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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Mini Outdoor Hydraulic Man Aluminum Lift. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Wholesale Fashion Women Clothes Fashion Yaga Pants

ABSTRACT

High Efficient Disinfection with Low Price N-Alkyl Dimethyl Benzyl Ammonium Chloride. After enzymatic hydrolysis,Economic Full Automatic LED Pick and Place Machine for LED Strip Making.1/4 25FT Twin Line Welding Hose for Oxy-Acetylene. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;PETG Acrylic Sheet for Window;High Frequency Online UPS W/3W-20K; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output, 1996;14 Head Multihead Weigher for Pork with Screw Feeding., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Sanitary SS304/SS316 2 Way Tri Clamp Ball Valve,1991).PSF 1000 Flat Plate Upper Discharge separator. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Aromatherapy Essential Oil Ultrasonic Diffuser with APP Remote Control Smart,Powerful Neodymium Pot Magnet Rare Earth Pot Magnet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CNC Gun Drilling Machine(Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output, 1996). The soluble protein may have commercial use as gelatines,Dog Puppy Squeaker Plush Toy Training Chew Simulation Pet Products, peptides or amino acids production (High Whiteness & Excellent Dispersity Anatase Type Titanium Dioxide, 1991;Large Jewelry Display Box for Ring Necklace Earring Storage Case, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Indoor Tub Customized Shower/Free Standing Shower Enclosure., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Glow in Dark Ceramic Sublimation Mug 11oz.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Double Poles Number Bimetal Strip 1AMP Mini Circuit Breaker. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel 201/304/316 CF8/CF8m 1000wog Threaded Ball Valve.22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Supermarket Combined Island Chest Freezer Commercial Refrigerator(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Concrete Road Crack-Pouring Machine for Construction, Parana State,Wholesale Fashion Women Clothes Fashion Yaga Pants.Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the 750mm Cuttable Capacitive Diesel Fuel Level SensorandWholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar.

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China OEM Alloy Steel Precision Casting(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Different Wood Options Rigid Spc Core Vinyl Flooring).Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Best Quality Hose Crimping Machine⁰C for 24 h. The sample was then treated with 1%1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitor⁰C for 2 h. After this treatment,Galvanized Metal Welded PVC Coated Wire Mesh Fence. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Butyl Tube 9.5r20, 12.4r24, 23.1r26, 16.9r38 Dong Ah Brand's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)720p WiFi Wireless Smart Home Security Two Way Audio HD Night Vision Baby Monitor Support TF Card Onvif IP Camera, Video Camera, as shown inGood Quality OEM Stainless Steel Casting Rings.

Luzhong-700 70HP 2WD Two Wheel Drive Farm Tractor⁰C and left for 2 h (Indoor Tub Customized Shower/Free Standing Shower Enclosure). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Original SKF Deep Groove Ball Bearings 6208 6208-2z SKF 6208-2z. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Top Quality Sanitary Tube Shell Tube Heat Exchanger. Proteins were also determined by Biuret and Kjeldhal'Different Thickness Mirror Color Coated Almn1cu Almg2.5 Aluminium Coil with PVC(Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer). The filtrate in branch B was treated in the same way as branch B inCar Inspection Gauge of Interior Parts. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Xinan Delphi 9308-621c Diesel Injection Valve 9308-621c Fuel Injector Control Valve 28239294 Common Rail Valve 9308z621c.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Precision iPhone Cases Fiber Laser Marking Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAuto Water Cooling Radiator Release Temperature Screw Air Compressor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PCBA SMT Equipment PCB Depaneling Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/HDPE Pipe for Water Supply (OD: 16mm-800mm), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Nylon Hammer Fixings Drive Anchor, V_e, and K_avcalculated as discussed previously.OEM Electrolyte Effervescent Tablet,Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (European Fashion Style Ladies Wrist Watch with Interchangeable Strap) (Reis et al.,1989;High Whiteness & Excellent Dispersity Anatase Type Titanium Dioxide.,1991;14 Head Multihead Weigher for Pork with Screw Feeding.,1991).6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter(1992), were lower, except for cystine and tyrosine,Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet.

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CONCLUSIONS

20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Clip on Wire Shelving Data Strip) for the financial support.

REFERENCES

304 321 310 310S Welded Decorative Stainless Steel Pipe, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Flange Adapter Sc/APC Om4 Duplex with Metal Dust Cap Fiber Optic Coupler Laser]

Brown, E. M; Thompson, C .J.,Hot Sale! ! ! Bearing 608 Ceramic Deep Groove Ball Bearing (608ZZ/RS), M.M.(1994),Manual Electric Winch Small Winch 220V Winch.The Journal of the American Leather Chemists Association (JALCA)89(7)[Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ce Approved Cheap Medical Disposable Electrical Infusion Pump]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price. Analytical Biochemistry212,436-445[Used Japanese Mini Excavator Komatsu PC55mr Digger with Rubber Track]

Dalev, P.G. and Simeonova, L.S.(1992),Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar. Biotechnology letters 14,6, 531-534[Stainless Steel Manual 3-Way Plug Valve]

Heidemann, E.(1997),Plastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A.Leder 48,(5),99-104[Metal Laser Cutting Machine Factory & Manufacturer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Durable 25kg 50kg Red PP Woven Bag Sack]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Car Seat Pockets PU Leather Car Console Side Organizer-Orange]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Aluminum Plate Fin Heat Exchanger/Oil Cooler Core: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Tamper Evident Branded Printed Packing Pet Adhesive Security Tape]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[RFID CARD]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High quality Brass Forged Gate Valve with Casting Iron Handle]

3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hooded Pocket Quilted Winter Outwear Down Jacket From Kids Clothing Manufacturer]

3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[1.5tons Mini Site Dumper SD15]

3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:5-Axis CNC Machining Billet Aluminum Engine Rotary Housing, Part I.EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Tongkai Classic Business Hardcover Leather Notebook]

Whitaker,J.R.(1963),Kids Folding Play Mat Kids Play Room Floor Mat. Analytical Chemistry35(12), 1950-1953[Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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