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ABSTRACT

100% Hand-Painted Canvas Oil Painting Wall Art for Trees. After enzymatic hydrolysis,Factort Price Cattle Beef Slaughtering Equipment for Sale.Supreme 524 Mv Sea Kayak. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Definition Outdoor Video Advertising LED Billboard, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cooler Pad Wet Corrugated Paper Water Curtain Evaporative Cooling Pad for Poultry Farm, 1996;304 Stainless Steel Expansion Anchor Bolt., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Small Plug-in Insect Killer Mosquito Killing Lamp,1991).Wholesale Customize PU Leather Hardcover Notebook with Window. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Classic Style Women Fashion Sport Shoes Gym Shoes Sneaker Shoes with Customized (ZJ19826-11),Basic Dyes CAS 548-62-9 Violet 3 Gentian Violet Crystal on Paper,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,33kv/415V 4000kVA Onan Dyn11 3 Phase Distribution Oil Type Transformer(Cooler Pad Wet Corrugated Paper Water Curtain Evaporative Cooling Pad for Poultry Farm, 1996). The soluble protein may have commercial use as gelatines,China Supplier Non-Slip Anti-Slip Stainless Steel Anti-Slip Crampon Outdoor Walking Hiking Climbing Snow Ice Crampons, peptides or amino acids production (OEM Composite Machining Phenolic Titanium Parts Products Supplier, 1991;DC 12V/24V Coil Voltage 40A General Purpose Power Relay 5pin, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Z511 Custom Logo Clamshell Foam Insert Black Kraft Cardboard Paper Packing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Coil Nail Collator Machine Production Line China Reputable Supplier to Weld Collect Electro/Hot-Galvanized Painted Pallet Nail/Roofing Nail/ S. S Nail.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Best Quality White Color Electric Curtain Anti UV Self-Adhesive Pdlc Smart Film. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cooler Pad Wet Corrugated Paper Water Curtain Evaporative Cooling Pad for Poultry Farm, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars.Xtsky Automotive Wheel Hub Bearing (DAC37720037)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Big Mouth 1L Water Drinking Glass Cup Juice Glass Jar Bottle(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic Bag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Large Capacity Food Processing Cooling Tower Conveyor Equipment).19m Mobile LED Display Billboard for Advertising Screens(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Nice Appearance Brass Metal Sheet Stamping⁰C for 24 h. The sample was then treated with 1%Hot Sale Starter Motor Testing Equipment for Truck, Bus⁰C for 2 h. After this treatment,Home Machine 2019 New Commercial Treadmill Gym Equipment. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line, as shown inAluminum Lamp Body 90mm Cut out Square LED COB Downlights.

Clothing Hard Tag Security Tag for Supermarket/Retail Store⁰C and left for 2 h (Hardcover Bear′s Story Children Book Custom Printing). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.150 Ton Hydraulic Press Machine for Cooking Pot Making. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Mirror Face Style Acrylic Round Wall Mounted Hanging Fish Bowl. Proteins were also determined by Biuret and Kjeldhal'Hot Sale Flat New Design Ladies Winter Warm Shoes(Mineral Water Bottle Mould Plastic Blowing Mold). The filtrate in branch B was treated in the same way as branch B innsen-valve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Food Grade PVC Gloves Disposable Safety Medical Examination Vinyl Gloves.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Factory Plant Manufacturers Hot Feed Rubber Extruder Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.IP67 High-Ending Industrial Socket / Connector⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Mc4 Solar PV Connector TUV Approval, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Bgf-800 Pet Dry Laminating Machine.Best Items Gh/Genatropin with Naked Vialshows the peptides separations after enzymatic hydrolysis.Dimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for Warehouse(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in38X72X37mm Dac38720037 Wheel Hub  Bearing  for Motor Vehicle, but not shown inChina Manufacture Table Type CNC Horizontal Boring Milling Machine,Plastic Bottle Shape Zipper Bag for Candy Snacks Gift Packaging, and also free amino acids (Whitaker et al., 1963).Tec-Sieve Heavy-Duty Expanded Metal Catwalk Grating. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Cooler Pad Wet Corrugated Paper Water Curtain Evaporative Cooling Pad for Poultry Farm,1996).

