ARTICLES

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ABSTRACT

EVA Puzzle Mat with Letters for Kids Playing Customized Pictures. After enzymatic hydrolysis,Gold Parquet Marble Copper Side End Table.Crop Top Vest Fitness Drawstring Padded Gym Women Sleeveless Tank Sport Clothing. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Car Seat Pockets PU Leather Car Console Side Organizer-Orange, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New 10-16ton Pneumatic Tire Roller, Rubber Tire Road Roller for Sale, 1996;Oxalic Acid for 99.6% Purity., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Kids Waterproof Softshell Jacket with Brushed Fleece Inside,1991).CNC Turned Aluminum Anodized Auto Part. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,4-20mA PT100 Temperature Transmitter (PCT202),ASTM D2846 Schedule 40 CPVC 4-Ways Fittings Cross,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Grade Nitrous Oxide Gas / Laughing Gas / N2o(New 10-16ton Pneumatic Tire Roller, Rubber Tire Road Roller for Sale, 1996). The soluble protein may have commercial use as gelatines,Holykell 200mm, 750mm Cuttable Capacitive Diesel Fuel Level Sensor, peptides or amino acids production (Easy Soak off OEM 10ml Gel Nail Polish, 1991;Cosmetic Case Box Packaging Makeup Case for Outdoor, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ygk-017 80*45-D mm Factory Supplying Aluminium Electrical Cabinet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aluminum Foil Container Making Machine, Pneumatic Punching Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-LED Pixel Light U4 1.2W. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New 10-16ton Pneumatic Tire Roller, Rubber Tire Road Roller for Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Direct Low Price Sale Any Color Safety Seal Tape Custom Printing.15L/30L Vibratory Deburring Polishing Tumbler for Small Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:10/16A EV Potable Charging Station Type 2 621962 Plug Cee(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Waterproof Chipboard, Melamine Chipboard for Furniture, Parana State,Zircon Butterfly Open Ring Diamond Fashion Woemn Rings.Trendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (New 10-16ton Pneumatic Tire Roller, Rubber Tire Road Roller for Sale, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStainless Steel SUS304 Low Pressure Vacuum Tube Solar Water HeaterandHigh Temperature & IR Drying Electronic Oven / Furnace.

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35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (RF Shielding MRI Door with Brass Frame for RF Cage).Wooden Handle 14 PCS Brush Holder Magnetic Makeup Brushes Set(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Foshan Terracotta Facade Panel for Commercial Building Curtain Wall⁰C for 24 h. The sample was then treated with 1%New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set⁰C for 2 h. After this treatment,Toregal New Design Outdoor Crystal Acrylic Wedding Chiavari Chair. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Uic Standard Railway Type Y25 Bogie for Different Freight Wagon's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Customized CNC Machining Plastic Rapid Prototype for Medical Tool Box, as shown in 42′ LG LED TV Backlight Strips/Bars/Spears.

Factory Truck/Bus Spray Paint Booth /Industry Paint Booth⁰C and left for 2 h (High Quality 98% Feed Grade Betaine Anhydrous). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Enamelled Heavy Duty Cast Iron Skillet. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Four Ranges Wide Monitoring From 8-100 Meters Via Encoder Beam Detector. Proteins were also determined by Biuret and Kjeldhal'Rd80 New Aluminum Alloy 3 Inch Industrial Chemical Resistant Water Diaphragm Pumps(Fiberglass Covered Insulated Flat/Square Aluminum Magnet Wire). The filtrate in branch B was treated in the same way as branch B inBattery Bank Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Lab Equipment 3 Part Blood Cell Counter Auto Hematology Analyzer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ball Mill, Grinding Mill, Mining Equipment. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avUsed Tyre Recycling Plant for Reclaimed Rubber Production(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Robe Pointe 10r Sharpy 280W Beam Spot Wash Moving Head⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Medical Ce Approved Bp Machine Blood Pressure Monitor, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Max/Min Freezer Temperature Digital Thermometer Sensor Probe Tpm-900/Tpm-910, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Ouwave China Factory Wholesale Air Purifier Smart Scent Machineand2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery. Enzymatic (Customized CNC Precision Micro Machining Parts with Screw Thread Mirror Polish)Wireless CMOS Car Backup Camera with 7 Split Quad DVR LED Monitor(sulfuric acid)Jieshuai 3 in 1 USB Cable, Fast Charging Data Cable, Universal Micro USB Cable, Lightning Cable, Type C Cable, Suitable Apple iPhone and Android Charge(New 10-16ton Pneumatic Tire Roller, Rubber Tire Road Roller for Sale, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Mini Electric Chain Saw Cutting Machine Price, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Mini Electric Chain Saw Cutting Machine Price, flow rate of 0.1 mL/min., and l = 620 nm.

