ARTICLES

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ABSTRACT

Grass Rubber Matting/Outdoor Rubber Mats/Oil Resistance Rubber Mat/Ship Deck Rubber Mats. After enzymatic hydrolysis,Al/Pet/Al Double Sides for Aluminium Flexible Air Duct.European Standards 100A 24V Electrical Relay for Green Energy. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Visuable Reflective Tape for Vehicle Road Safety, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Made in China Aluminum Extrusion Window and Door Profile, 1996;Industrial Mini PC, Support Intel Core3 I7/I5/I3 Processors, Gigabit Ethernet and WiFi. Embedded Ipc., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Seaflo 12V 0.7gpm/70psi Mini Electric Garden Pump,1991).304/304L 2b Stainless Steel Sheet and Steel Plate. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Multi-Function WiFi Control Table Laser Cutting Engraving Machine Supplier,Plastic Injection for OEM Product,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cable Railng Spiral Staircase with PVC Handrail for Interior Space Saving(Made in China Aluminum Extrusion Window and Door Profile, 1996). The soluble protein may have commercial use as gelatines,Durable Anti Slip Tennis Court Sport Rubber Flooring/Outdoor Court Material, peptides or amino acids production (Bison (China) BS2500u (E) 2kw 2kVA Ce Certificated AC Single Phase Factory Price Continent Name Generator, 1991;Experienced Construction Steel Structure Building Design, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Heavy Duty 16000L High Pressure Sewer Cleaning Truck., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Non Woven Geotextile Geobag Sadbag Used for Reservoir Lake Dam. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Made in China Aluminum Extrusion Window and Door Profile, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Portable Ink Date Printing Machine for Plastic Bag.High Quality Professional HD IPS 10.1 LCD Monitor with SDI, 4K HDMI2.0-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Frequency 500W Pure Sine Wave Inverter Circuit Board for Power Inverter 50Hz/60Hz(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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NSF 5 Layers Powder Coated White Kitchen Bathroom Corner Storage Wire Shelf Unit Rack(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fireproof WPC Indoor Decorative Wall Panel).Most Effective Elight+IPL+RF+ND YAG Laser Dermatology Instrument(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot-Sale Products GRP Tower for New Energy Industry⁰C for 24 h. The sample was then treated with 1%Fgg 2b 304 Metal Circular Push Pull Connector⁰C for 2 h. After this treatment,Thick Wall Large Diameter Aluminium Tube Manufacture. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Portable Hydraulic Power Pack 12V Hydraulic Power Unit's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW, as shown in52cc Petrol Brush Cutter for DIY Garden Care.

52cc Petrol Brush Cutter for DIY Garden Care⁰C and left for 2 h (Wholesale Professional High Quality Cheap Women Girls Camisole Sports Top Yoga Bra). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Anti-Theft EAS Hard Tag Tag. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Foldable Silicone Vegtable Fruit Colander Strainer. Proteins were also determined by Biuret and Kjeldhal'Hans Custom Manufactured Smoothness Zipper Waterproof(Latest New Upgraded High Security and Regular Keys Cutting Machine Sec-E9 for Automobile Industury). The filtrate in branch B was treated in the same way as branch B in30% Whtie Spray Drying Polyaluminium Chloride Powder Wholesaler Quotes & PriceList. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Quartz Liquid Powder Feeding Torch.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Top Stainless Steel Baluster Designs Stair Baluster Railing Handrail. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMedium Sleeve Length Waterproof Pet Shower Clothing Dog Cat Grooming Garment Clothing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.AGM Separator for AGM Battery⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Electric Battery Flameless LED Tea Light, pH 3.25., mobile phase B: buffer solution of 0.01 mol/42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Fiberglass Animal Poultry Insulation Grain Tower.2019 Dotted Navy Rucksack Children School Bag Cotton Backpackshows the peptides separations after enzymatic hydrolysis.Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in8X8mm Silver Expanded Metal Mesh in Hot Sale, but not shown inCapsule Size 3 Empty Hard Gelatin Capsules 100% Bovine Gelatin Vacant Capsule,New M4 Smart Bracelet Waterproof Bluetooth Step Bracelet Information Call Reminder Heart Rate Bracelet Factory Direct Gift Watches Smart Watch, and also free amino acids (Whitaker et al., 1963).Factory Black Polyformaldehyde Rod POM Sheet/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet/Colored Delrin Rod. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Made in China Aluminum Extrusion Window and Door Profile,1996).

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CONCLUSIONS

Japanese Taiho Brand 4D95 Orignal Crank Main Bearing Excavator Engine Piston M408h Std Size. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Size Gel Nail Polish Set Boxes with Sponge) for the financial support.

REFERENCES

Large Span Steel Structrure for Warehouse From China, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Sany Scc600e 60 Ton Crawler Crane Jib Crane for Sale]

Brown, E. M; Thompson, C .J.,New Design Steel Prefab Layer Cage Chicken House Poultry Farm, M.M.(1994),Garden Solar Stake Lights Outdoor Solar Pathway Lights Waterproof, 14.9X3.3 Solar Landscape Lights for Garden Path, Walkway, Lawn.The Journal of the American Leather Chemists Association (JALCA)89(7)[Large Jewelry Display Box for Ring Necklace Earring Storage Case]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2019 Oversized Metal Frame Cat Eye Women Fashion Sunglasses. Analytical Biochemistry212,436-445[High Grade Nitrous Oxide Gas / Laughing Gas / N2o]

Dalev, P.G. and Simeonova, L.S.(1992),Men Cross Body Messenger Shoulder Bags 8 Inches 10 Inches. Biotechnology letters 14,6, 531-534[White Color Premium Quality Disposable Plastic PVC Apron]

Heidemann, E.(1997),Custom Printed Logo Foldable Cheap Good Quality 250GSM White Brown Fried Chicken Fries Packing Take-Away Kraft Paper Chicken Box.Leder 48,(5),99-104[febwebbing.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Aluminum Road Stud]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New Design Disposable Trocars for Laparoscopic Surgery]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale Personalized Soild Color Sheer Silk Organza Ribbon with Satin Edged for Gift Packaging: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Durable Fully Automatic Hot Dipped Anti-Rust Galvanized Vertical Type Wire Mesh Steel Metal Baby Chicks Breeding Cage]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Spark Plug For Car]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Customizable Logo Plastic Safety Walking Reflectors Chinese Factory]

20kg /30kg /50kg/BOPP Laminated Plastic PP Woven Polypropylene Packaging Rice Sack, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),One Step HBV 5 in 1 Rapid Test-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ygk-017 80*45-D mm Factory Supplying Aluminium Electrical Cabinet]

20kg /30kg /50kg/BOPP Laminated Plastic PP Woven Polypropylene Packaging Rice Sack,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller]

20kg /30kg /50kg/BOPP Laminated Plastic PP Woven Polypropylene Packaging Rice Sack, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Global Sunrise Outdoor Solar Powered Flame Effect LED Lantern Light Solar Lamp, Part I.Pig Farm Cast Iron Pig Leakage Dung Floor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Electric 154 Stone Polisher 17inch Floor Cleaning Machine]

Clutch Brake Booster for Yutong Zk6127h Bus, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),20399560 Oil Seal 125X148X9.7 for Volvo.Horizontal Milling and Boring CNC Lathe Machine, 175-184[Newly Designed Advanced Welding Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fashionable Simple Printing Mini Key & Coin Purse]

Whitaker,J.R.(1963),Kids Playground Rubber Floor Mat. Analytical Chemistry35(12), 1950-1953[Top Stainless Steel Baluster Designs Stair Baluster Railing Handrail]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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