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ABSTRACT

Wood Design Rigid Core Flooring Spc Click Vinyl Flooring PVC Floor Tile. After enzymatic hydrolysis,Centrifugal Casting Stainless Steel Furnace Tube.Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting;Stainless Steel Automatic Water Flow Control Valve; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Mini Combine, Mini Harvester, Small Harvester Model 4L-1.0, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet, 1996;3phase DC to AC Variable Frequency Drive Water Solar PV Inverter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Quality Printed Cigarette Tipping Paper,1991).White Latex Paper Yellow/White Stearate Coated Abrasive Paper. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Manufacturer Automotive CNC Machined Parts Checking Fixture Components,Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Telescopic Slideway Steel Protection Accordion Cover(Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet, 1996). The soluble protein may have commercial use as gelatines,Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes, peptides or amino acids production (Cosmetic Grade Matte Chromium Green Oxide for Cosmetics, 1991;Ridge Cap Panel Forming Machine Ridge Cap Making Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Grinding Factory Corn Maize Flour Milling Mill Making Machines., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Construction & Decoration ERW Square Steel Tube Manufacturer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet .Household Use Food Packing 8011 Aluminum Foil-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (UV Resistant Single Component Water Based Polyurethane Waterproof Coating).Pet Bottle Washing Recycling Line in China(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale⁰C for 24 h. The sample was then treated with 1%Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings⁰C for 2 h. After this treatment,Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sc/APC Fiber Optic Fast Hot Melt Connector, as shown inNon-Woven Medical Outside Earloop Mask Making Machine.

Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring⁰C and left for 2 h (TV Stand Carving European LED Lights Heating Fireplace (320SS)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302). Proteins were also determined by Biuret and Kjeldhal'Military Tactical Backpack, Molle Rucksack Bug out Bag, Medium(Professional Full Automatic Machine for Making Wood Pellets). The filtrate in branch B was treated in the same way as branch B inwww.newayt.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Low Volume Production Aluminum CNC Machining/Machine/Machinery Car Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAuto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Food Grade PE Coated Kraft Paper for Paper Cup, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Single Filter Cartridge Housing for Food Grade, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

150 Ton Hydraulic Press Machine for Cooking Pot MakingandModern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration. Enzymatic (OEM Stainless Steel Investment Casting Bearing for Car Parts)Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System(sulfuric acid)High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors(Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%30t/H RO Reverse Osmosis System Water Treatment Plant, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%30t/H RO Reverse Osmosis System Water Treatment Plant, flow rate of 0.1 mL/min., and l = 620 nm.

Best Price of Custom Brands Granite Sink for Home.Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Partsshows the peptides separations after enzymatic hydrolysis.A36 Galvanized Building Steel Structure Column H Steel Column(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSBR Round DOT Horse Stall Mat, but not shown inChina Factory Custom UV Protection Plastic PVC Window Door Profiles PVC Profiles UPVC Window Profile,Glass Board Automatic Sloping Printing Table, and also free amino acids (Whitaker et al., 1963).Two Beams Active Infrared Intrusion Detector, Photo Beam. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet,1996).

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CONCLUSIONS

PCB Design, PCB Assembly for Electric Home Appliance. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hy1003 Mixer Shower Set, Luxury Rainfalls Shower Set) for the financial support.

REFERENCES

Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Veneer Peeling Lathe]

Brown, E. M; Thompson, C .J.,NPK compound fertilizer granulating machine, reduce dust pollution, M.M.(1994),PVC Shrink Sleeve Bottle Label for Can Packing.The Journal of the American Leather Chemists Association (JALCA)89(7)[Auto Connector Tyco AMP 1.5mm Series Male Female Connector Electrcial Wire Harness 1pin 2pin 3pin 4pin 5pin 6pin]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Performance Fresh Air Handling Unit for Marine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable. Analytical Biochemistry212,436-445[25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale]

Dalev, P.G. and Simeonova, L.S.(1992),Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch. Biotechnology letters 14,6, 531-534[ Pneumatic Punching Machine]

Heidemann, E.(1997),Large Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2.Leder 48,(5),99-104[10.1 inch display kiosk]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts]

Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),300mm Stroke Linear Module Sliding Bearing for CNC System.PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB. USP/SP[High Quality Gas Spring Strut Gas Shock for Wall Bed]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[100% Wool Felt Wide Brim Fedora Trilby Hat]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Luxury Hotel Furniture Antique Design Marble Side Table: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Fashion TPU Mobile Cover Case for Huawei]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[What Is Titanium Dioxide In Makeup]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Good Price Molded Customized Cr TPR EPDM OEM Rubber Bellows Dust Cover]

High Dust Holding Capacity HEPA Filter with Separator, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Crystal Pendant Lamp, DIY Shade (WHP-455)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Anti-Corrosion Fiberglass Sheet and Roll]

High Dust Holding Capacity HEPA Filter with Separator,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Round Manufactory Non-Stick Silicone Stainless Dog Feeder]

High Dust Holding Capacity HEPA Filter with Separator, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:1.5tons Mini Site Dumper SD15, Part I.1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Super Soft 100% Viscose From Bamboo Bedding Set]

Stainless Steel Self Recovery PVC High Speed Roller Shutter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),OEM Ductile Gully Iron Guard Drainage Manhole Cover.High Precision Rail Slider 20mm Diameter PMI Linear Guideway, 175-184[China Manufacturer Monoblock Hydraulic Directional Control Valve Zdg5]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High End Folding Customized Golden Chocolate Paper Box]

Whitaker,J.R.(1963),Factory Price Cat Scratching Trees Cat Tree Luxury. Analytical Chemistry35(12), 1950-1953[New 14 Inch Diamond Saw Blade for Granite Cutting]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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