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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Composite Plate Dedicated Drop Hose Gear Metering Pump Price, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Brake Fluid Tank Container Hot Plate Plastic Welding Machine, 1996;6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish,1991).Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts,Puffed Core Filling Sanck Production Machine with Ce From China,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting(Brake Fluid Tank Container Hot Plate Plastic Welding Machine, 1996). The soluble protein may have commercial use as gelatines,Cummins Fuel Injector Kit (3076736 3095023), peptides or amino acids production (Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5, 1991;Matrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plastic Therm Forming Filling Sealing Machine, Lid Press Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Electric 100W Three Wheels Toy Car for Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Design Customized 100% Cotton Home Towel Bath Towel. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Brake Fluid Tank Container Hot Plate Plastic Welding Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Toughened Railing Glass.Delrin Sheets with Good Wear Resistance-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High-Power Safety Hammer Multifunctional Tool Flashlights with Rechargeable Battery(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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6082 and 6061 Aluminum CNC Machining Parts for Aircraft(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Electrical Stamping).1054 FRP Pressure Vessel for Water Softener & Water Treatment(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System⁰C for 24 h. The sample was then treated with 1%Non Woven Fabric for Shoe Lining Shoe Inner Good Quality⁰C for 2 h. After this treatment,Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)HDPE Large Diameter Hollowness Wall Winding Pipe Production Line's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Non-Woven Medical Outside Earloop Mask Making Machine, as shown inChina Supplier Dual Voltage Steam Curling Tongs (A129).

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh quality Brass Forged Gate Valve with Casting Iron Handle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cog Mono Round Display LCD Module, pH 3.25., mobile phase B: buffer solution of 0.01 mol/TV Stand Carving European LED Lights Heating Fireplace (320SS), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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OEM ODM Aluminum Casting Parts, V_e, and K_avcalculated as discussed previously.High Pressure Low Volume 2900rpm electric Water Pump,New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Industrial Steel Structure Mezzanine Rack Platform System/Book Shelf) (Reis et al.,1989;Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5.,1991;6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices.,1991).Haivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240)(1992), were lower, except for cystine and tyrosine,Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch.

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CONCLUSIONS

Multifunction Copper Busbar Processing for Aluminum Material. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Plastic Gas and Water End Protective Covers (YZF-H26)) for the financial support.

REFERENCES

Men Genuine Leather Sling Shoulder Satchel Messenger Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Newborn Baby Boys′ Premium Soft Sole Infant Prewalker Toddler Sneaker Shoes Esg11658]

Brown, E. M; Thompson, C .J.,Electric DC 24V Geared Motor for Auto Doors, M.M.(1994),Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided.The Journal of the American Leather Chemists Association (JALCA)89(7)[86bl3a125 1.65A 660W 310V BLDC]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Factort Price Cattle Beef Slaughtering Equipment for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-RF Shielding MRI Door with Brass Frame for RF Cage. Analytical Biochemistry212,436-445[Yashi Smart furniture Smart Side Table]

Dalev, P.G. and Simeonova, L.S.(1992),Geotextile Beg Used for Beach Restoration Geobag. Biotechnology letters 14,6, 531-534[Reusable SS304 316L Stainless Steel Powder 5 10 20 Micron Sintered Filter Cartridge for Indusrial Oil Filter]

Heidemann, E.(1997),Reusable Silicone Food Storage Bag.Leder 48,(5),99-104[Customised Manufacturing Top Quality Children/Baby Clothing Girl Red Wool Coat Winter Clothes Apparel]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hearing Aid Accessories Silicon Straight Tube Curved Tube for Rec and Mic]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fiberglass/Composite Mesh for Walkway FRP Grating]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Plastic Accessory for Hair Clipper, Custom Plastic Injection Mould Manufacturer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Non - Smell Fiber Reinforced PVC Garden Hose]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Nc Slitter Scorer Zero Scoring Corrugated Cardboard Paper Cutting Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ilife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak]

Enig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Dubai Products Rectified Tile for Wall-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hydraulic Hose SAE 100 R1AT]

Enig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Professional High Precision SLS/SLS 3D Printing Action Figure Parts Rapid Prototyping Service]

Enig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:ANSI B16.5 Stainless Steel SS316 Forging Blind Flange, Part I.3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Metal Tin with Clear Lid]

2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Integrated Hydraulic Steering Control Units (BZZ1F SCU).New Model Water Source Heat Pump (SLW100D), 175-184[High Quality Packaging Box with a Small Transparent Part]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Big Mouth 1L Water Drinking Glass Cup Juice Glass Jar Bottle]

Whitaker,J.R.(1963),Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on Garment. Analytical Chemistry35(12), 1950-1953[Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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