ARTICLES

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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Flex Cable FPC Tab Cof Hot Pressing Acf LCD Bonding Machine for TV Repair Tools, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ce TUV SGS BV Certificate CNC Router with Rotary Fixture China, 1996;Baby Early Learning Cards Custom Printed Happy Birthday Greeting Card and Memory Card Game with Custom Logo Package Box., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Optical Fiber Connector Cleaning Cassette Reel Cleaner, Non-Alcoholic Specialty Fibers 500+ Cleans,1991).Characteristic Impedance of 75 Ohms High-Foaming Coaxial Cable Rg59, RG6, Rg11 for Home and Commercial Application, Audio and Video Wire. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Modern Container House / Modular Residential / Prefabricated Mobile Home,100% Polyester Luxury Bedding Comforter Fleece Blanket Throw,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluminum Window System Thermal Break Strip (HK Stype35.3mm)(Ce TUV SGS BV Certificate CNC Router with Rotary Fixture China, 1996). The soluble protein may have commercial use as gelatines,Multi-Function Knife CREE XPE 5mode Rechargeable LED Flashlight, peptides or amino acids production (Jungle Camo Army Uniform Hunting Softshell Waterproof Military Jacket, 1991;Full Automatic Milk/ Chemistry/Shampoo Plastic Bottle Blowing Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Speed Mixer for Plastic Extrusion Line., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Trailer Wire Harness with 7 Pole Trailer Plug & Fuse Holder for Trailer Wiring Harness Assembly for Challonger.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-10mm Gold Plated Stainless Steel Balls G100 Polish Ball. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ce TUV SGS BV Certificate CNC Router with Rotary Fixture China, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine.Bag Accessories 10mm Metal Dog Swivel Snap Hook, Wholesale Antique Brass Trigger Snap Hook for Bags-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:304 Seamless Stainless Steel Tube(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Theme Jungle Plastic Stainless Steel Slide Outdoor Playground(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover).Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Updated Stylish Peristaltic Biogas Pump Gas Booster Pump⁰C for 24 h. The sample was then treated with 1%110V-240V LED Filament Bulb Globe Cafe Patio String Light⁰C for 2 h. After this treatment,Professional Sound System Vt215 Line Array Speaker. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Factory Price Cellulose Ether HPMC, HEC, Hpc, CMC's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)LED Alloy Aluminum Extrusion Profiles Advertising Accessories for Lighting, as shown inHydrafacial Water Cleaning Skin Peel Facial Beauty Machine.

Duct Evaporative Air Cooler for Warehouse Factory⁰C and left for 2 h (Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Micro Sprinkler for Water Saving Irrigation. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Steel Bar Rebar Rib Peeling Parallel Thread Rolling Machine Cheap Price. Proteins were also determined by Biuret and Kjeldhal'High End L Shape Modular Sectional Sofa Patio Furniture Set Garden(Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C)). The filtrate in branch B was treated in the same way as branch B inPortable Standing Desk Foldable. The final soluble protein shown were used for peptides separations using gel permeation chromatography,ABS Shell Plastic Injection Molding.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av450mm High Quality Circular Diamond Cutting Saw Blade for Marble Stone(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aluminium Profile Heavy Duty Bending Machine Aluminum Window Door Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Towed Bitumen Mixer Slj5, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Adsorption Capacity and Porosity Chloride Adsorbent Activated Alumina Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truckand3D Inkjet Printing Waterproof Interior Kitchen Wall Tile. Enzymatic (IMTEK High Carbon Chrome-Plated Gcr15 TBR20 Linear Shaft for CNC)T5577 Thick Card T5577 Security Smart Chip RFID ABS Plastic Blank Thick Card(sulfuric acid)Super Absorbent Lint Free Clean PCB Double Foam Tip Swab Stick(Ce TUV SGS BV Certificate CNC Router with Rotary Fixture China, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Viscose Sodium Sulphate Anhydrous by Sateri, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Viscose Sodium Sulphate Anhydrous by Sateri, flow rate of 0.1 mL/min., and l = 620 nm.

Orange Silica Gel 2-4 mm with High Quality. Kitchen Floor Matshows the peptides separations after enzymatic hydrolysis.2019 Top Quality USB-C Docking Station with SD Card Reader Dp VAG HDMI(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in5.5 to 7.5kw AC Frequency Inverter for Motor AC Drive, but not shown inHigh Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing,aluminium discs for fry pan alloy 1060 1100 1050 3003, and also free amino acids (Whitaker et al., 1963).High Quality Insulated Double Wall Bamboo Glass Water Bottle. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Ce TUV SGS BV Certificate CNC Router with Rotary Fixture China,1996).

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CONCLUSIONS

Eco-Friendly Round Head Screw Stud Flange Hexagon Bolt for Building. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck) for the financial support.

REFERENCES

Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Good Quality Smart Electronic Key Card Hotel Door Lock]

Brown, E. M; Thompson, C .J.,2-25mm Co-Extruded Highlight PVC Foam Board, M.M.(1994),CNC Engraving Machine Laser Marking for Assembly Line.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Customized High Quality Stainless Steel Precision Motorcycle Parts /Accessory Sample]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Embossed Lattice PU Synthetic Leather for Bags/Handbags (HS703)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PP/PE Plastic Lawn Turf Gravel Grass Grid for Driveway Paver (PGG5050A-1). Analytical Biochemistry212,436-445[Fashionable Clothing Garment Paper Swing Tags (ST-01)]

Dalev, P.G. and Simeonova, L.S.(1992),Personalized Transparent Waterproof Plastic Bag Wet Underwear Clear PVC/EVA Ziplock Packing Bag with Gusset for Swimwear. Biotechnology letters 14,6, 531-534[Telescopic Slideway Steel Protection Accordion Cover]

Heidemann, E.(1997),High Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing.Leder 48,(5),99-104[Angel Wings Shaped Mirror Antique Royal Style Home Decorative Mirror]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Galvanized Steel Barbed Wire Rope 8mm]

Fiberglass Covered Insulated Flat/Square Aluminum Magnet Wire, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),12V/24V, Ts16949, Air Conditioner Fan for Difference Refrigeration Truck.nellcor non-oximax 7pin infant disposable sensor. USP/SP[Double Wine Carrier Bag Gift Box Tote]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Plug-in Assembly Type Straight Through Joint and Insulation Joint]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Power Solar Inverter with Full Power 3000W Converter: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Featured]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[304 Seamless Stainless Steel Tube]

Custom Made EPDM FKM Rubber Mechanical Seal Diaphragm for Valve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory]

Custom Made EPDM FKM Rubber Mechanical Seal Diaphragm for Valve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Canvas Casual Men Shoes]

Custom Made EPDM FKM Rubber Mechanical Seal Diaphragm for Valve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Zoo-Tunes Portable Wireless Mini Character Speakers, Part I.CF8m Air Actuated Valve Dn15. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[One Step HBV 5 in 1 Rapid Test]

Rectangular Design Ceramic Small Glass Basin Sink for Bathroom, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Premium Baby Toddler Food Feeding Silicone Bowl with Lid.Hardcover Bear′s Story Children Book Custom Printing, 175-184[Lamella Tube Settle Clarifier for Waste Water Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Kraft Paper Laminated Aluminum Foil]

Whitaker,J.R.(1963),Deluxe Saddle Doctor Chair Dental Stool with Adjustable Seat. Analytical Chemistry35(12), 1950-1953[Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal PartsAuthor for correspondence