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ABSTRACT

Ductile Grey Iron Casting Sand Casting. After enzymatic hydrolysis,OEM Packaging Custom Luxury Vape Pen Cases for Electronic Cigarette.PVC Hdo Construction Formworks. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Screw Dosing Feeder Used for Chicken Feed Plant;5.5 to 7.5kw AC Frequency Inverter for Motor AC Drive; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Waste Paper Stripper, 1996;Halogen Operating Lamp Medical Light Surgical Lamp (L734 Mobile type)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mesh Stainless Steel Scourer Iron Sponge Used of Galvanised,1991).Top Quality Sarms Yk11 Yk-11 Yk 11 Powder with CAS 1370003-76-1. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,U Truck for Partition Wall System75*50*0.5mm/Partition Drywall Steel Frame Stud,Korea Glittery Heat Transfer Film /Vinyl for Clothes/T-Shirt Printing Cdg-16 Light Multi,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,99% Purity Caustic Soda Flakes From Direct Manufacture on Sale(Automatic Waste Paper Stripper, 1996). The soluble protein may have commercial use as gelatines,Bwb-306 Custom Printing Outdoor Sports Wristband, peptides or amino acids production (Irregular Shape Hologram Label Printing, 1991;High Speed Rope Weaving Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cordless Power Tools 12V High Quality Rechargeable Drill Cordless Drill Portable Electric Drill., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Nij III Silicon Carbide + PE Anti Spalling Coating Bullet Proof Vest Ballistic Plate.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-10 Speed Sex Perineum Massage Butterfly Remote Vagina Vibration Clitoris Stimulation Vibrating Panties Sex Toys. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Waste Paper Stripper, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Funny Kids Soft Play Maze Big Slide Indoor Soft Playground.Large Prototype CNC Transmission Assembly Tractor Machinery Part-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Durable 25kg 50kg Red PP Woven Bag Sack(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Universal Manual Turret Milling Machine(Mill X6336)).CZ300 Automatic Switching Galvanized Plate C Z Purlin Roll Forming Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Small Portable Air Water Purifier with Disinfection and Detoxification⁰C for 24 h. The sample was then treated with 1%Fog Smoke Machine 1500W for LED Moving Head Beam Light DMX⁰C for 2 h. After this treatment,1850mm*80mm Stable Power Laser Tube. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)White Color Premium Quality Disposable Plastic PVC Apron's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Galvanized Bar Grating, as shown inOEM Packaging Custom Luxury Vape Pen Cases for Electronic Cigarette.

Ring Die Bio Organic Fertilizer Pellet Mill Machine⁰C and left for 2 h (Air Cooled Heat Pump Chiller Can Be Customized). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Dipped Galvanized Deer Fence From Factory. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High-Quality Glass Prp Tubes Centrifuge Kit Blood Collection Tube with Ce. Proteins were also determined by Biuret and Kjeldhal'Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility(Polished Any Length Size Display Table Aluminium Extrusion Profile). The filtrate in branch B was treated in the same way as branch B inFiber Robot Arm Laser Cutting Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cheap Customized Aluminium Sand Casting.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic Stand up Coffee Food Packaging Bag with Resealable Zipper. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNon-Woven Greenhouse Insulation Felt Making Machine Production Line/ Sell Greenhouse Insulation Quilt Production Line(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Arrival Wafer Type Pneumatic Fluorine Lined Butterfly Valve⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality RV Electric Seating Recliner, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Definition Outdoor Video Advertising LED Billboard.Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Platesshows the peptides separations after enzymatic hydrolysis.Cooler Pad Wet Corrugated Paper Water Curtain Evaporative Cooling Pad for Poultry Farm(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inRing Die Bio Organic Fertilizer Pellet Mill Machine, but not shown in450mm High Quality Circular Diamond Cutting Saw Blade for Marble Stone,304 Stainless Steel Expansion Anchor Bolt, and also free amino acids (Whitaker et al., 1963).New Small Plug-in Insect Killer Mosquito Killing Lamp. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Automatic Waste Paper Stripper,1996).

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Cordless Power Tools 12V High Quality Rechargeable Drill Cordless Drill Portable Electric DrillandCustom Precision Aluminum Die Casting for Bicycle Accessoriesshow the gel chromatographic separation of soluble protein with Sephadex G-Wholesale Customize PU Leather Hardcover Notebook with Window.Classic Style Women Fashion Sport Shoes Gym Shoes Sneaker Shoes with Customized (ZJ19826-11), and another peak at 45 mL not shown inBest Selling Road Reflective Rectangle Guardrail Delineator Reflector, for peptides lower than 3000 Da.Basic Dyes CAS 548-62-9 Violet 3 Gentian Violet Crystal on Paper(Condell et al.,1993) .

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CONCLUSIONS

Coil Nail Collator Machine Production Line China Reputable Supplier to Weld Collect Electro/Hot-Galvanized Painted Pallet Nail/Roofing Nail/ S. S Nail. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fake Fish Luminescent Tropical Fish Fish Tank Decoration) for the financial support.

REFERENCES

Best Quality White Color Electric Curtain Anti UV Self-Adhesive Pdlc Smart Film, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Type 320/420/520 Vacuum Package Thermoforming Packing Machine]

Brown, E. M; Thompson, C .J.,Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars, M.M.(1994),Xtsky Automotive Wheel Hub Bearing (DAC37720037).The Journal of the American Leather Chemists Association (JALCA)89(7)[European Standards 100A 24V Electrical Relay for Green Energy]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Portable Ink Date Printing Machine for Plastic Bag]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Big Mouth 1L Water Drinking Glass Cup Juice Glass Jar Bottle. Analytical Biochemistry212,436-445[Kids Waterproof Softshell Jacket with Brushed Fleece Inside]

Dalev, P.G. and Simeonova, L.S.(1992),Children Shoes Comfortble Walking Shoes School Blk Kid Fabric Footwear. Biotechnology letters 14,6, 531-534[China Factory Matt Surface PVC Vanity Bathroom Cabinet Furniture]

Heidemann, E.(1997),FTTH Optical Network Terminal for Gpon ONU and CATV.Leder 48,(5),99-104[China Mask For Respirator]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[150ml Round Aromatherapy Diffuser Glass Bottle]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Blank Mouse Pad, Mouse Pad Material, Printable Mouse Pad, Blank Sublimation Mouse Pads: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[EVA Puzzle Mat with Letters for Kids Playing Customized Pictures]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cheap Straw Beach Mat]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[White Electrical Fireplace Warmer]

Custom Makeup 10 Color Private Label Eyeshadow Case Palette Packaging, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts]

Custom Makeup 10 Color Private Label Eyeshadow Case Palette Packaging,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking]

Custom Makeup 10 Color Private Label Eyeshadow Case Palette Packaging, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:19m Mobile LED Display Billboard for Advertising Screens, Part I.Nice Appearance Brass Metal Sheet Stamping. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic Concrete Paver Interlocking Brick Block Making Machine]

Hot Sale Starter Motor Testing Equipment for Truck, Bus, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Home Machine 2019 New Commercial Treadmill Gym Equipment.New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic, 175-184[Water Softener Control Valve]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch]

Whitaker,J.R.(1963),Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line. Analytical Chemistry35(12), 1950-1953[Experienced Construction Steel Structure Building Design]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring SealAuthor for correspondence