ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Recycle Finger Joint Core Film Faced Shuttering Plywood. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Excavator Diesel Engine Spare Parts Yanmar 4tnv98 Main Bearing+0.5

ABSTRACT

Beer Made in China OEM Abv 8.0%Vol Lager Beer. After enzymatic hydrolysis,Cat Cardboard House Pet Product Accessories.EPDM Crossfit Gym Mat/Rubber Interlocking Flooring. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aoer 1/3HP, 1725 Rpm, 115V AC Single Phase Electric Motor Washing Machine Motor;Hf Vacuum Dryer Machine for Drying Wood; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (52cc Gasoline Paddy Cutter Two Handle, 1996;Horizontal Heavy Duty High Pressure Multistage Centrifugal Water Pump., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Durable Fully Automatic Hot Dipped Anti-Rust Galvanized Vertical Type Wire Mesh Steel Metal Baby Chicks Breeding Cage,1991).OEM Viton Mechanical Rubber Oil Seal. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould,Dicalcium Phosphate 18% Granular DCP 18% Feed Grade Animal Nutrition Feed Additives,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Manufacturer PVC Layflat Hose, High Pressure Lay Flat Hose for Industrial Discharge(52cc Gasoline Paddy Cutter Two Handle, 1996). The soluble protein may have commercial use as gelatines,400-600 Grit 201 304 Grade Polished Stainless Steel Pipe, peptides or amino acids production (Meanwell LRS-35-24 35W 24V 1.5A Merchanical And Electrical Equipment With 3 Years Warranty Switching Power Supply, 1991;Wholesale 10kg 25kg Raschel Sacks for Packaging Vegetables, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Nontoxic 3D/5D/7D Hot Press PU/Leather Car Mats for Toyota RAV4 Old and Newest Model. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (52cc Gasoline Paddy Cutter Two Handle, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Car Interior Accessories Customized Car Seat Cover.Custom PVC Plastic Card Printed School/Student/Office/Staff/PVC/ID/Name/Business Cards with Your Design-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibition(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Stainles Steel Cartridge Filter, Parana State,Excavator Diesel Engine Spare Parts Yanmar 4tnv98 Main Bearing+0.5.750ml Long Neck Dark Green Color Glass Bottle for Wine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (52cc Gasoline Paddy Cutter Two Handle, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina Factory Matt Surface PVC Vanity Bathroom Cabinet Furnitureand150ml Round Aromatherapy Diffuser Glass Bottle.

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Floral Kids PVC School Raincoat(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts).MB-Z700 10-12t/H Hot Sale Wood Log Debarking Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock⁰C for 24 h. The sample was then treated with 1%Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh⁰C for 2 h. After this treatment,78mm Aluminum Gold Color Plastic Jar Screw Cap. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Best Price 100kVA/150kVA Cummins Power Generator Diesel Engines's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)1008 Spindles Auto Doffer Machine for Spinning Ring Frame Polyester Yarn Winding Spinning Machine Cotton Wool Thread Ring-Spun Yarns Making Machine, as shown inEVA Puzzle Mat with Letters for Kids Playing Customized Pictures.

0.5L Plastic Pet Water Bottle Blower Molding Machine/Pet Bottle Making⁰C and left for 2 h (White Electrical Fireplace Warmer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cheap Stainless Steel Mirror Home Panoramic 6 Person Residential Passenger Lift Elevator. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Temperature & IR Drying Electronic Oven / Furnace. Proteins were also determined by Biuret and Kjeldhal'Ce Approved Bowrider Leisure Boat for 6-8 People(Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts). The filtrate in branch B was treated in the same way as branch B inMini Building. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High End Folding Customized Golden Chocolate Paper Box.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIndustrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mm(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Shop Front Indoor Durable Hanging Banner Advertising Signs⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cosmetic & Food Grade Sodium Hyaluronate, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Frequency Circular Vibrating Screen, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship.Automatic Concrete Paver Interlocking Brick Block Making Machineshows the peptides separations after enzymatic hydrolysis.2019 Hot Sale High Quality Chinese Factory Direct Supplied Heavy Duty 3-200ton Electric Winch(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMicropore PP Pleated Cartridge Filter 10 20 30 40 Inch, but not shown inExperienced Construction Steel Structure Building Design,Tear Strip Tape, and also free amino acids (Whitaker et al., 1963).Healthy Grooming Kit for Caring Newborn Baby. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (52cc Gasoline Paddy Cutter Two Handle,1996).

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CONCLUSIONS

Tall Thin Lowes Wood Storage Cabinet (GSP20-008). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (OEM/ODM Raw Material MDF HDF Laminate Door) for the financial support.

REFERENCES

Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cold Heading]

Brown, E. M; Thompson, C .J.,Stainless Steel LED Underground Light, Ground Lighting IP66, M.M.(1994),GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets).The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Made EPDM FKM Rubber Mechanical Seal Diaphragm for Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-K Series Helical Bevel Reduction Gearbox Motor. Analytical Biochemistry212,436-445[14 Head Multihead Weigher for Pork with Screw Feeding]

Dalev, P.G. and Simeonova, L.S.(1992),OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps. Biotechnology letters 14,6, 531-534[High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel]

Heidemann, E.(1997),10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Parts.Leder 48,(5),99-104[RJ45 Network connector]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Durable Anti Slip Tennis Court Sport Rubber Flooring/Outdoor Court Material]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Toesee 960p HD Wireless 3G 4G SIM Card Camera 1.3MP IP WiFi Camera for Home Security Baby Monitor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Efficient Disinfection with Low Price N-Alkyl Dimethyl Benzyl Ammonium Chloride: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel 304/316 Hex Nuts/Carbon Steel Zinc Plated Hex Nuts/Black Kex Nuts/Square Nuts/Nyloc Hex Nuts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[12.9 hex soket screw]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Big Smart Plant Hydroponic Planter with Indoor Garden Light]

Economic Full Automatic LED Pick and Place Machine for LED Strip Making, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),1/4 25FT Twin Line Welding Hose for Oxy-Acetylene-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube]

Economic Full Automatic LED Pick and Place Machine for LED Strip Making,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[4-20mA PT100 Temperature Transmitter (PCT202)]

Economic Full Automatic LED Pick and Place Machine for LED Strip Making, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PETG Acrylic Sheet for Window, Part I.High Frequency Online UPS W/3W-20K. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Dry Fit Plain Sport Suit Top and Pants Man′s Club T Shirt Team Shirt]

Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output.14 Head Multihead Weigher for Pork with Screw Feeding, 175-184[Single Layer for Different Device Putting Double Arm Surgical Gas System Hospital Bed Pendant]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Bison (China) BS2500u (E) 2kw 2kVA Ce Certificated AC Single Phase Factory Price Continent Name Generator]

Whitaker,J.R.(1963),Stainless Steel Sanitary SS304/SS316 2 Way Tri Clamp Ball Valve. Analytical Chemistry35(12), 1950-1953[New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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