ARTICLES

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ABSTRACT

86 Type WiFi Smart Touch Switch Controll by Tuya APP. After enzymatic hydrolysis,100% Wool Felt Wide Brim Fedora Trilby Hat.Super Handy Home & Office & Hotel Facial Tissue Paper. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Livestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets, 1996;Transformer Dehydrate Breather Casing (organgle silicon gel)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3 Full HD 1080P Mini Car DVR Dashcam Camera,1991).Food Sanitary Stainless Steel 2000L Fresh Milk Collection Tank. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality X Ray Lead Glasses with Good Prices,Galvanized Steel Track for Sectional Garage Door,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fashion 925 Sterling Silver Pendants Necklace Jewelry(Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets, 1996). The soluble protein may have commercial use as gelatines,Power 4 Leg Transmission Tower From Qingdao Wuxiao Group, peptides or amino acids production (Wholesale Blank Promotional Baseball Cap for Custom Logo Design, 1991;Metal Structural Steel Frame Structure Construction Prefab Warehouse Workshop Factory Storage Building, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Five Axis CNC Flame/Plasma Pipe Cutting and Profiling Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aluminium Alloy Door Frame Profile.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-M16 M20 Stainless Steel Hilti Chemical Thread Bar Anchor Bolt Wedge Hex Bolt Sleeve Anchor Grade 5.6. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality 98% Feed Grade Betaine Anhydrous.Rubber Diaphragm Seals HNBR EPDM Rubber Diaphragm in High Quality-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Unisex Light Weight Quick Dry Sun Protection Sprots Jackets with Hood(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Blince Omsy/Oms Series Hydraulic Orbital Motor Replace Danfoss Oms Motor(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ad-L2 Color Perfume Vial Plastic Bottle5ml 7ml 10ml).Aluminum Sheet Metal Parts Custom Fabrication(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip⁰C for 24 h. The sample was then treated with 1%Blue Color Tie-on Surgical Face Mask Soft Touch⁰C for 2 h. After this treatment,Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ilife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Private Customized High Quality Printed Shipping Labels, as shown inNational Day Holiday Football Bravo Hand Weaving Flag.

DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10⁰C and left for 2 h (Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2019 Latest Design Hot Selling Microfiber Bedding Set Bed Linen. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Product 90GSM Sublimation Paper for Economic Printing. Proteins were also determined by Biuret and Kjeldhal'1.1kw Roof Mounted Variable Speed Water Evaporative Air Cooler for Bolivia Cooling System(Hand Tools 9PCS Hex Key Set CRV Allen Wrench). The filtrate in branch B was treated in the same way as branch B inNew Black Crystal Glass Perfume Bottle Craft for Crystal Gift. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Color Coated Prepainted PPGI PPGL Steel Sheet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pig Equipment for Pig Breeding Farms Rotary Farrowing Crate. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avClear Customized PVC Cosmetic Package Bag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Professional Design Wing Shape Engraved Car Label Emblem⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/38X72X37mm Dac38720037 Wheel Hub  Bearing  for Motor Vehicle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Supplier Dual Voltage Steam Curling Tongs (A129), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Best Selling Road Reflective Rectangle Guardrail Delineator Reflector.High Precision Ce Radial Drill Machine Z3040shows the peptides separations after enzymatic hydrolysis.3-110W Loudspeaker Compression Driver, Neodymium Magnet Tweeter, Gt-7506na(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLuzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor, but not shown in304/304L 2b Stainless Steel Sheet and Steel Plate,PE Pipe in SDR 11 Pressing Rate for Fire-Control, and also free amino acids (Whitaker et al., 1963).Electric Automotive Heater Control Valve. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets,1996).

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Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600, V_e, and K_avcalculated as discussed previously.Stainless Steel Watch,Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve) (Reis et al.,1989;Wholesale Blank Promotional Baseball Cap for Custom Logo Design.,1991;Transformer Dehydrate Breather Casing (organgle silicon gel).,1991).150kw Plastic Bottle Recycling Equipment, Plastic Pet Canister Recycling Machine(1992), were lower, except for cystine and tyrosine,High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine.

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CONCLUSIONS

CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (DIN7505 Cross Recessed Countersunk Flat Head Chipboard Screws) for the financial support.

REFERENCES

Women Luxury Handbags Leather Shoulder Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Chemical Dosing System]

Brown, E. M; Thompson, C .J.,P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop, M.M.(1994),Microfiber Beach Towel Fast Dry Towel, Outdoor Towel, Travel Towel, Swim Towel, Yoga Towel.The Journal of the American Leather Chemists Association (JALCA)89(7)[Large Diameter Aluminum Pipe 3004]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Long Stroke Hydraulic Cylinder for Constraction Machine. Analytical Biochemistry212,436-445[Hydrafacial Water Cleaning Skin Peel Facial Beauty Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Neoprene Protective Elbow Protector Brace Elbow Support (NS0012). Biotechnology letters 14,6, 531-534[Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine]

Heidemann, E.(1997),Big Smart Plant Hydroponic Planter with Indoor Garden Light.Leder 48,(5),99-104[Womens Sports Wear Yoga Pants Splice Leggings Power Stretch Gym Running Workout Tights]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C)]

Disposable Pipette Tip 40~200UL with Ce, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric.High Quality Anti-Corrosion Fiberglass Sheet and Roll. USP/SP[Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Kitchen Floor Mat]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Durable Steel Retainer Plastic Road Safety Trench Cover: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[5.5 to 7.5kw AC Frequency Inverter for Motor AC Drive]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[LED Holiday/Outdoor Colorful Christmas Decoration Fairy String Light 5050]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing]

Industrial Low Temperature Water Cooled Scroll Water Chiller, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fashionable and Hot Selling Women Plastic Sunglasses (WSP8030134)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System]

Industrial Low Temperature Water Cooled Scroll Water Chiller,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Dipped Galvanized Deer Fence From Factory]

Industrial Low Temperature Water Cooled Scroll Water Chiller, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:EV Charger for 120KW DC Fast Electric Car Charging Station, Part I.Custom Velvet Jewelry Box Gift Box with Bowknots for Packing. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Croissant]

Common Style Plastic Wide Tooth Hotel Comb PE Brush Washing Brush, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode.Aluminum Waterproof Folding Marquee Canopy Quick up Tent, 175-184[Punch Breathable Neoprene Patella Strap Knee Support]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[100% Durable Flooring Roll for Hotel Office School and Health Project Floor]

Whitaker,J.R.(1963),4-20mA Current Sensor with Current Meter. Analytical Chemistry35(12), 1950-1953[UL Certified 12V 277VAC Smart Relay for Utility Meters]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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