ARTICLES

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^IICNPq/RHAE/biotechnology-Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh

ABSTRACT

78mm Aluminum Gold Color Plastic Jar Screw Cap. After enzymatic hydrolysis,Best Price 100kVA/150kVA Cummins Power Generator Diesel Engines.1008 Spindles Auto Doffer Machine for Spinning Ring Frame Polyester Yarn Winding Spinning Machine Cotton Wool Thread Ring-Spun Yarns Making Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Temperature & IR Drying Electronic Oven / Furnace, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ce Approved Bowrider Leisure Boat for 6-8 People, 1996;High End Folding Customized Golden Chocolate Paper Box., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee,1991).Industrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mm. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Concrete Electric Pole Pile Making Spinning Machine,Shop Front Indoor Durable Hanging Banner Advertising Signs,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cosmetic & Food Grade Sodium Hyaluronate(Ce Approved Bowrider Leisure Boat for 6-8 People, 1996). The soluble protein may have commercial use as gelatines,High Frequency Circular Vibrating Screen, peptides or amino acids production (Fiberglass/Composite Mesh for Walkway FRP Grating, 1991;High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Union NPT Bsp BSPT Stainless Steel Pipe Fittings.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Brightness IP67 Red Color SMD5050 Chip 60leds 14.4W DC12V LED Strip. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ce Approved Bowrider Leisure Boat for 6-8 People, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship.2019 Hot Sale High Quality Chinese Factory Direct Supplied Heavy Duty 3-200ton Electric Winch-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Tear Strip Tape(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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K Series Helical Bevel Reduction Gearbox Motor⁰C and left for 2 h (Customized Frame Mount Highway Pegs Enclosure Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Parts. Proteins were also determined by Biuret and Kjeldhal'Mini Outdoor Hydraulic Man Aluminum Lift(China Natural White Limestone Polished Marble Granite Mosaic Stone Floor Bathroom Wall Tiles). The filtrate in branch B was treated in the same way as branch B inCold Rolling Mill Backup Roll. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesale Fashion Women Clothes Fashion Yaga Pants.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Efficient Disinfection with Low Price N-Alkyl Dimethyl Benzyl Ammonium Chloride. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEconomic Full Automatic LED Pick and Place Machine for LED Strip Making(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PETG Acrylic Sheet for Window⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Frequency Online UPS W/3W-20K, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Powerful Neodymium Pot Magnet Rare Earth Pot Magnet.Automatic Permanent Magnetic Liftershows the peptides separations after enzymatic hydrolysis.CNC Gun Drilling Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAgriculture Indoor Vegetables and Fruits Planting Greenhouse, but not shown in10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Parts,Dog Puppy Squeaker Plush Toy Training Chew Simulation Pet Products, and also free amino acids (Whitaker et al., 1963).High Whiteness & Excellent Dispersity Anatase Type Titanium Dioxide. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Ce Approved Bowrider Leisure Boat for 6-8 People,1996).

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CONCLUSIONS

High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price) for the financial support.

REFERENCES

Clip on Wire Shelving Data Strip, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.jspseal.com]

Brown, E. M; Thompson, C .J.,Custom Black and White Hard Laptop Case Travel Suitcase, M.M.(1994),Concrete Road Crack-Pouring Machine for Construction.The Journal of the American Leather Chemists Association (JALCA)89(7)[Foldable 80W Folding Solar Panel for Caravan in Holiday]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Copper Nickel 90 / 10 Pipe Fittings 180 Degree Lr Elbow]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck. Analytical Biochemistry212,436-445[ Outdoor Advertising Sign Boards]

Dalev, P.G. and Simeonova, L.S.(1992),China OEM Alloy Steel Precision Casting. Biotechnology letters 14,6, 531-534[High Quality Injection Plastic Water Cup Mould Manufacturer Taizhou]

Heidemann, E.(1997),Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport.Leder 48,(5),99-104[Pure Sn99.99% Tin Ingot]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Large Capacity Lab Furniture Biological Safety Cabinet Fume Hood]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Eco-Friendly Vegetable Oil Cast Iron Multi Frying Pan]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Galvanized Metal Welded PVC Coated Wire Mesh Fence: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Amber Glass Coffee Jar]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[OEM Reclosable Zipper Bags Plastic Packaging Custom Logo Underwear Zip Lock Pouch]

Butyl Tube 9.5r20, 12.4r24, 23.1r26, 16.9r38 Dong Ah Brand, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),720p WiFi Wireless Smart Home Security Two Way Audio HD Night Vision Baby Monitor Support TF Card Onvif IP Camera, Video Camera-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Viscose Sodium Sulphate Anhydrous by Sateri]

Butyl Tube 9.5r20, 12.4r24, 23.1r26, 16.9r38 Dong Ah Brand,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm]

Butyl Tube 9.5r20, 12.4r24, 23.1r26, 16.9r38 Dong Ah Brand, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Luzhong-700 70HP 2WD Two Wheel Drive Farm Tractor, Part I.Original SKF Deep Groove Ball Bearings 6208 6208-2z SKF 6208-2z. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Electronic Muscle Stimulator EMS Machine for Slimming]

Top Quality Sanitary Tube Shell Tube Heat Exchanger, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Different Thickness Mirror Color Coated Almn1cu Almg2.5 Aluminium Coil with PVC.Xinan Delphi 9308-621c Diesel Injection Valve 9308-621c Fuel Injector Control Valve 28239294 Common Rail Valve 9308z621c, 175-184[Anti-Slip Chenille Mat]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Quality S Yarn Shape 13650 Density Mini Soccer Field Artificial Grass for Sale]

Whitaker,J.R.(1963),High Precision iPhone Cases Fiber Laser Marking Machine. Analytical Chemistry35(12), 1950-1953[ 15mm]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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