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ABSTRACT

6kVA Single Phase High Frequency Online UPS with 0.8/0.9 Power Factor. After enzymatic hydrolysis,Pond Self Priming Booster suction  Water Pump with Pressure Sensor.FTTH Field Sc Upc Fiber Optic Fast Connector for Drop Cable. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Al/Pet/Al Double Sides for Aluminium Flexible Air Duct, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (European Standards 100A 24V Electrical Relay for Green Energy, 1996;Best Quality Snow Beach Mountain Motorized Electric Bicycles with Pedal., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Canvas Shoulder Weekend Shopping Big Bag,1991).High Visuable Reflective Tape for Vehicle Road Safety. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Made in China Aluminum Extrusion Window and Door Profile,Industrial Mini PC, Support Intel Core3 I7/I5/I3 Processors, Gigabit Ethernet and WiFi. Embedded Ipc,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Seaflo 12V 0.7gpm/70psi Mini Electric Garden Pump(European Standards 100A 24V Electrical Relay for Green Energy, 1996). The soluble protein may have commercial use as gelatines,304/304L 2b Stainless Steel Sheet and Steel Plate, peptides or amino acids production (Multi-Function WiFi Control Table Laser Cutting Engraving Machine Supplier, 1991;Plastic Injection for OEM Product, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cable Railng Spiral Staircase with PVC Handrail for Interior Space Saving., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Durable Anti Slip Tennis Court Sport Rubber Flooring/Outdoor Court Material.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Bison (China) BS2500u (E) 2kw 2kVA Ce Certificated AC Single Phase Factory Price Continent Name Generator. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (European Standards 100A 24V Electrical Relay for Green Energy, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Experienced Construction Steel Structure Building Design.Heavy Duty 16000L High Pressure Sewer Cleaning Truck-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Custom Size Gel Nail Polish Set Boxes with Sponge(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Horizontal Milling and Boring CNC Lathe Machine).High Precision Ce Radial Drill Machine Z3040(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Carbon Fiber Soft TPU Shockproof Back Cover Case for Huawei Honor V10 /Honor V9⁰C for 24 h. The sample was then treated with 1%Dual Frequency RFID Key Cards for Both Hotel Elevator & Room⁰C for 2 h. After this treatment,NSF 5 Layers Powder Coated White Kitchen Bathroom Corner Storage Wire Shelf Unit Rack. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Most Effective Elight+IPL+RF+ND YAG Laser Dermatology Instrument's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot-Sale Products GRP Tower for New Energy Industry, as shown inCog Mono Round Display LCD Module.

Fgg 2b 304 Metal Circular Push Pull Connector⁰C and left for 2 h (Wholesale Waterproof Outdoor Army Military Camouflage Printing Ripstop Polyester Fabric). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Thick Wall Large Diameter Aluminium Tube Manufacture. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Portable Hydraulic Power Pack 12V Hydraulic Power Unit. Proteins were also determined by Biuret and Kjeldhal'China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW(Tfl Blue Rubber Transmission Belts/Automotive Spare Part). The filtrate in branch B was treated in the same way as branch B inButterfly Valve Control Wholesaler Quotes & PriceList. The final soluble protein shown were used for peptides separations using gel permeation chromatography,52cc Petrol Brush Cutter for DIY Garden Care.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Anti-Theft EAS Hard Tag Tag. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFoldable Silicone Vegtable Fruit Colander Strainer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Quartz Liquid Powder Feeding Torch⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Top Stainless Steel Baluster Designs Stair Baluster Railing Handrail, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Medium Sleeve Length Waterproof Pet Shower Clothing Dog Cat Grooming Garment Clothing, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

150 micron steel mesh filter magnetic hydraulic filter cartridgeandOutdoor P5mm Programmable Bus LED Display Sign. Enzymatic (AGM Separator for AGM Battery)Electric Battery Flameless LED Tea Light(sulfuric acid)42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor(European Standards 100A 24V Electrical Relay for Green Energy, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%CE Certificated Wheel Loader (HQ912) with Agricultural Tire, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%CE Certificated Wheel Loader (HQ912) with Agricultural Tire, flow rate of 0.1 mL/min., and l = 620 nm.

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Silica Gel- Indicating Blue to Pink After AdsorptionandElectric 100W Three Wheels Toy Car for Saleshow the gel chromatographic separation of soluble protein with Sephadex G-Fiberglass Animal Poultry Insulation Grain Tower.Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts, and another peak at 45 mL not shown inAuto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV, for peptides lower than 3000 Da.New M4 Smart Bracelet Waterproof Bluetooth Step Bracelet Information Call Reminder Heart Rate Bracelet Factory Direct Gift Watches Smart Watch(Condell et al.,1993) .