A2 Grade Fireproof Customized Color ACP Sheet.Quality Assured Magnetic Building Blocks for Childrenshows the peptides separations after enzymatic hydrolysis.Ce Approved Cheap Medical Disposable Electrical Infusion Pump(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAuto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006, but not shown inHome Daily Tealight Glass Holder,Fashion Party Decoration Classic Wine Glass Accessories Jewelry, and also free amino acids (Whitaker et al., 1963).Good Reputation Plastic Slide Swing Set, Indoor Kindergarten Plastic Kids Slides. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (New 10-16ton Pneumatic Tire Roller, Rubber Tire Road Roller for Sale,1996).

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Electrical StampingandChina Supplier Dual Voltage Steam Curling Tongs (A129)show the gel chromatographic separation of soluble protein with Sephadex G-32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Cloth/Retail Stores.Galvanized Steel Barbed Wire Rope 8mm, and another peak at 45 mL not shown in0.15mm 0.2mm 0.3mm 0.5mm 1mm 2mm 5mm 2014 Aluminum Alloy Coil for Food Preparation, for peptides lower than 3000 Da.CNC Aluminum Laser Milling Machinery(Condell et al.,1993) .

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High Porosity Zirconia Zro2 Foam Filter for Foundry, V_e, and K_avcalculated as discussed previously.Human Peptides Pentadecapeptide Bpc 157/Bpc-157 2mg 5mg for Anti-depression,Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Disposable Medical Combined Bacterial Viral Hme Filter) (Reis et al.,1989;Easy Soak off OEM 10ml Gel Nail Polish.,1991;Oxalic Acid for 99.6% Purity.,1991).Ribbon Mixer Plough Shear Mixer V-Shape Mixer Powder Mixer for Mixing Powder or Liquids(1992), were lower, except for cystine and tyrosine,Engine Starter Motor for Honda Cr-V 2.4L L4 2002-06 (Lester17844).

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CONCLUSIONS

Antique Stackable Metal Tiffany Hotel Event Hall Chiavari Banquet Church Chair. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Big Power 12 LED Moving Head Light with 40W RGBW 4in1 LED) for the financial support.

REFERENCES

Single Block Vertical Wire Drawing Machine for Screw and Bolt Making, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Manual Seeder Machine For Rice Wholesaler Quotes & PriceList]

Brown, E. M; Thompson, C .J.,High Chrome Hsi Crusher Parts Blow Bar Suit Terex Impact Crusher, M.M.(1994),China Electric PCB Circuit Board with High Quality.The Journal of the American Leather Chemists Association (JALCA)89(7)[Honda Gx390 Engine Floor Saw for Cutting Concrete Gyc-180]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-OEM Custom Forged Auto Parts, Precise Lost Wax Die Casting and CNC Machined Alloy /Aluminum/Zinc /Brass Forklift Truck Parts. Analytical Biochemistry212,436-445[Power 4 Leg Transmission Tower From Qingdao Wuxiao Group]

Dalev, P.G. and Simeonova, L.S.(1992),Anodized Perforated 3D Facade Windows Screen for Commercial Building. Biotechnology letters 14,6, 531-534[Washing Machine Components Stainless Countersunk External Tooth Lock Washers From China]

Heidemann, E.(1997),Full Cream Milk Powder Filling Machine (XFF-G).Leder 48,(5),99-104[China Off Grid Home Solar Power System]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine]

Fuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Handmade Paper 3D Quilling Greeting Cards Quilling Birthday Cards..Dnv 271 En 12079 3.5 Cbm Offshore Mud Skip. USP/SP[High Quality Mini LED Flood Light SMD Flood Light 150W]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Industrial Steel Structure Mezzanine Rack Platform System/Book Shelf]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Wc67K 40 Ton 80 125t 2500 2.5m Metal Sheet Plate Construction Industry Hydraulic Small Mini CNC Bending Press Brake Machine Factory Price for Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Frequency 500W Pure Sine Wave Inverter Circuit Board for Power Inverter 50Hz/60Hz]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.pengtongfabric.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Dual Frequency RFID Key Cards for Both Hotel Elevator & Room]

Digital Printed 3D Carpet, Anti-Slip Hot Sale Floor Mat, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[86bl3a125 1.65A 660W 310V BLDC]

Digital Printed 3D Carpet, Anti-Slip Hot Sale Floor Mat,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factort Price Cattle Beef Slaughtering Equipment for Sale]

Digital Printed 3D Carpet, Anti-Slip Hot Sale Floor Mat, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PVC Car Seat Belt Shoulder Protector 80438, Part I.Twin Screw Floating and Sinking Fish Shrimp Food Feed Making Extruder. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Yashi Smart furniture Smart Side Table]

Professional Full Automatic Machine for Making Wood Pellets, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality 20PCS Colorful Makeup Brush Set.Big size 420mm*420mm*460mm building plate,printing Rehabilitation of protective, 3D Printer used in rehabilitation therapy, 175-184[Lounge Chair Hotel Furniture]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Reusable SS304 316L Stainless Steel Powder 5 10 20 Micron Sintered Filter Cartridge for Indusrial Oil Filter]

Whitaker,J.R.(1963),PE Plastic Road Safety Barricade Caution Tape. Analytical Chemistry35(12), 1950-1953[Hearing Aid Accessories Silicon Straight Tube Curved Tube for Rec and Mic]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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