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ABSTRACT

Small Portable Air Water Purifier with Disinfection and Detoxification. After enzymatic hydrolysis,Fog Smoke Machine 1500W for LED Moving Head Beam Light DMX.1850mm*80mm Stable Power Laser Tube. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;White Color Premium Quality Disposable Plastic PVC Apron;Galvanized Bar Grating; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ring Die Bio Organic Fertilizer Pellet Mill Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Dipped Galvanized Deer Fence From Factory, 1996;High-Quality Glass Prp Tubes Centrifuge Kit Blood Collection Tube with Ce., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility,1991).Cheap Customized Aluminium Sand Casting. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plastic Stand up Coffee Food Packaging Bag with Resealable Zipper,Non-Woven Greenhouse Insulation Felt Making Machine Production Line/ Sell Greenhouse Insulation Quilt Production Line,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Washing Machine Components Stainless Countersunk External Tooth Lock Washers From China(Hot Dipped Galvanized Deer Fence From Factory, 1996). The soluble protein may have commercial use as gelatines,New Arrival Wafer Type Pneumatic Fluorine Lined Butterfly Valve, peptides or amino acids production (High Quality RV Electric Seating Recliner, 1991;2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;100% Hand-Painted Canvas Oil Painting Wall Art for Trees., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factort Price Cattle Beef Slaughtering Equipment for Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Supreme 524 Mv Sea Kayak. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Dipped Galvanized Deer Fence From Factory, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰API 594 Super Duplex Steel Single Disc Dual Disc Plate Wafer Check Valve.1-40mm Thickness and 1.22m, 1.56m Size PVC Celuka Board Manufacturer for Cabinets-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Definition Outdoor Video Advertising LED Billboard(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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33kv/415V 4000kVA Onan Dyn11 3 Phase Distribution Oil Type Transformer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale).China Supplier Non-Slip Anti-Slip Stainless Steel Anti-Slip Crampon Outdoor Walking Hiking Climbing Snow Ice Crampons(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%OEM Composite Machining Phenolic Titanium Parts Products Supplier⁰C for 24 h. The sample was then treated with 1%DC 12V/24V Coil Voltage 40A General Purpose Power Relay 5pin⁰C for 2 h. After this treatment,Z511 Custom Logo Clamshell Foam Insert Black Kraft Cardboard Paper Packing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Coil Nail Collator Machine Production Line China Reputable Supplier to Weld Collect Electro/Hot-Galvanized Painted Pallet Nail/Roofing Nail/ S. S Nail's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Best Quality White Color Electric Curtain Anti UV Self-Adhesive Pdlc Smart Film, as shown inDisposable Electronic Cigarette 350 Puffs E-Cigarette Multi Flavors Option E-CIGS OEM Package Vape Pen E-Cig.

Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars⁰C and left for 2 h (Portable EMS Shock Wave Massage and Sport Pain Therapy Equipment Beauty Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Xtsky Automotive Wheel Hub Bearing (DAC37720037). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Big Mouth 1L Water Drinking Glass Cup Juice Glass Jar Bottle. Proteins were also determined by Biuret and Kjeldhal'Children Shoes Comfortble Walking Shoes School Blk Kid Fabric Footwear(Hospital Instrument Electric Tower Crane Arm Electric Medical Pendant Model Ecoh62). The filtrate in branch B was treated in the same way as branch B in5ton Front End Loader High Quality Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,FTTH Optical Network Terminal for Gpon ONU and CATV.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM Customized Polyester Cotton National President Election Supporters Printed Scarf. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avD8tc High Performance 3-Electrode Motorcycle Spark Plug(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Makeup 10 Color Private Label Eyeshadow Case Palette Packaging⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic Bag, pH 3.25., mobile phase B: buffer solution of 0.01 mol/19m Mobile LED Display Billboard for Advertising Screens, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

AGM Separator for AGM BatteryandNice Appearance Brass Metal Sheet Stamping. Enzymatic (Hot Sale Starter Motor Testing Equipment for Truck, Bus)Home Machine 2019 New Commercial Treadmill Gym Equipment(sulfuric acid)New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic(Hot Dipped Galvanized Deer Fence From Factory, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line, flow rate of 0.1 mL/min., and l = 620 nm.

