ARTICLES

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ABSTRACT

Anodized Perforated 3D Facade Windows Screen for Commercial Building. After enzymatic hydrolysis,Full Cream Milk Powder Filling Machine (XFF-G).Handmade Paper 3D Quilling Greeting Cards Quilling Birthday Cards.. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Dnv 271 En 12079 3.5 Cbm Offshore Mud Skip;High Quality Wc67K 40 Ton 80 125t 2500 2.5m Metal Sheet Plate Construction Industry Hydraulic Small Mini CNC Bending Press Brake Machine Factory Price for Sale; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Digital Printed 3D Carpet, Anti-Slip Hot Sale Floor Mat, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment, 1996;PVC Car Seat Belt Shoulder Protector 80438., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Twin Screw Floating and Sinking Fish Shrimp Food Feed Making Extruder,1991).Professional Full Automatic Machine for Making Wood Pellets. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality 20PCS Colorful Makeup Brush Set,Big size 420mm*420mm*460mm building plate,printing Rehabilitation of protective, 3D Printer used in rehabilitation therapy,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,PE Plastic Road Safety Barricade Caution Tape(Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment, 1996). The soluble protein may have commercial use as gelatines,Screw Barrel for PVC PPR Pipe Extrusion, peptides or amino acids production (15kgs/Spool Aws A5.18 CO2 Gas-Shielded MIG Welding Wire Er70s-6, 1991;Gold Open Metal Short Crocodile Wallet for Girl Women Lady, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;6kVA Single Phase High Frequency Online UPS with 0.8/0.9 Power Factor., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pond Self Priming Booster suction  Water Pump with Pressure Sensor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-FTTH Field Sc Upc Fiber Optic Fast Connector for Drop Cable. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰95g 3D Tooth Stain Remover Whitelock Toothpaste.Grass Rubber Matting/Outdoor Rubber Mats/Oil Resistance Rubber Mat/Ship Deck Rubber Mats-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Al/Pet/Al Double Sides for Aluminium Flexible Air Duct(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Seaflo 12V 0.7gpm/70psi Mini Electric Garden Pump(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump).304/304L 2b Stainless Steel Sheet and Steel Plate(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Multi-Function WiFi Control Table Laser Cutting Engraving Machine Supplier⁰C for 24 h. The sample was then treated with 1%Plastic Injection for OEM Product⁰C for 2 h. After this treatment,Cable Railng Spiral Staircase with PVC Handrail for Interior Space Saving. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Durable Anti Slip Tennis Court Sport Rubber Flooring/Outdoor Court Material's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bison (China) BS2500u (E) 2kw 2kVA Ce Certificated AC Single Phase Factory Price Continent Name Generator, as shown inCosmetic Grade Matte Chromium Green Oxide for Cosmetics.

Experienced Construction Steel Structure Building Design⁰C and left for 2 h (High Speed Mixer for Plastic Extrusion Line). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Heavy Duty 16000L High Pressure Sewer Cleaning Truck. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine. Proteins were also determined by Biuret and Kjeldhal'Non Woven Geotextile Geobag Sadbag Used for Reservoir Lake Dam(Cosmetic & Food Grade Sodium Hyaluronate). The filtrate in branch B was treated in the same way as branch B inVideo Power Extend Cable. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Portable Ink Date Printing Machine for Plastic Bag.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Professional HD IPS 10.1 LCD Monitor with SDI, 4K HDMI2.0. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Frequency 500W Pure Sine Wave Inverter Circuit Board for Power Inverter 50Hz/60Hz(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Best Items Gh/Genatropin with Naked Vial⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Size Gel Nail Polish Set Boxes with Sponge, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Precision Ce Radial Drill Machine Z3040, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Photo BeamandCarbon Fiber Soft TPU Shockproof Back Cover Case for Huawei Honor V10 /Honor V9. Enzymatic (Dual Frequency RFID Key Cards for Both Hotel Elevator & Room)NSF 5 Layers Powder Coated White Kitchen Bathroom Corner Storage Wire Shelf Unit Rack(sulfuric acid)Most Effective Elight+IPL+RF+ND YAG Laser Dermatology Instrument(Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Hot-Sale Products GRP Tower for New Energy Industry, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Hot-Sale Products GRP Tower for New Energy Industry, flow rate of 0.1 mL/min., and l = 620 nm.

