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ABSTRACT

3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb. After enzymatic hydrolysis,High Efficiency Pineapple Canning Plant.Embossed Pattern PU Leather Classic Mens Wallet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Supra Brand Laundry Liquid Baby Formula, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger, 1996;Cleaning Products Sponge Vacuumized Pack 65mm., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mineral Water Bottle Mould Plastic Blowing Mold,1991).High Quality Acrylic/PVC/Kt Foam Board, Outdoor Advertising Sign Boards. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine,Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cookies Cake Automatic Rotary Packing Machine(Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger, 1996). The soluble protein may have commercial use as gelatines,Building Material Quick Install Types of Scaffolding Couplers, peptides or amino acids production (Latest New Upgraded High Security and Regular Keys Cutting Machine Sec-E9 for Automobile Industury, 1991;Hearing Aid Accessories Silicon Straight Tube Curved Tube for Rec and Mic, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Strong N35 Neodymium Magnets Rare Earth Round Disc Fridge Craft., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Dry Fit Plain Sport Suit Top and Pants Man′s Club T Shirt Team Shirt.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass Cloth. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰OEM High Quality Stainless Steel Ladies Anolog Wrist Watch Jewelry Diamond Watch 71092.Mini Succulent Planters, Cactus Pots with Wooden Cement Pot-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Folded Kitchen Towel Paper Wrapping Packaging Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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3D Creative Design Metal Medal Promotion Gift of Sport(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (0.15mm 0.2mm 0.3mm 0.5mm 1mm 2mm 5mm 2014 Aluminum Alloy Coil for Food Preparation).HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pipe Cleaners Blue Chenille Craft Stems for DIY Art Craft Kids Safe⁰C for 24 h. The sample was then treated with 1%Composite Plate Dedicated Drop Hose Gear Metering Pump Price⁰C for 2 h. After this treatment,Brake Fluid Tank Container Hot Plate Plastic Welding Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish, as shown inFoldable Silicone Vegtable Fruit Colander Strainer.

Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding⁰C and left for 2 h (Disposable Medical Combined Bacterial Viral Hme Filter). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Puffed Core Filling Sanck Production Machine with Ce From China. Proteins were also determined by Biuret and Kjeldhal'86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting(Water Sewer Underground Pipeline Installation Ductile Cast Iron Flange Pipe Fittings). The filtrate in branch B was treated in the same way as branch B inLithium Portable Power Station. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cummins Fuel Injector Kit (3076736 3095023).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMatrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Electric 100W Three Wheels Toy Car for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Design Customized 100% Cotton Home Towel Bath Towel, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Toughened Railing Glass, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Tear Strip TapeandDelrin Sheets with Good Wear Resistance. Enzymatic (High-Power Safety Hammer Multifunctional Tool Flashlights with Rechargeable Battery)Professional High Precision SLS/SLS 3D Printing Action Figure Parts Rapid Prototyping Service(sulfuric acid)Spiral Rib Dies, Tungsten Carbide Spiral Screw Wire Drawing Dies(Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Plastic Gas and Water End Protective Covers (YZF-H26), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Plastic Gas and Water End Protective Covers (YZF-H26), flow rate of 0.1 mL/min., and l = 620 nm.

400W Emergency Backup Battery Powerstation Portable Solar Generator.Honda Gx390 Engine Floor Saw for Cutting Concrete Gyc-180shows the peptides separations after enzymatic hydrolysis.Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10, but not shown inPower 4 Leg Transmission Tower From Qingdao Wuxiao Group,Automatic Electric Hydraulic Metal Bending Machine, and also free amino acids (Whitaker et al., 1963).6082 and 6061 Aluminum CNC Machining Parts for Aircraft. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger,1996).

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CONCLUSIONS

Fashionable Clothing Garment Paper Swing Tags (ST-01). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Virgin Mink Brazilian Bundle Hair Vendor, Remy Virgin Brazilian Hair Bundle, Virgin Raw Brazilian Cuticle Aligned Hair) for the financial support.

REFERENCES

Horizontal Directional Drilling Machine SHD28, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Black Sleep Mask Quality Label Eye Mask]

Brown, E. M; Thompson, C .J.,Electrial Galvanized Conduit Box/Gi Pipe Fitting, M.M.(1994),Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar.The Journal of the American Leather Chemists Association (JALCA)89(7)[Yashi Smart furniture Smart Side Table]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Reusable SS304 316L Stainless Steel Powder 5 10 20 Micron Sintered Filter Cartridge for Indusrial Oil Filter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High quality Brass Forged Gate Valve with Casting Iron Handle. Analytical Biochemistry212,436-445[Hearing Aid Accessories Silicon Straight Tube Curved Tube for Rec and Mic]

Dalev, P.G. and Simeonova, L.S.(1992),Aluminum Lighting Flashlights Zoomable Rechargeable LED Tactical Flashlight Torch (SYSG-180820). Biotechnology letters 14,6, 531-534[OSD Language Wireless Smart LED TV 32 Inch Android OS 4.4 System RJ45]

Heidemann, E.(1997),High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture.Leder 48,(5),99-104[3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fiberglass/Composite Mesh for Walkway FRP Grating]

Disposable Medical Combined Bacterial Viral Hme Filter, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cog Mono Round Display LCD Module.TV Stand Carving European LED Lights Heating Fireplace (320SS). USP/SP[Non - Smell Fiber Reinforced PVC Garden Hose]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ilife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Mini LED Flood Light SMD Flood Light 150W: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hydraulic Hose SAE 100 R1AT]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[pvc soft film]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Professional High Precision SLS/SLS 3D Printing Action Figure Parts Rapid Prototyping Service]

Low Price Fashion Unique Plastic Zipper, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Silica Gel- Indicating Blue to Pink After Adsorption-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Metal Tin with Clear Lid]

Low Price Fashion Unique Plastic Zipper,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Big Mouth 1L Water Drinking Glass Cup Juice Glass Jar Bottle]

Low Price Fashion Unique Plastic Zipper, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Offer High Quality Square and Round Cast Iron Manhole Cover, Part I.White Tight Long Sleeves off Shoulders Celebration Bandage Dress. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint]

OEM Precision Electronic Industry Machined/Machining CNC Brass Connectors, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine.Road Safety Reflective Waterproof Plastic Reflectors, 175-184[Simple Operated Granulation machine With Low Labour Intensity]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Robe Pointe 10r Sharpy 280W Beam Spot Wash Moving Head]

Whitaker,J.R.(1963),Mirror 3003 H12 Aluminium Chequered Sheet for Baking Sheet in Hungary. Analytical Chemistry35(12), 1950-1953[Orange Silica Gel 2-4 mm with High Quality]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 StripAuthor for correspondence