Clothing Hard Tag Security Tag for Supermarket/Retail Store.New Design Steel Prefab Layer Cage Chicken House Poultry Farmshows the peptides separations after enzymatic hydrolysis.150 Ton Hydraulic Press Machine for Cooking Pot Making(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in110V-240V LED Filament Bulb Globe Cafe Patio String Light, but not shown inFactory IP66/IP67/IP65 Waterproof LED UFO High Bay 50W/100W/150W/200W/240W Highbay,Mirror Face Style Acrylic Round Wall Mounted Hanging Fish Bowl, and also free amino acids (Whitaker et al., 1963).Hot Sale Flat New Design Ladies Winter Warm Shoes. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Dipped Galvanized Deer Fence From Factory,1996).

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2019 RF Skin Tightening IPL Skin Rejuvenation Shr Hair Removal, V_e, and K_avcalculated as discussed previously.IP67 High-Ending Industrial Socket / Connector,Mc4 Solar PV Connector TUV Approval. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Modern Luxury Hotel Dining Chair with Plated Power Coated Stainless Steel and Fabric) (Reis et al.,1989;High Quality RV Electric Seating Recliner.,1991;High-Quality Glass Prp Tubes Centrifuge Kit Blood Collection Tube with Ce.,1991).PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China(1992), were lower, except for cystine and tyrosine,Reverse Osmosis Water Filtration Device/RO Plant Process/RO Plant Price.

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CONCLUSIONS

Over Band Round Type Electro Magnetic Separator for Tramp Iron. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Small Plug-in Insect Killer Mosquito Killing Lamp) for the financial support.

REFERENCES

Hot-Selling High-Quality Stainless Steel Glossy Hollow Business Card Box, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cold Rolling Molybdenum Foil]

Brown, E. M; Thompson, C .J.,Automatic China Beef Cattle Sheep Tripe Washer Cleaning Machine, M.M.(1994),0.3-30mm Jc-EPE220 EPE Foam Sheet Making Machine Manufacturer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Spiral Rib Dies]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Human Peptides Pentadecapeptide Bpc 157/Bpc-157 2mg 5mg for Anti-depression]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Bgf-800 Pet Dry Laminating Machine. Analytical Biochemistry212,436-445[Layflat Water Delivery Hose Discharge Pipe Pump Agriculture Irrigation Tubing 1inch to 10inch]

Dalev, P.G. and Simeonova, L.S.(1992),Dimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for Warehouse. Biotechnology letters 14,6, 531-534[Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event]

Heidemann, E.(1997),Plastic Bottle Shape Zipper Bag for Candy Snacks Gift Packaging.Leder 48,(5),99-104[China Designer Channel LED Backlit Letter Sign]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Personalized Soild Color Sheer Silk Organza Ribbon with Satin Edged for Gift Packaging]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Luxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Polyester Cocoon Bobbins Thread for Household Textiles: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Aps Replacement Air Compressor Parts (1627410123) Air Oil Separator]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Environmental Test Salt Spray Tester Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Model Water Source Heat Pump (SLW100D)]

Large Capacity Lab Furniture Biological Safety Cabinet Fume Hood, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),HAIRUI 850A+ 600W SMD Rework Soldering Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags]

Large Capacity Lab Furniture Biological Safety Cabinet Fume Hood,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Manual Glass Lifting Machine for Making Double Glass]

Large Capacity Lab Furniture Biological Safety Cabinet Fume Hood, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Oval Shape Cast Iron Dutch Oven, Part I.White Crystal Fashion Bridal Jewelry Accessories Wedding Tiara. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand]

Auto Connector Tyco AMP 1.5mm Series Male Female Connector Electrcial Wire Harness 1pin 2pin 3pin 4pin 5pin 6pin, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hospital Medical Stainless Steel Top and Base Bedside Locker Jas-109.Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch, 175-184[zetindental]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine]

Whitaker,J.R.(1963),1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose. Analytical Chemistry35(12), 1950-1953[Men Genuine Leather Sling Shoulder Satchel Messenger Bag]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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