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ABSTRACT

OEM/ODM Raw Material MDF HDF Laminate Door. After enzymatic hydrolysis,7628 Fiberglass Cloth/Fabric.Stainles Steel Cartridge Filter. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.MB-Z700 10-12t/H Hot Sale Wood Log Debarking Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock, 1996;Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (78mm Aluminum Gold Color Plastic Jar Screw Cap,1991).Best Price 100kVA/150kVA Cummins Power Generator Diesel Engines. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,1008 Spindles Auto Doffer Machine for Spinning Ring Frame Polyester Yarn Winding Spinning Machine Cotton Wool Thread Ring-Spun Yarns Making Machine,0.5L Plastic Pet Water Bottle Blower Molding Machine/Pet Bottle Making,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cheap Stainless Steel Mirror Home Panoramic 6 Person Residential Passenger Lift Elevator(Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock, 1996). The soluble protein may have commercial use as gelatines,High Temperature & IR Drying Electronic Oven / Furnace, peptides or amino acids production (Ce Approved Bowrider Leisure Boat for 6-8 People, 1991;High End Folding Customized Golden Chocolate Paper Box, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Industrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mm.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Concrete Electric Pole Pile Making Spinning Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Shop Front Indoor Durable Hanging Banner Advertising Signs.Cosmetic & Food Grade Sodium Hyaluronate-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Frequency Circular Vibrating Screen(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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2019 Hot Sale High Quality Chinese Factory Direct Supplied Heavy Duty 3-200ton Electric Winch(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Standard Disposable Oxygen Mask/Nebulizer Mask with Tubing).Tear Strip Tape(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Healthy Grooming Kit for Caring Newborn Baby⁰C for 24 h. The sample was then treated with 1%Reusable Stainless Steel Metal Collapsible Portable Foldable Telescopic Straw with Silicone Tips Cleaning Brush⁰C for 2 h. After this treatment,Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Private Label Fitness Wear Sublimation Printing Cheap Long Womens High Waist Yoga Pants Custom's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Emergency Stop Switch 880 Lbs Lift Electric Hoist Crane Remote Control Power System, as shown inAuto Connector Tyco AMP 1.5mm Series Male Female Connector Electrcial Wire Harness 1pin 2pin 3pin 4pin 5pin 6pin.

Auto Valve 4-20mA Flow Control Butterfly Valve⁰C and left for 2 h (High Performance Fresh Air Handling Unit for Marine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Aluminum Street Light Pole. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,WPC Wall Cladding Waterproof Exterior Wall Panels. Proteins were also determined by Biuret and Kjeldhal'Customized Permanent Strong Nickel Block Neodymium Magnet(25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale). The filtrate in branch B was treated in the same way as branch B inLr. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Tall Thin Lowes Wood Storage Cabinet (GSP20-008).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel LED Underground Light, Ground Lighting IP66(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.K Series Helical Bevel Reduction Gearbox Motor⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps, pH 3.25., mobile phase B: buffer solution of 0.01 mol/10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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PETG Acrylic Sheet for Window.Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Partsshows the peptides separations after enzymatic hydrolysis.High Frequency Online UPS W/3W-20K(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Gas Spring Strut Gas Shock for Wall Bed, but not shown in100% Wool Felt Wide Brim Fedora Trilby Hat,Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box, and also free amino acids (Whitaker et al., 1963).Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock,1996).

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CONCLUSIONS

Large Jewelry Display Box for Ring Necklace Earring Storage Case. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine) for the financial support.

REFERENCES

Indoor Tub Customized Shower/Free Standing Shower Enclosure, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Sodium N Cyclohexylsulfamate]

Brown, E. M; Thompson, C .J.,Glow in Dark Ceramic Sublimation Mug 11oz, M.M.(1994),Double Poles Number Bimetal Strip 1AMP Mini Circuit Breaker.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Electric PCB Circuit Board with High Quality]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hamsa Design Necklaces Turkish 925 Sterling Silver Hamsa Pendant Jewelry]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel 201/304/316 CF8/CF8m 1000wog Threaded Ball Valve. Analytical Biochemistry212,436-445[Bitumen Waterproof Membrane Minerla Surface Waterproof Material]

Dalev, P.G. and Simeonova, L.S.(1992),22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine. Biotechnology letters 14,6, 531-534[Centrifugal Casting Stainless Steel Furnace Tube]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Non Woven Fabric for Shoe Lining Shoe Inner Good Quality]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Low Profile Sp-Level Linear Rail for 3D Printer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Clip on Wire Shelving Data Strip: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[FTTH Horizontal 48 Core Fiber Optical Splice Joint Closure]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wfj Series Fine Pulverizer Grinder Unit]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Aluminum Sheet Metal Parts Custom Fabrication]

Custom Black and White Hard Laptop Case Travel Suitcase, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Concrete Road Crack-Pouring Machine for Construction-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Sc/APC Fiber Optic Fast Hot Melt Connector]

Custom Black and White Hard Laptop Case Travel Suitcase,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Bsp 60° Male Cone Seat Carbon Steel Fittings]

Custom Black and White Hard Laptop Case Travel Suitcase, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck, Part I.China OEM Alloy Steel Precision Casting. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting]

Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Best Quality Hose Crimping Machine.1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitor, 175-184[Best Iphone Holder For Car Factory OEM/ODM Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Industrial Low Temperature Water Cooled Scroll Water Chiller]

Whitaker,J.R.(1963),Galvanized Metal Welded PVC Coated Wire Mesh Fence. Analytical Chemistry35(12), 1950-1953[Ribbon Mixer Plough Shear Mixer V-Shape Mixer Powder Mixer for Mixing Powder or Liquids]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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