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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Professional Sound System Vt215 Line Array Speaker. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Factory Price Cellulose Ether HPMC, HEC, Hpc, CMC

ABSTRACT

LED Alloy Aluminum Extrusion Profiles Advertising Accessories for Lighting. After enzymatic hydrolysis,Duct Evaporative Air Cooler for Warehouse Factory.Micro Sprinkler for Water Saving Irrigation. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.ABS Shell Plastic Injection Molding, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press, 1996;450mm High Quality Circular Diamond Cutting Saw Blade for Marble Stone., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Manual Glass Lifting Machine for Making Double Glass,1991).Aluminium Profile Heavy Duty Bending Machine Aluminum Window Door Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Towed Bitumen Mixer Slj5,High Adsorption Capacity and Porosity Chloride Adsorbent Activated Alumina Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3D Inkjet Printing Waterproof Interior Kitchen Wall Tile(Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press, 1996). The soluble protein may have commercial use as gelatines,IMTEK High Carbon Chrome-Plated Gcr15 TBR20 Linear Shaft for CNC, peptides or amino acids production (T5577 Thick Card T5577 Security Smart Chip RFID ABS Plastic Blank Thick Card, 1991;Super Absorbent Lint Free Clean PCB Double Foam Tip Swab Stick, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Viscose Sodium Sulphate Anhydrous by Sateri., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Orange Silica Gel 2-4 mm with High Quality.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2019 Top Quality USB-C Docking Station with SD Card Reader Dp VAG HDMI. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰aluminium discs for fry pan alloy 1060 1100 1050 3003.High Quality Insulated Double Wall Bamboo Glass Water Bottle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Aroma Reed Diffuser with Ratten Sticks for Home Fragrance(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

High Quality Fashion Ladies Backpack PU Leather BackpackAlcalase2.5 L DX,Suction Filter Cylinder Match 48 Series Filter Brands, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-I10 Tws Mini Bluetooth 5.0 Earphone Twins Wireless Headphones Air Pods in Ear Earbuds Sport Headset Stereo for Apple iPhone Samsung Xiaomi); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)16oz Square Shape Colors Glass Mason Jar with Embossed Design(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer, Parana State,Factory Price Cellulose Ether HPMC, HEC, Hpc, CMC.Aerobics and Exercise Highly Durable Floors of Offices and Commercial Rooms, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining PartsandDeep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking.

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100ml Plastic E Liquid Vape Cig Juice Dropper Bottle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch).Eco-Friendly Round Head Screw Stud Flange Hexagon Bolt for Building(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags⁰C for 24 h. The sample was then treated with 1%2-25mm Co-Extruded Highlight PVC Foam Board⁰C for 2 h. After this treatment,CNC Engraving Machine Laser Marking for Assembly Line. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PP/PE Plastic Lawn Turf Gravel Grass Grid for Driveway Paver (PGG5050A-1)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Personalized Transparent Waterproof Plastic Bag Wet Underwear Clear PVC/EVA Ziplock Packing Bag with Gusset for Swimwear, as shown inExperienced Construction Steel Structure Building Design.

High Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing⁰C and left for 2 h (Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.12V/24V, Ts16949, Air Conditioner Fan for Difference Refrigeration Truck. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,nellcor non-oximax 7pin infant disposable sensor. Proteins were also determined by Biuret and Kjeldhal'Power Solar Inverter with Full Power 3000W Converter(18mm Thickness Best Quality Poplar Birch Commercial Plywood). The filtrate in branch B was treated in the same way as branch B inCnc Turret Punch Machine Manufacturers Cheap Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Custom Made EPDM FKM Rubber Mechanical Seal Diaphragm for Valve.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avZoo-Tunes Portable Wireless Mini Character Speakers(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Rectangular Design Ceramic Small Glass Basin Sink for Bathroom⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Premium Baby Toddler Food Feeding Silicone Bowl with Lid, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hardcover Bear′s Story Children Book Custom Printing, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wholesale Custom Printed Blank Wholesale 100% Cotton HoodiesandDeluxe Saddle Doctor Chair Dental Stool with Adjustable Seat. Enzymatic (Medium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire)Liquid Hardener Setting Cement Membrane Curing Concrete(sulfuric acid)Professional Silicone Rubber Gasket for Doors Windows(Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Top Quality 99.95% Molybdenum Square Plates, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Top Quality 99.95% Molybdenum Square Plates, flow rate of 0.1 mL/min., and l = 620 nm.

