ARTICLES

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^IICNPq/RHAE/biotechnology-Ouwave China Factory Wholesale Air Purifier Smart Scent Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Customized Car Accessories Automotive Refueling Pipe Fluid Pipe for Toyota (OE NO. 77201-44161)

ABSTRACT

Different Wood Options Rigid Spc Core Vinyl Flooring. After enzymatic hydrolysis,High Quality Bucket Segment Bolt 5p7665.Simple Hot Sell Wooden Watch Quartz Wristwatch Custom Logo Wholesales. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Straight Seam Galvanized Pipe High Frequency Tube Welding Mill;China New Side Opening Hydraulic Crimping Machine for Car Aircondition Hose; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Flow Packing Machine for Bread, Soap, Biscuit, Chocolate, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal, 1996;Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer,1991).Outdoor LED Decorative Street Linear Wall Lighting. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Good Quanlity of Ue319an20z Hydraulic Oil Filter Element,Universal Manual Turret Milling Machine(Mill X6336),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pod Style Doval Vape Pen battery 170mAh Vape Pod 0.5ml Electronic Cigarette Without E Liquid Vape Batteries(Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal, 1996). The soluble protein may have commercial use as gelatines,Hexagonal Netting/ Gabion Box/ Gabion Basket, peptides or amino acids production (Proper Price Good Quality 10ml 20ml Airless Pump Bottle, 1991;BSCI Audit Custom 100% Polyester Embroidered Trucker Cap Bottle Opener, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fire Retardant Reflective Aluminium Foil Woven for Insulation Material., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fireproof WPC Indoor Decorative Wall Panel.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Layflat Water Delivery Hose Discharge Pipe Pump Agriculture Irrigation Tubing 1inch to 10inch. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰3D Decorative Soundproof Panel Polyester Fiber Acoustic Panel for Interior Wall.High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CNC Machinery Aluminum and ABS Mock up Prototypes(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Wholesale Six Patterns for Light 6in1 Sale Laser Grating Lens and Mirrors, Parana State,Customized Car Accessories Automotive Refueling Pipe Fluid Pipe for Toyota (OE NO. 77201-44161).Professional Melting (30kg-30000kg) Scrap Induction Heating Furnace for Iron/Steel/Gold/Silver/Aluminium etc, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGlass Board Automatic Sloping Printing TableandMedical Supply for Laparoscopic Instrument (MIM Straight Scissors).

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Corrugated Paper Honeycomb Core Board for Doors(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (99% Purity Caustic Soda Flakes From Direct Manufacture on Sale).Home Depot Electric Fireplace with Deco Insert Gas Heaters Fan Modern Furniture(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wood Handle Carbon Steel Sickle S202⁰C for 24 h. The sample was then treated with 1%High Quality Custom Golden Colored Powder Pill Organizer⁰C for 2 h. After this treatment,Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Disposable Pipette Tip 40~200UL with Ce's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale, as shown in150kw Plastic Bottle Recycling Equipment.

1.5 Oz Mini Hexagon Glass Jars with White Plastisol Lined Lids⁰C and left for 2 h (Flex Cable FPC Tab Cof Hot Pressing Acf LCD Bonding Machine for TV Repair Tools). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.86 Type WiFi Smart Touch Switch Controll by Tuya APP. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,100% Wool Felt Wide Brim Fedora Trilby Hat. Proteins were also determined by Biuret and Kjeldhal'Super Handy Home & Office & Hotel Facial Tissue Paper(4mm 6mm2 Soler Extension Leads with Mc4). The filtrate in branch B was treated in the same way as branch B inMRI Doors. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hidden Handle Safe for Children in Bathroom Cabinet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLivestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Transformer Dehydrate Breather Casing (organgle silicon gel)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/3 Full HD 1080P Mini Car DVR Dashcam Camera, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Food Sanitary Stainless Steel 2000L Fresh Milk Collection Tank, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Metal Structural Steel Frame Structure Construction Prefab Warehouse Workshop Factory Storage Building.Cast Iron Frame-Plate Chemical Filter Pressshows the peptides separations after enzymatic hydrolysis.Five Axis CNC Flame/Plasma Pipe Cutting and Profiling Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEnig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill, but not shown inWater Circulating Vacuum Pump for Glass Reactor and Rotary Evaporator,Aluminium Alloy Door Frame Profile, and also free amino acids (Whitaker et al., 1963).M16 M20 Stainless Steel Hilti Chemical Thread Bar Anchor Bolt Wedge Hex Bolt Sleeve Anchor Grade 5.6. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal,1996).

