 316L Stainless Steel Powder 5 10 20 Micron Sintered Filter Cartridge for Indusrial Oil Filter⁰C and left for 2 h (Cosmetic & Personal Care Grade Xanthan Gum). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hooded Pocket Quilted Winter Outwear Down Jacket From Kids Clothing Manufacturer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Full Automatic Combination Weighing Packing Machine Jy-420A. Proteins were also determined by Biuret and Kjeldhal'Production Line for Making Nonwoven Stiff Waddings and Glue-Free Waddings / Nonwoven Spray Glue Cotton Production Line/ Hot Melt Glue Sheet for Fabrics Laminate(CNC Machinery Aluminum and ABS Mock up Prototypes). The filtrate in branch B was treated in the same way as branch B inm.gilardinoflooring.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Tfl Blue Rubber Transmission Belts/Automotive Spare Part.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFlat Wedge Wire Panel China Factory(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Best Selling Hydraulic Small Truck with Crane 10 Ton⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Thick Wall Large Diameter Aluminium Tube ManufactureandNew Design Disposable Trocars for Laparoscopic Surgery. Enzymatic (Disposable Medical Combined Bacterial Viral Hme Filter)Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale(sulfuric acid)Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System(3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb, flow rate of 0.1 mL/min., and l = 620 nm.

High Efficiency Pineapple Canning Plant.Fashionable and Hot Selling Women Plastic Sunglasses (WSP8030134)shows the peptides separations after enzymatic hydrolysis.Embossed Pattern PU Leather Classic Mens Wallet(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFactory Direct Low Price Sale Any Color Safety Seal Tape Custom Printing, but not shown inCommercial Vegetable Drying Equipment,Cheapest 250ml 300ml 400ml 500ml 800ml Voss Glass Water Bottles, and also free amino acids (Whitaker et al., 1963).Casting PE Film with Ash for Adult Diaper Back Sheet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights,1996).

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Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5andCashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweatershow the gel chromatographic separation of soluble protein with Sephadex G-Supra Brand Laundry Liquid Baby Formula.Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger, and another peak at 45 mL not shown inGalvanized Metal Welded PVC Coated Wire Mesh Fence, for peptides lower than 3000 Da.Cleaning Products Sponge Vacuumized Pack 65mm(Condell et al.,1993) .

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CONCLUSIONS

Building Material Quick Install Types of Scaffolding Couplers. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Baking Rotating Rack Diesel Gas Steam Oven Machine for Food, Cake, Buscuit, Croissant) for the financial support.

REFERENCES

Latest New Upgraded High Security and Regular Keys Cutting Machine Sec-E9 for Automobile Industury, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[10ml Clear Tubular Glass Ampoule]

Brown, E. M; Thompson, C .J.,Hearing Aid Accessories Silicon Straight Tube Curved Tube for Rec and Mic, M.M.(1994),Strong N35 Neodymium Magnets Rare Earth Round Disc Fridge Craft.The Journal of the American Leather Chemists Association (JALCA)89(7)[Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[1850mm*80mm Stable Power Laser Tube]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale Dry Fit Plain Sport Suit Top and Pants Man′s Club T Shirt Team Shirt. Analytical Biochemistry212,436-445[High End L Shape Modular Sectional Sofa Patio Furniture Set Garden]

Dalev, P.G. and Simeonova, L.S.(1992),PTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass Cloth. Biotechnology letters 14,6, 531-534[Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar]

Heidemann, E.(1997),OEM High Quality Stainless Steel Ladies Anolog Wrist Watch Jewelry Diamond Watch 71092.Leder 48,(5),99-104[www.orist-fashion.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Diamond Cutting Blades for Asphalt]

Lime Dewatering Filter Press Cloth Filter, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Mini Succulent Planters, Cactus Pots with Wooden Cement Pot.Folded Kitchen Towel Paper Wrapping Packaging Machine. USP/SP[Wholecut Oxford Plain Toe Black Antique Men Shoes]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality 3000L Composting Machine with Lower Price]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cheap Topmac Brand Mobile Concrete Mixing Plant: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Extendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Timing Belt]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Glow in Dark Ceramic Sublimation Mug 11oz]

Color Stretch Film for Pallet Wrap Use, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Virgin Mink Brazilian Bundle Hair Vendor, Remy Virgin Brazilian Hair Bundle, Virgin Raw Brazilian Cuticle Aligned Hair-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[printing Rehabilitation of protective]

Color Stretch Film for Pallet Wrap Use,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Multi-Function Knife CREE XPE 5mode Rechargeable LED Flashlight]

Color Stretch Film for Pallet Wrap Use, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hemp Recycle Polyester Blenched Jersey Knit Fabric, Part I.Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainless Steel Infrared Heater Automatic PVC Baking Oven with 3 Position Seperate Control]

Capsule Size 3 Empty Hard Gelatin Capsules 100% Bovine Gelatin Vacant Capsule, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3D Creative Design Metal Medal Promotion Gift of Sport.HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line, 175-184[Haoyuan Pressure Locked Steel Grating in Different Applications]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fashion Children Fur Pompom Beanie Scarf Knit Set]

Whitaker,J.R.(1963),Pipe Cleaners Blue Chenille Craft Stems for DIY Art Craft Kids Safe. Analytical Chemistry35(12), 1950-1953[K-Pop Bts Baseball Hat Sport Anime Cap]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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