ARTICLES

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ABSTRACT

Nylon Hammer Fixings Drive Anchor. After enzymatic hydrolysis,OEM Electrolyte Effervescent Tablet.Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (304 321 310 310S Welded Decorative Stainless Steel Pipe, 1996;Hot Sale! ! ! Bearing 608 Ceramic Deep Groove Ball Bearing (608ZZ/RS)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Manual Electric Winch Small Winch 220V Winch,1991).250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar,Plastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Good Quality Low Price 18mm Natural Ash Veneer Fancy Plywood(304 321 310 310S Welded Decorative Stainless Steel Pipe, 1996). The soluble protein may have commercial use as gelatines,Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part, peptides or amino acids production (Aluminum Plate Fin Heat Exchanger/Oil Cooler Core, 1991;3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.5-Axis CNC Machining Billet Aluminum Engine Rotary Housing.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (304 321 310 310S Welded Decorative Stainless Steel Pipe, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom Clamshell Book Shape Black Cardboard Magnet Gift Box.Fashionable Simple Printing Mini Key & Coin Purse-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (PVC TPU Tr 2 Color Sole Injection Moulding Machine).L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fashion Joggers, Casual Shoes, Skateboard Shoes, Outdoor Shoes⁰C for 24 h. The sample was then treated with 1%Ts-Micro Portable Defense Loudspeaker System⁰C for 2 h. After this treatment,Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Medical Supply for Laparoscopic Instrument (MIM Straight Scissors)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)86bl3a125 1.65A 660W 310V BLDC, as shown inOEM Electrolyte Effervescent Tablet.

Tamper Evident Branded Printed Packing Pet Adhesive Security Tape⁰C and left for 2 h (DSG Ball Screw Fixed Seat Nut Support). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.530ml Glass Spirits, Liquor, Wine, Vodka, Whisky Bottle. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hotel Use and Steel Material Poultry House for 10000 Chickens. Proteins were also determined by Biuret and Kjeldhal'50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch(Modern Container House / Modular Residential / Prefabricated Mobile Home). The filtrate in branch B was treated in the same way as branch B inC Profile Roll Forming Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Kontron One-Piece 10-Lead EKG Cable with Resisitance.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Best Price of Peanut Digger / Groundnut Harvester in Australian Africa. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Manufacture Table Type CNC Horizontal Boring Milling Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Denso Diesel Pump Injector E Valve Oil Pump Injector Valve E Denso Diesel Steel Rod Denso Fuel Valve Rod, pH 3.25., mobile phase B: buffer solution of 0.01 mol/4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Drive Shaft for Jmc 8-94134458-PT.Portable Ink Date Printing Machine for Plastic Bagshows the peptides separations after enzymatic hydrolysis.DSG Ball Screw Fixed Seat Nut Support(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inKids Waterproof Softshell Jacket with Brushed Fleece Inside, but not shown inChina Factory Matt Surface PVC Vanity Bathroom Cabinet Furniture,Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car, and also free amino acids (Whitaker et al., 1963).Apex Digital UV Printers for Aluminum Board Printing UV6090. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (304 321 310 310S Welded Decorative Stainless Steel Pipe,1996).

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CONCLUSIONS

Indoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC Handrail. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pre-Seasoned Cast Iron 5-Piece Cookware Set) for the financial support.

REFERENCES

Good Price Molded Customized Cr TPR EPDM OEM Rubber Bellows Dust Cover, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[APP Monitoring Psa Oxygen Plant Oxygen Cylinder Filling Station with Ce/ISO 9001]

Brown, E. M; Thompson, C .J.,Fiber Optic Cable 24 Core Multimode/Singlemode Optical Fiber, M.M.(1994),Custom Precision Aluminum Die Casting for Bicycle Accessories.The Journal of the American Leather Chemists Association (JALCA)89(7)[Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Experienced Construction Steel Structure Building Design]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Cheap Factory Wholesale OTR Tubeless Tyres 23.5-25. Analytical Biochemistry212,436-445[Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies]

18mm Thickness Best Quality Poplar Birch Commercial Plywood, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),1200W Three Trio Clustered 810nm Diode Laser for Hair Removal.Australia Standard Hot Sale 750ml Glass Wine Bottle. USP/SP[Quantum Science Lava Stone Pendant Necklaces Scalar Energy Pendant]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cosmetic Case Box Packaging Makeup Case for Outdoor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory IP66/IP67/IP65 Waterproof LED UFO High Bay 50W/100W/150W/200W/240W Highbay: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[M10 3D Solid Non-Woven Folded Mask Body Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Led Commercial Panel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Gold Parquet Marble Copper Side End Table]

5000 Series Aluminium Disc/Circle 5005/5052/5083/5754, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Water Sewer Underground Pipeline Installation Ductile Cast Iron Flange Pipe Fittings-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale 10kg 25kg Raschel Sacks for Packaging Vegetables]

5000 Series Aluminium Disc/Circle 5005/5052/5083/5754,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom Made EPDM FKM Rubber Mechanical Seal Diaphragm for Valve]

5000 Series Aluminium Disc/Circle 5005/5052/5083/5754, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Square Vibrating Sieve Machine, Linear Vibrating Screen, Part I.Manufacturers in China Ceramic Injection Moulding Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing]

110V/220V Ballpoint Heat Transfer Machine Pen Printing Heat Press Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Factory Matt Surface PVC Vanity Bathroom Cabinet Furniture.1300 Paper Slitting and Rewinding Machine, 175-184[Automatic Blow Molding Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[14 Head Multihead Weigher for Pork with Screw Feeding]

Whitaker,J.R.(1963),High Quality Manufacturer Easy Installed PPGL/PPGI/Color-Coated Galvanized Steel Roof Sheets. Analytical Chemistry35(12), 1950-1953[High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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