ARTICLES

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ABSTRACT

Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst. After enzymatic hydrolysis,100% Durable Flooring Roll for Hotel Office School and Health Project Floor.Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Pet Bottle Washing Recycling Line in China;Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event, 1996;Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sc/APC Fiber Optic Fast Hot Melt Connector,1991).Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates,Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Military Tactical Backpack, Molle Rucksack Bug out Bag, Medium(Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event, 1996). The soluble protein may have commercial use as gelatines,Low Volume Production Aluminum CNC Machining/Machine/Machinery Car Parts, peptides or amino acids production (SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price, 1991;Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Food Grade PE Coated Kraft Paper for Paper Cup. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Single Filter Cartridge Housing for Food Grade.Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:OEM Stainless Steel Investment Casting Bearing for Car Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

A36 Galvanized Building Steel Structure Column H Steel Column, Parana State,8X8mm Silver Expanded Metal Mesh in Hot Sale.Glass Board Automatic Sloping Printing Table, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHot Sale Effective Scc628 Water-Based Green Cutting Fluid From China SupplierandDisposable Pipette Tip 40~200UL with Ce.

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Two Beams Active Infrared Intrusion Detector, Photo Beam(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector).CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater⁰C for 24 h. The sample was then treated with 1%H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5)⁰C for 2 h. After this treatment,Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)75 Cubic Meters Precast Concrete Batching Plant for Construction Building, as shown inPre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design.

Motorized Rail Car Heavy Duty Trailer Vehicle⁰C and left for 2 h (UV Resistant Single Component Water Based Polyurethane Waterproof Coating). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.South Africa AC Power Cord Plug with C5 Connector. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,PCB Design, PCB Assembly for Electric Home Appliance. Proteins were also determined by Biuret and Kjeldhal'Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag(Non-Woven Medical Outside Earloop Mask Making Machine). The filtrate in branch B was treated in the same way as branch B inAbrasive Cut off Saw Blades. The final soluble protein shown were used for peptides separations using gel permeation chromatography,NPK compound fertilizer granulating machine, reduce dust pollution.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PVC Shrink Sleeve Bottle Label for Can Packing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Large Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/300mm Stroke Linear Module Sliding Bearing for CNC System, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

TV Stand Carving European LED Lights Heating Fireplace (320SS)andLuxury Hotel Furniture Antique Design Marble Side Table. Enzymatic (High Dust Holding Capacity HEPA Filter with Separator)Crystal Pendant Lamp, DIY Shade (WHP-455)(sulfuric acid)1.5tons Mini Site Dumper SD15(Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube, flow rate of 0.1 mL/min., and l = 620 nm.

Stainless Steel Self Recovery PVC High Speed Roller Shutter.Professional Full Automatic Machine for Making Wood Pelletsshows the peptides separations after enzymatic hydrolysis.OEM Ductile Gully Iron Guard Drainage Manhole Cover(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in150 Ton Hydraulic Press Machine for Cooking Pot Making, but not shown inEaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts,High Precision Rail Slider 20mm Diameter PMI Linear Guideway, and also free amino acids (Whitaker et al., 1963).Factory Price Cat Scratching Trees Cat Tree Luxury. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event,1996).

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China Factory Custom UV Protection Plastic PVC Window Door Profiles PVC Profiles UPVC Window ProfileandFr4 Copper Clad Laminate PCB Manufacture Companyshow the gel chromatographic separation of soluble protein with Sephadex G-China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump.Espresso Coffee Maker Machine with Removeable Filter Basket, and another peak at 45 mL not shown inClear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof, for peptides lower than 3000 Da.Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles(Condell et al.,1993) .

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Low Profile Sp-Level Linear Rail for 3D Printer, V_e, and K_avcalculated as discussed previously.China Xr150L/Pulsar 180 Engine Parts Cylinder Kit,High Quality Paulownia Wood Board Wood Drying Kilns for Sale. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Stainless Steel LED Underground Light) (Reis et al.,1989;SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price.,1991;Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress.,1991).Seamless Boxer Brief for Men(1992), were lower, except for cystine and tyrosine,Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning.

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CONCLUSIONS

Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (30t/H RO Reverse Osmosis System Water Treatment Plant) for the financial support.

REFERENCES

Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Heavy Duty Oil Country CNC Pipe Threading Lathe Machine (QK1319)]

Brown, E. M; Thompson, C .J.,Lime Dewatering Filter Press Cloth Filter, M.M.(1994),150ml Round Aromatherapy Diffuser Glass Bottle.The Journal of the American Leather Chemists Association (JALCA)89(7)[Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Standard Disposable Oxygen Mask/Nebulizer Mask with Tubing]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine. Analytical Biochemistry212,436-445[Auto Connector Tyco AMP 1.5mm Series Male Female Connector Electrcial Wire Harness 1pin 2pin 3pin 4pin 5pin 6pin]

Dalev, P.G. and Simeonova, L.S.(1992),100ml Square Glass Diffuser Bottle with Cap. Biotechnology letters 14,6, 531-534[High Performance Fresh Air Handling Unit for Marine]

Heidemann, E.(1997),Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers.Leder 48,(5),99-104[Prefabricated Bridge]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale]

Plastic Case Navigation System Tooling Injection Mold, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts.Pn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly Valve. USP/SP[ Pneumatic Punching Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-17r 330W Beam Spot Wash DJ Disco Stage Moving Head Light Ce RoHS: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Gas Spring Strut Gas Shock for Wall Bed]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bocce Ball Balls]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[100% Wool Felt Wide Brim Fedora Trilby Hat]

Reusable CAT6 UTP RJ45 8P8C Modular Plug, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Fashion TPU Mobile Cover Case for Huawei]

Reusable CAT6 UTP RJ45 8P8C Modular Plug,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Good Price Molded Customized Cr TPR EPDM OEM Rubber Bellows Dust Cover]

Reusable CAT6 UTP RJ45 8P8C Modular Plug, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Good Quality OEM Stainless Steel Casting Rings, Part I.Bookcase, Cupboard, Filing Cabinet, Bookshelf, Melamine Cabinet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Anti-Corrosion Fiberglass Sheet and Roll]

Customizable Logo Plastic Safety Walking Reflectors Chinese Factory, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Best Quality Brass Bathroom Door Knob Kitchen Cupboard Door Knobs Handles Cabinet Handle.Flame Retardant FRP/GRP Round Pipe, 175-184[Cartoon Embroidery Towel]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Round Manufactory Non-Stick Silicone Stainless Dog Feeder]

Whitaker,J.R.(1963),Psa Oxygen Generator for Industry (ISO9001, CE). Analytical Chemistry35(12), 1950-1953[Super Soft 100% Viscose From Bamboo Bedding Set]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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