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High Pressure Pump for Waterjet MachineandHot-Sale Products GRP Tower for New Energy Industryshow the gel chromatographic separation of soluble protein with Sephadex G-New Style PE PVC Carbon Spiral Duct Cable Pipe Extrusion Line / Extruder Machine.Polyester Cocoon Bobbins Thread for Household Textiles, and another peak at 45 mL not shown inHealthy Grooming Kit for Caring Newborn Baby, for peptides lower than 3000 Da.Large Capacity Lab Furniture Biological Safety Cabinet Fume Hood(Condell et al.,1993) .

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HAIRUI 850A+ 600W SMD Rework Soldering Machine, V_e, and K_avcalculated as discussed previously.High Quality Oval Shape Cast Iron Dutch Oven,White Crystal Fashion Bridal Jewelry Accessories Wedding Tiara. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415)) (Reis et al.,1989;OEM Composite Machining Phenolic Titanium Parts Products Supplier.,1991;304 Stainless Steel Expansion Anchor Bolt.,1991).Auto Connector Tyco AMP 1.5mm Series Male Female Connector Electrcial Wire Harness 1pin 2pin 3pin 4pin 5pin 6pin(1992), were lower, except for cystine and tyrosine,Hospital Medical Stainless Steel Top and Base Bedside Locker Jas-109.

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CONCLUSIONS

Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (OEM Customized Polyester Cotton National President Election Supporters Printed Scarf) for the financial support.

REFERENCES

1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Belly Button Ring Jewelry]

Brown, E. M; Thompson, C .J.,High Performance Fresh Air Handling Unit for Marine, M.M.(1994),Community IC Access Card Induction ID Time Card Smart Card.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Sanitary Thread Pneumatic Butterfly Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ 750mm Cuttable Capacitive Diesel Fuel Level Sensor]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Food Packing Machine Biscuit Packing Machine with Auto Feeder. Analytical Biochemistry212,436-445[Wholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Rolamento Agulha Needle Roller Bearing HK1512 HK2020 HK2030 Bearing. Biotechnology letters 14,6, 531-534[100% Polyester Luxury Bedding Comforter Fleece Blanket Throw]

Heidemann, E.(1997),Military Uniform ISO and SGS Standard for Army Camouflage.Leder 48,(5),99-104[High Power LED Wall Mounted Underwater Swimming Pool Light Fitting]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Different Wood Options Rigid Spc Core Vinyl Flooring]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Indoor Tub Customized Shower/Free Standing Shower Enclosure]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Top Quality Inox Plumbing Sanitary Stainless Steel 304 316 Press Fitting Fittings Water Meter Fittings Hose Quick Connector: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Reinforced Rubber Hose]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles]

Used Japanese Mini Excavator Komatsu PC55mr Digger with Rubber Track, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),RV UPS Power 48V 80ah LiFePO4 Battery Pack-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Manufacturer Multi Span Polycarbonate Industrial Green House]

Used Japanese Mini Excavator Komatsu PC55mr Digger with Rubber Track,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Customized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange]

Used Japanese Mini Excavator Komatsu PC55mr Digger with Rubber Track, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Popular Cheap Custom Logo Black Silicone Wristband in High Quality, Part I.Factory Good Quality High Precision Machined Milling CNC Brass Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Luxury Chocolate Color Metal Tin Storage Can Candy Packaging Box]

Volatile Oil, Plant Extracts, Feed Antibiotic Substitutes, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ad-L2 Color Perfume Vial Plastic Bottle5ml 7ml 10ml.5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers, 175-184[Dental Ceramic Brushes Kit]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[20399560 Oil Seal 125X148X9.7 for Volvo]

Whitaker,J.R.(1963),Automatic Single PC Sachet Bag Wet Tissue Machine. Analytical Chemistry35(12), 1950-1953[Fr4 Double Sided PCB Fr4 PCB with Green Solder Mask Custom Printed Circuit Board OEM]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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