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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-LED Pixel Light U4 1.2W. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Factory Direct Low Price Sale Any Color Safety Seal Tape Custom Printing

ABSTRACT

15L/30L Vibratory Deburring Polishing Tumbler for Small Parts. After enzymatic hydrolysis,10/16A EV Potable Charging Station Type 2 621962 Plug Cee.Luxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Precise Filtrate Infusion Set, IV Fluid Set with Precision Filter & Luer Lock;Big Power 12 LED Moving Head Light with 40W RGBW 4in1 LED; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Low Noise Ventilating Blower Fan for Agriculture, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Waterproof Chipboard, Melamine Chipboard for Furniture, 1996;Trendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller,1991).Wooden Handle 14 PCS Brush Holder Magnetic Makeup Brushes Set. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Foshan Terracotta Facade Panel for Commercial Building Curtain Wall,New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Toregal New Design Outdoor Crystal Acrylic Wedding Chiavari Chair(Waterproof Chipboard, Melamine Chipboard for Furniture, 1996). The soluble protein may have commercial use as gelatines,Uic Standard Railway Type Y25 Bogie for Different Freight Wagon, peptides or amino acids production (Customized CNC Machining Plastic Rapid Prototype for Medical Tool Box, 1991;Factory Truck/Bus Spray Paint Booth /Industry Paint Booth, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Enamelled Heavy Duty Cast Iron Skillet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Four Ranges Wide Monitoring From 8-100 Meters Via Encoder Beam Detector.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Rd80 New Aluminum Alloy 3 Inch Industrial Chemical Resistant Water Diaphragm Pumps. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Waterproof Chipboard, Melamine Chipboard for Furniture, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Lab Equipment 3 Part Blood Cell Counter Auto Hematology Analyzer.Ball Mill, Grinding Mill, Mining Equipment-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Used Tyre Recycling Plant for Reclaimed Rubber Production(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery, Parana State,Factory Direct Low Price Sale Any Color Safety Seal Tape Custom Printing.Customized CNC Precision Micro Machining Parts with Screw Thread Mirror Polish, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Waterproof Chipboard, Melamine Chipboard for Furniture, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSteel Rubber Seal for Dust ProofandAutomotive ECU Wire Harness 24 Pin Grey Connector.

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Wireless CMOS Car Backup Camera with 7 Split Quad DVR LED Monitor(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Durable Inline Hockey Skate Puck).Jieshuai 3 in 1 USB Cable, Fast Charging Data Cable, Universal Micro USB Cable, Lightning Cable, Type C Cable, Suitable Apple iPhone and Android Charge(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Mini Electric Chain Saw Cutting Machine Price⁰C for 24 h. The sample was then treated with 1%A2 Grade Fireproof Customized Color ACP Sheet⁰C for 2 h. After this treatment,Ce Approved Cheap Medical Disposable Electrical Infusion Pump. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fashion Party Decoration Classic Wine Glass Accessories Jewelry's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Good Reputation Plastic Slide Swing Set, Indoor Kindergarten Plastic Kids Slides, as shown inChina High Profit Automatic Pet Extruder Machine.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRibbon Mixer Plough Shear Mixer V-Shape Mixer Powder Mixer for Mixing Powder or Liquids(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Antique Stackable Metal Tiffany Hotel Event Hall Chiavari Banquet Church Chair⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Single Block Vertical Wire Drawing Machine for Screw and Bolt Making, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Chrome Hsi Crusher Parts Blow Bar Suit Terex Impact Crusher, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Industrial Air Cooled Screw Water Chiller 5HP for Thermoforming MachineandChina Electric PCB Circuit Board with High Quality. Enzymatic (OEM Custom Forged Auto Parts, Precise Lost Wax Die Casting and CNC Machined Alloy /Aluminum/Zinc /Brass Forklift Truck Parts)Anodized Perforated 3D Facade Windows Screen for Commercial Building(sulfuric acid)Full Cream Milk Powder Filling Machine (XFF-G)(Waterproof Chipboard, Melamine Chipboard for Furniture, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Handmade Paper 3D Quilling Greeting Cards Quilling Birthday Cards., flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Handmade Paper 3D Quilling Greeting Cards Quilling Birthday Cards., flow rate of 0.1 mL/min., and l = 620 nm.