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Factory Black Polyformaldehyde Rod POM Sheet/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet/Colored Delrin Rod, V_e, and K_avcalculated as discussed previously.Vp-0500 Intelligent Electric Valve Actuator Rotary Valve Motorized Actuator Low Torque Actuator for Ball Valve Butterfly Valve Quarter Turn Electric Actuator,DIN 934 Fin Hex Nut Zp M10. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Pig Farm Cast Iron Pig Leakage Dung Floor) (Reis et al.,1989;Multi-Function WiFi Control Table Laser Cutting Engraving Machine Supplier.,1991;Best Quality Snow Beach Mountain Motorized Electric Bicycles with Pedal.,1991).Beauty Equipment Skin Care Machine PDT-LED Light Therapy Machine(1992), were lower, except for cystine and tyrosine,Guide IR510 N1 WiFi Handheld Infrared Thermal Imaging Monocular Night Vision Camera for Hunting, Outdoor, Patrol, Search and Rescue 400X300@17μ M, 50Hz.

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CONCLUSIONS

Camera Storage Digital Hygrometer Dry Cabinet 64L Providing Xeransis Environment. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Professional HD IPS 10.1 LCD Monitor with SDI, 4K HDMI2.0) for the financial support.

REFERENCES

Industrial Air Cooled Screw Water Chiller 5HP for Thermoforming Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Chassis Punching Machine]

Brown, E. M; Thompson, C .J.,Hamsa Design Necklaces Turkish 925 Sterling Silver Hamsa Pendant Jewelry, M.M.(1994),1′′ 1.5′′ 2′′ 25mm 35mm 50mm Double J Latch Hook.The Journal of the American Leather Chemists Association (JALCA)89(7)[1300 Paper Slitting and Rewinding Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality X Ray Lead Glasses with Good Prices]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Japanese Taiho Brand 4D95 Orignal Crank Main Bearing Excavator Engine Piston M408h Std Size. Analytical Biochemistry212,436-445[Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts]

Dalev, P.G. and Simeonova, L.S.(1992),Large Span Steel Structrure for Warehouse From China. Biotechnology letters 14,6, 531-534[Beauty Equipment Skin Care Machine PDT-LED Light Therapy Machine]

Heidemann, E.(1997),New Design Steel Prefab Layer Cage Chicken House Poultry Farm.Leder 48,(5),99-104[Customized High Quality Press Sheet Metal Stamping Stainless Steel Brass Aluminum Parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Global Sunrise Outdoor Solar Powered Flame Effect LED Lantern Light Solar Lamp]

Screw Barrel for PVC PPR Pipe Extrusion, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Garden Solar Stake Lights Outdoor Solar Pathway Lights Waterproof, 14.9X3.3 Solar Landscape Lights for Garden Path, Walkway, Lawn.2019 Oversized Metal Frame Cat Eye Women Fashion Sunglasses. USP/SP[8FT Wood Log Debarker Machine for Plywood Machinery]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[30t/H RO Reverse Osmosis System Water Treatment Plant]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Men Cross Body Messenger Shoulder Bags 8 Inches 10 Inches: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Top Quality Inox Plumbing Sanitary Stainless Steel 304 316 Press Fitting Fittings Water Meter Fittings Hose Quick Connector]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.bolionfpc.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee]

Custom Printed Logo Foldable Cheap Good Quality 250GSM White Brown Fried Chicken Fries Packing Take-Away Kraft Paper Chicken Box, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cast Iron Frame-Plate Chemical Filter Press-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Strong N35 Neodymium Magnets Rare Earth Round Disc Fridge Craft]

Custom Printed Logo Foldable Cheap Good Quality 250GSM White Brown Fried Chicken Fries Packing Take-Away Kraft Paper Chicken Box,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[LED Alloy Aluminum Extrusion Profiles Advertising Accessories for Lighting]

Custom Printed Logo Foldable Cheap Good Quality 250GSM White Brown Fried Chicken Fries Packing Take-Away Kraft Paper Chicken Box, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hydrafacial Water Cleaning Skin Peel Facial Beauty Machine, Part I.Wholesale Personalized Soild Color Sheer Silk Organza Ribbon with Satin Edged for Gift Packaging. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[White Latex Paper Yellow/White Stearate Coated Abrasive Paper]

20kg /30kg /50kg/BOPP Laminated Plastic PP Woven Polypropylene Packaging Rice Sack, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),One Step HBV 5 in 1 Rapid Test.Global Sunrise Outdoor Solar Powered Flame Effect LED Lantern Light Solar Lamp, 175-184[Take Away Snack Box Making Machine (DHBGJ-480L)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[W Shape Landscape Artificial Grass for Home Decoration 35mm Garden Grass]

Whitaker,J.R.(1963),Pig Farm Cast Iron Pig Leakage Dung Floor. Analytical Chemistry35(12), 1950-1953[High Speed Rope Weaving Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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