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ABSTRACT

500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory. After enzymatic hydrolysis,Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen.New Style Indoor Dynamic Digital LED Waterfall Water Curtain Fountain. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;New 14 Inch Diamond Saw Blade for Granite Cutting;TV Antenna (CH50006); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.4PCS High Quality Adjustable Wrench Set (FST-41), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Three F Female Connector Adapter, 1996;Tongkai Classic Business Hardcover Leather Notebook., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Customization! Bdy380CD Horizontal Directional Drilling Machine,1991).500kg Capacity 14m Height Semi Electric Scissor Lift. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Quality S Yarn Shape 13650 Density Mini Soccer Field Artificial Grass for Sale,DIN/ANSI Lug/Wafer/Flange Type Butterfly Valve Made of China Itsen,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,SAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose(Three F Female Connector Adapter, 1996). The soluble protein may have commercial use as gelatines,Small Batch Processing Football Net, peptides or amino acids production (11AC 1200m Dual-Band Wireless Whole Home Mesh WiFi System WiFi Mesh Router, 1991;New Condition Textured Peas Soy Protein Making Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale Tractor Engine Spare Parts Injector Nozzle for Mf 240 OEM No Dll 150s6556., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-SBR Round DOT Horse Stall Mat, Rubber Stable Mat. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Three F Female Connector Adapter, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Customized Frame Mount Highway Pegs Enclosure Parts.Double Wine Carrier Bag Gift Box Tote-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:IP44 Waterproof 3pin 16A Electrical Male and Female Industrial Plug and Socket(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Embossed Lattice PU Synthetic Leather for Bags/Handbags (HS703)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen).Physical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Standard Disposable Oxygen Mask/Nebulizer Mask with Tubing⁰C for 24 h. The sample was then treated with 1%Extruder Floating/Sinking Fish Feed Pellet Dryer⁰C for 2 h. After this treatment,Custom Durable Silicone Rubber Seal Ring for Pressure Cooker. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Kunba Vertical Automatic Food Packing Sealing Machine CBS-1100V, as shown inSmiley Canvas School Backpack for Teenagers Girls.

Quality Assured Magnetic Building Blocks for Children⁰C and left for 2 h (Canvas Shoulder Weekend Shopping Big Bag). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary. Proteins were also determined by Biuret and Kjeldhal'Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine(Liquid Hardener Setting Cement Membrane Curing Concrete). The filtrate in branch B was treated in the same way as branch B inSc Fiber Optic Fast Connector for Patch Cord. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cummins L10 Engine Piston Cooling Nozzle 3070218. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAccess control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.SMT E2 Type Air Coils Inductors Inductance Range 5.0∽ 60.0nh E2r230-10.3nj for Ssb Radio Equipment Use⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/99% Al2O3 High Alumina Ceramic Ball, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Price Long Life Portable Peltier Air Cooler, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Stainless Steel French Earring Hook for Jewelry.Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5shows the peptides separations after enzymatic hydrolysis.Professional Metal Stamping Parts with Anodizing(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Grade Nitrous Oxide Gas / Laughing Gas / N2o, but not shown inWhite Color Premium Quality Disposable Plastic PVC Apron,Agriculture Indoor Vegetables and Fruits Planting Greenhouse, and also free amino acids (Whitaker et al., 1963).Night Vision Wide HD Waterproof Car Side View Camera for Bus. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Three F Female Connector Adapter,1996).

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CONCLUSIONS

D405 Trio 300W 4 Voice Coil Selemium Driver De-1001. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Gt4b63 Can Seamer Machine) for the financial support.

REFERENCES

150 micron steel mesh filter magnetic hydraulic filter cartridge, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Heat Fans for Smoke Extraction System]

Brown, E. M; Thompson, C .J.,Fuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711, M.M.(1994),Wholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fashionable Simple Printing Mini Key & Coin Purse]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Top Stainless Steel Baluster Designs Stair Baluster Railing Handrail]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Zircon Butterfly Open Ring Diamond Fashion Woemn Rings. Analytical Biochemistry212,436-445[Stainless Steel Automatic Water Flow Control Valve]

Dalev, P.G. and Simeonova, L.S.(1992),EVA Puzzle Mat with Letters for Kids Playing Customized Pictures. Biotechnology letters 14,6, 531-534[Concrete Electric Pole Pile Making Spinning Machine]

Heidemann, E.(1997),Gold Parquet Marble Copper Side End Table.Leder 48,(5),99-104[High Pressure Oil Resistant High Pressure Hydraulic Hose R1 R2]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Used Tyre Recycling Plant for Reclaimed Rubber Production]

Boiler Room Ventilation Exhaust Fan, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Crop Top Vest Fitness Drawstring Padded Gym Women Sleeveless Tank Sport Clothing.Foldable 80W Folding Solar Panel for Caravan in Holiday. USP/SP[Children Shoulder Backpack Bag Leisure School Bag]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ 16.9r38 Dong Ah Brand]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Silver Anodized Aluminium 135 Degree Angle Bracket: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wholesale Scarf Customize Logo Sports of Printing Scarf Football Scarf Fan Scarf (C1033)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Makeup 10 Color Private Label Eyeshadow Case Palette Packaging]

Car Seat Pockets PU Leather Car Console Side Organizer-Orange, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New 10-16ton Pneumatic Tire Roller, Rubber Tire Road Roller for Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesalev Custom Breathable Polyester Fabric Slim Fit Gym Singlet]

Car Seat Pockets PU Leather Car Console Side Organizer-Orange,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer]

Car Seat Pockets PU Leather Car Console Side Organizer-Orange, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Oxalic Acid for 99.6% Purity, Part I.Kids Waterproof Softshell Jacket with Brushed Fleece Inside. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[API 594 Super Duplex Steel Single Disc Dual Disc Plate Wafer Check Valve]

CNC Turned Aluminum Anodized Auto Part, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),4-20mA PT100 Temperature Transmitter (PCT202).ASTM D2846 Schedule 40 CPVC 4-Ways Fittings Cross, 175-184[High Frequency Vacuum Wood Dryer Hfvd-14-CH]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes]

Whitaker,J.R.(1963),High Grade Nitrous Oxide Gas / Laughing Gas / N2o. Analytical Chemistry35(12), 1950-1953[Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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