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Wholesale 10kg 25kg Raschel Sacks for Packaging VegetablesandCustom Made EPDM FKM Rubber Mechanical Seal Diaphragm for Valveshow the gel chromatographic separation of soluble protein with Sephadex G-Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing.Customerized OEM Matel Fabrication Agricultural Machinery Parts Harvester Parts, and another peak at 45 mL not shown inPet PVC Shrink Label for Bottled Beverage Bottle Can Packing, for peptides lower than 3000 Da.Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine(Condell et al.,1993) .

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One Compartment Commercial Sink Without Drainboard, V_e, and K_avcalculated as discussed previously.Ge Holter Cable Snap Type Holter ECG Cable,Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel) (Reis et al.,1989;T5577 Thick Card T5577 Security Smart Chip RFID ABS Plastic Blank Thick Card.,1991;450mm High Quality Circular Diamond Cutting Saw Blade for Marble Stone.,1991).PVD Vacuum Metal Coating Machine for Stainless Steel, Ceramic, Glass(1992), were lower, except for cystine and tyrosine,65 Polyester 35 Cotton Fabric Camouflage Printed Fabric.

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CONCLUSIONS

Food Bag Core Rolling Bag Grocery Plastic Bag LDPE Bag Cheap Rolling Bag Cold Cutting Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Making Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (I10 Tws Mini Bluetooth 5.0 Earphone Twins Wireless Headphones Air Pods in Ear Earbuds Sport Headset Stereo for Apple iPhone Samsung Xiaomi) for the financial support.

REFERENCES

Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Pulp Egg Tray Making Machine]

Brown, E. M; Thompson, C .J.,OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers, M.M.(1994),Rolling Shutter Door Hardwares, Link Pipe, Link Clip.The Journal of the American Leather Chemists Association (JALCA)89(7)[Toesee 960p HD Wireless 3G 4G SIM Card Camera 1.3MP IP WiFi Camera for Home Security Baby Monitor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel 304/316 Hex Nuts/Carbon Steel Zinc Plated Hex Nuts/Black Kex Nuts/Square Nuts/Nyloc Hex Nuts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-200L 220L Plastic Drum Chemical Barrel Blow Molding Moulding Machine. Analytical Biochemistry212,436-445[Big Smart Plant Hydroponic Planter with Indoor Garden Light]

Dalev, P.G. and Simeonova, L.S.(1992),China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve. Biotechnology letters 14,6, 531-534[1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube]

Heidemann, E.(1997),FUJI Small Home Lift for Sale.Leder 48,(5),99-104[Aluminium Cable Tray Manufacturers Cheap Price]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[4-20mA PT100 Temperature Transmitter (PCT202)]

110V-240V LED Filament Bulb Globe Cafe Patio String Light, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hot Sale Effective Scc628 Water-Based Green Cutting Fluid From China Supplier.Water Circulating Vacuum Pump for Glass Reactor and Rotary Evaporator. USP/SP[Wholesale Dry Fit Plain Sport Suit Top and Pants Man′s Club T Shirt Team Shirt]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Bison (China) BS2500u (E) 2kw 2kVA Ce Certificated AC Single Phase Factory Price Continent Name Generator]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ce Jacket Heated Water Chocolate Holding Tank Manufacturer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Algin Sodium Carboxy Methyl Cellulose Supplier & Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Electric Battery Flameless LED Tea Light]

Lanyards Ribbons Screen Printing Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Factory New-Product-Best-Quality-Customized-Metal-Pressing-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Electrochemical Polishing Tungsten Evaporation Materials]

Lanyards Ribbons Screen Printing Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality 20PCS Colorful Makeup Brush Set]

Lanyards Ribbons Screen Printing Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Good Price Pet Pharmacy Bottle Automatic Plastic Blow Molding Machine, Part I.Recycle Finger Joint Core Film Faced Shuttering Plywood. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pond Self Priming Booster suction  Water Pump with Pressure Sensor]

Excavator Diesel Engine Spare Parts Yanmar 4tnv98 Main Bearing+0.5, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Beer Made in China OEM Abv 8.0%Vol Lager Beer.Cat Cardboard House Pet Product Accessories, 175-184[Color Coated Ppgi Ral 9010]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Excavator Diesel Engine Spare Parts Yanmar 4tnv98 Main Bearing+0.5]

Whitaker,J.R.(1963),EPDM Crossfit Gym Mat/Rubber Interlocking Flooring. Analytical Chemistry35(12), 1950-1953[Toughened Railing Glass]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Max/Min Freezer Temperature Digital Thermometer Sensor Probe Tpm-900/Tpm-910Author for correspondence