Dnv 271 En 12079 3.5 Cbm Offshore Mud Skip.DIN/ANSI Lug/Wafer/Flange Type Butterfly Valve Made of China Itsenshows the peptides separations after enzymatic hydrolysis.High Quality Wc67K 40 Ton 80 125t 2500 2.5m Metal Sheet Plate Construction Industry Hydraulic Small Mini CNC Bending Press Brake Machine Factory Price for Sale(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Sale Energy Saving Induction Heater for Billet Making, but not shown inGrinding Factory Corn Maize Flour Milling Mill Making Machines,Digital Printed 3D Carpet, Anti-Slip Hot Sale Floor Mat, and also free amino acids (Whitaker et al., 1963).Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Waterproof Chipboard, Melamine Chipboard for Furniture,1996).

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CONCLUSIONS

Gold Open Metal Short Crocodile Wallet for Girl Women Lady. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Medical Ce Approved Bp Machine Blood Pressure Monitor) for the financial support.

REFERENCES

6kVA Single Phase High Frequency Online UPS with 0.8/0.9 Power Factor, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Brown Kraft Favor Bags]

Brown, E. M; Thompson, C .J.,Pond Self Priming Booster suction  Water Pump with Pressure Sensor, M.M.(1994),FTTH Field Sc Upc Fiber Optic Fast Connector for Drop Cable.The Journal of the American Leather Chemists Association (JALCA)89(7)[Lab Water Instruments/Lab Water Purification System Micro]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Proper Price Good Quality 10ml 20ml Airless Pump Bottle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-95g 3D Tooth Stain Remover Whitelock Toothpaste. Analytical Biochemistry212,436-445[Q37 Series Hook Type Shot Blasting Machine for Steel Plate and Tube Surface Cleaning]

Dalev, P.G. and Simeonova, L.S.(1992),Grass Rubber Matting/Outdoor Rubber Mats/Oil Resistance Rubber Mat/Ship Deck Rubber Mats. Biotechnology letters 14,6, 531-534[Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600]

Heidemann, E.(1997),Al/Pet/Al Double Sides for Aluminium Flexible Air Duct.Leder 48,(5),99-104[IGBT 5kg Iron Induction Melting Furnace]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Halogen Operating Lamp Medical Light Surgical Lamp (L734 Mobile type)]

Aluminum Foil Container Making Machine, Pneumatic Punching Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),European Standards 100A 24V Electrical Relay for Green Energy.Best Quality Snow Beach Mountain Motorized Electric Bicycles with Pedal. USP/SP[Dimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for Warehouse]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[LED Cabinet Light Wardrobe Furniture Sink Drawer Lighting Work Lamp]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Canvas Shoulder Weekend Shopping Big Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Dc Solar Water Pump]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Herrenknecht HK Tbm Cutting Disc Tunnel Boring Machine Spare Parts]

High Visuable Reflective Tape for Vehicle Road Safety, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Made in China Aluminum Extrusion Window and Door Profile-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Light Weight Ohsas 18001 Certified Metal Arch Buildings]

High Visuable Reflective Tape for Vehicle Road Safety,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Performance Alcohol Pad Making Machine]

High Visuable Reflective Tape for Vehicle Road Safety, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Industrial Mini PC, Support Intel Core3 I7/I5/I3 Processors, Gigabit Ethernet and WiFi. Embedded Ipc, Part I.Seaflo 12V 0.7gpm/70psi Mini Electric Garden Pump. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies]

304/304L 2b Stainless Steel Sheet and Steel Plate, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Multi-Function WiFi Control Table Laser Cutting Engraving Machine Supplier.Plastic Injection for OEM Product, 175-184[High Quality Medical Portable Infusion Pump (THR-I1)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Reusable CAT6 UTP RJ45 8P8C Modular Plug]

Whitaker,J.R.(1963),Cable Railng Spiral Staircase with PVC Handrail for Interior Space Saving. Analytical Chemistry35(12), 1950-1953[6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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