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Professional Pulse Dust Filter for Grain Processing, V_e, and K_avcalculated as discussed previously.Plug-in Assembly Type Straight Through Joint and Insulation Joint,DIN7505 Cross Recessed Countersunk Flat Head Chipboard Screws. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car) (Reis et al.,1989;Proper Price Good Quality 10ml 20ml Airless Pump Bottle.,1991;Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C).,1991).Fog Light LED Light Bar Wire Harness with LED Light on off Waterproof Toggle Switch Wire Assembly(1992), were lower, except for cystine and tyrosine,High Speed Automatic Slitting Machine for Plastic Film.

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CONCLUSIONS

China Natural White Limestone Polished Marble Granite Mosaic Stone Floor Bathroom Wall Tiles. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Plastic Nylon Sheet with High Temperature) for the financial support.

REFERENCES

Blince Omsy/Oms Series Hydraulic Orbital Motor Replace Danfoss Oms Motor, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bbq Set High Quality Supplier]

Brown, E. M; Thompson, C .J.,Aluminum Sheet Metal Parts Custom Fabrication, M.M.(1994),Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip.The Journal of the American Leather Chemists Association (JALCA)89(7)[NBR EPDM Neoprene Silicone Rubber Sheet for Seal]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Haivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Blue Color Tie-on Surgical Face Mask Soft Touch. Analytical Biochemistry212,436-445[Large Soft Cooler Bag Insulated Lunch Box Bag Picnic Cooler Tote with Dispensing Lid]

Dalev, P.G. and Simeonova, L.S.(1992),Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine. Biotechnology letters 14,6, 531-534[Durable Steel Retainer Plastic Road Safety Trench Cover]

Heidemann, E.(1997),Ilife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak.Leder 48,(5),99-104[Customized Polishing Single Cavity Plastic Injection Mould Auto Parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Precision Rail Slider 20mm Diameter PMI Linear Guideway]

Metal Tin with Clear Lid, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Private Customized High Quality Printed Shipping Labels.DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10. USP/SP[Pig Equipment for Pig Breeding Farms Rotary Farrowing Crate]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mirror Face Style Acrylic Round Wall Mounted Hanging Fish Bowl]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2019 Latest Design Hot Selling Microfiber Bedding Set Bed Linen: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ IV Fluid Set with Precision Filter & Luer Lock]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Prefabricated H Beam Steel Structure Building]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Professional Sound System Vt215 Line Array Speaker]

New Product 90GSM Sublimation Paper for Economic Printing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),1.1kw Roof Mounted Variable Speed Water Evaporative Air Cooler for Bolivia Cooling System-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Logo Printing Cheap Gold Custom Paper Clip]

New Product 90GSM Sublimation Paper for Economic Printing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[EPE Foam Package Extruding Production Line]

New Product 90GSM Sublimation Paper for Economic Printing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Color Coated Prepainted PPGI PPGL Steel Sheet, Part I.Pig Equipment for Pig Breeding Farms Rotary Farrowing Crate. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass Cloth]

Clear Customized PVC Cosmetic Package Bag, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Free Sample Custom Metal Brass Gold Plated Commemorative Souvenir Tungsten Gold Replica Coins.Factory Professional Design Wing Shape Engraved Car Label Emblem, 175-184[egbadges.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Outdoor P5mm Programmable Bus LED Display Sign]

Whitaker,J.R.(1963),38X72X37mm Dac38720037 Wheel Hub  Bearing  for Motor Vehicle. Analytical Chemistry35(12), 1950-1953[Eco-Friendly Round Head Screw Stud Flange Hexagon Bolt for Building]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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