Fgg 2b 304 Metal Circular Push Pull Connector.15kgs/Spool Aws A5.18 CO2 Gas-Shielded MIG Welding Wire Er70s-6shows the peptides separations after enzymatic hydrolysis.Thick Wall Large Diameter Aluminium Tube Manufacture(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHandheld Andriod POS with Thermal Printer, but not shown inSeaflo 12V 0.7gpm/70psi Mini Electric Garden Pump,Portable Hydraulic Power Pack 12V Hydraulic Power Unit, and also free amino acids (Whitaker et al., 1963).China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment,1996).

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CONCLUSIONS

AGM Separator for AGM Battery. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Canvas Shoulder Weekend Shopping Big Bag) for the financial support.

REFERENCES

Electric Battery Flameless LED Tea Light, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[bentengcorp]

Brown, E. M; Thompson, C .J.,42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor, M.M.(1994),CE Certificated Wheel Loader (HQ912) with Agricultural Tire.The Journal of the American Leather Chemists Association (JALCA)89(7)[Industrial Packing Paper Pulp Tray Making Machine Egg Tray Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[UV8d Wouxun High Gain Antenna Dual Band Antenna]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Commercial Vegetable Drying Equipment, Yam, Apple Drier with Trays. Analytical Biochemistry212,436-445[Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch]

Dalev, P.G. and Simeonova, L.S.(1992),Cosmetic & Personal Care Grade Xanthan Gum. Biotechnology letters 14,6, 531-534[Rubber Diaphragm Seals HNBR EPDM Rubber Diaphragm in High Quality]

Heidemann, E.(1997),Professiona Ozone Generator for Swimming Pool.Leder 48,(5),99-104[High Pressure Pump for Waterjet Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Luxury Hotel Furniture Antique Design Marble Side Table]

High Chrome Hsi Crusher Parts Blow Bar Suit Terex Impact Crusher, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Precision Plastic Mold Manufacturer in China.Fiberglass Animal Poultry Insulation Grain Tower. USP/SP[Production Line for Making Nonwoven Stiff Waddings and Glue-Free Waddings / Nonwoven Spray Glue Cotton Production Line/ Hot Melt Glue Sheet for Fabrics Laminate]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Trendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Vertical Full Automatic Dark Red Kidney Beans Packing Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar]

New M4 Smart Bracelet Waterproof Bluetooth Step Bracelet Information Call Reminder Heart Rate Bracelet Factory Direct Gift Watches Smart Watch, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Black Polyformaldehyde Rod POM Sheet/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet/Colored Delrin Rod-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Clip on Wire Shelving Data Strip]

New M4 Smart Bracelet Waterproof Bluetooth Step Bracelet Information Call Reminder Heart Rate Bracelet Factory Direct Gift Watches Smart Watch,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Customized Gift Boxing Laser Engraved Zinc Alloy Gold Metal Medal (241)]

New M4 Smart Bracelet Waterproof Bluetooth Step Bracelet Information Call Reminder Heart Rate Bracelet Factory Direct Gift Watches Smart Watch, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Vp-0500 Intelligent Electric Valve Actuator Rotary Valve Motorized Actuator Low Torque Actuator for Ball Valve Butterfly Valve Quarter Turn Electric Actuator, Part I.DIN 934 Fin Hex Nut Zp M10. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[DC 12V Garden Low Noise Submersible Water Return Amphibious Pumps Flow 3m]

Beauty Equipment Skin Care Machine PDT-LED Light Therapy Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Guide IR510 N1 WiFi Handheld Infrared Thermal Imaging Monocular Night Vision Camera for Hunting, Outdoor, Patrol, Search and Rescue 400X300@17μ M, 50Hz.Camera Storage Digital Hygrometer Dry Cabinet 64L Providing Xeransis Environment, 175-184[Gx35 Water Pump]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine]

Whitaker,J.R.(1963),Industrial Air Cooled Screw Water Chiller 5HP for Thermoforming Machine. Analytical Chemistry35(12), 1950-1953[ Neodymium Magnet Tweeter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

304 321 310 310S Welded Decorative Stainless Steel PipeAuthor for correspondence