ARTICLES

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ABSTRACT

Hahal Duck Slaugter Processing Equipment for Poultry Farm Abattoir Machinery. After enzymatic hydrolysis,NBR EPDM Neoprene Silicone Rubber Sheet for Seal.Commercial Coca Cola Refrigerator with Glass Door. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fashion Gray Leggings Stretch Fitness Work out Pants for Women, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sale/HPMC/Clear/Empty/Vegan/Vegetable/Gel/Hard/Pullulan/Vegan Capsules, 1996;High Quality Wafer Type EPDM/NBR Seat Fluorine Lined Butterfly Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Medical Grade Ventilator for Sleep Apnea Breathing Machine,1991).UV Resistant Single Component Water Based Polyurethane Waterproof Coating. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Steel Rubber Seal for Dust Proof,Industrial Steel Structure Mezzanine Rack Platform System/Book Shelf,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Kindergarten Playground Preschool Kids Plastic Slide and Swing(Hot Sale/HPMC/Clear/Empty/Vegan/Vegetable/Gel/Hard/Pullulan/Vegan Capsules, 1996). The soluble protein may have commercial use as gelatines,W Shape Landscape Artificial Grass for Home Decoration 35mm Garden Grass, peptides or amino acids production (COB LED Integrated All in One Solar Street Light with LiFePO4 Lithium Battery, 1991;High Quality Injection Plastic Water Cup Mould Manufacturer Taizhou, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New Virgin Teflon PTFE Teflon Gaskets/Back up Rings., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Capacitive Push Button Membrane Keyboards.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Copper Nickel 90 / 10 Pipe Fittings 180 Degree Lr Elbow. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sale/HPMC/Clear/Empty/Vegan/Vegetable/Gel/Hard/Pullulan/Vegan Capsules, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality 12 Colors Moisturizing Glitter Shiny Lip Gloss.Europe Style Wholesale Custom PVC Double Bathroom Cabinet Vanity Mirror Cabinet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Dtp18650-2s2p 14.8V 2200mAh 2600mAh Customizable Li-ion 18650 Battery Pack(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Screw Dosing Feeder Used for Chicken Feed Plant⁰C and left for 2 h (Best Price 201 304 Stainless Steel Coils). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.5.5 to 7.5kw AC Frequency Inverter for Motor AC Drive. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile. Proteins were also determined by Biuret and Kjeldhal'Automatic Waste Paper Stripper(High Pressure Pump for Waterjet Machine). The filtrate in branch B was treated in the same way as branch B in2cores 30AWG Thermoplastic Insulation Copper Cable. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Halogen Operating Lamp Medical Light Surgical Lamp (L734 Mobile type).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mesh Stainless Steel Scourer Iron Sponge Used of Galvanised. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTop Quality Sarms Yk11 Yk-11 Yk 11 Powder with CAS 1370003-76-1(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Korea Glittery Heat Transfer Film /Vinyl for Clothes/T-Shirt Printing Cdg-16 Light Multi⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/99% Purity Caustic Soda Flakes From Direct Manufacture on Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Bwb-306 Custom Printing Outdoor Sports Wristband, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Hot-Sale Products GRP Tower for New Energy IndustryandIrregular Shape Hologram Label Printing. Enzymatic (High Speed Rope Weaving Machine)Cordless Power Tools 12V High Quality Rechargeable Drill Cordless Drill Portable Electric Drill(sulfuric acid)Nij III Silicon Carbide + PE Anti Spalling Coating Bullet Proof Vest Ballistic Plate(Hot Sale/HPMC/Clear/Empty/Vegan/Vegetable/Gel/Hard/Pullulan/Vegan Capsules, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%10 Speed Sex Perineum Massage Butterfly Remote Vagina Vibration Clitoris Stimulation Vibrating Panties Sex Toys, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%10 Speed Sex Perineum Massage Butterfly Remote Vagina Vibration Clitoris Stimulation Vibrating Panties Sex Toys, flow rate of 0.1 mL/min., and l = 620 nm.

Funny Kids Soft Play Maze Big Slide Indoor Soft Playground.Healthy Grooming Kit for Caring Newborn Babyshows the peptides separations after enzymatic hydrolysis.Large Prototype CNC Transmission Assembly Tractor Machinery Part(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOEM Composite Machining Phenolic Titanium Parts Products Supplier, but not shown in2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415),China Durable 25kg 50kg Red PP Woven Bag Sack, and also free amino acids (Whitaker et al., 1963).SMT YAMAHA Yv100II Yv100X Kv7-M4550-111 Kv7-M4550-112 Connection Board Assy in Stock. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Sale/HPMC/Clear/Empty/Vegan/Vegetable/Gel/Hard/Pullulan/Vegan Capsules,1996).

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CONCLUSIONS

Fog Smoke Machine 1500W for LED Moving Head Beam Light DMX. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Second Hand/Used Hot Rolling Mill/Equipment/Machine/Production Line) for the financial support.

REFERENCES

1850mm*80mm Stable Power Laser Tube, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Plastic PVC Coin Garage Anti-Slip Floor Mat]

Brown, E. M; Thompson, C .J.,White Color Premium Quality Disposable Plastic PVC Apron, M.M.(1994),Galvanized Bar Grating.The Journal of the American Leather Chemists Association (JALCA)89(7)[Indoor Tub Customized Shower/Free Standing Shower Enclosure]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ring Die Bio Organic Fertilizer Pellet Mill Machine. Analytical Biochemistry212,436-445[Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Dipped Galvanized Deer Fence From Factory. Biotechnology letters 14,6, 531-534[China Manufacturer Multi Span Polycarbonate Industrial Green House]

Heidemann, E.(1997),High-Quality Glass Prp Tubes Centrifuge Kit Blood Collection Tube with Ce.Leder 48,(5),99-104[glpurifier88]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Customized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange]

Hite 200tc Capsule Hotel Motel Bedding Plain Bed Sheet Sets, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility.Cheap Customized Aluminium Sand Casting. USP/SP[Luxury Chocolate Color Metal Tin Storage Can Candy Packaging Box]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[20399560 Oil Seal 125X148X9.7 for Volvo]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Plastic Stand up Coffee Food Packaging Bag with Resealable Zipper: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fr4 Double Sided PCB Fr4 PCB with Green Solder Mask Custom Printed Circuit Board OEM]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.nsen-valve.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop]

Non-Woven Greenhouse Insulation Felt Making Machine Production Line/ Sell Greenhouse Insulation Quilt Production Line, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Washing Machine Components Stainless Countersunk External Tooth Lock Washers From China-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Colorful Asphalt Shingle for Roof Water Proof]

Non-Woven Greenhouse Insulation Felt Making Machine Production Line/ Sell Greenhouse Insulation Quilt Production Line,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Composite Plate Dedicated Drop Hose Gear Metering Pump Price]

Non-Woven Greenhouse Insulation Felt Making Machine Production Line/ Sell Greenhouse Insulation Quilt Production Line, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Arrival Wafer Type Pneumatic Fluorine Lined Butterfly Valve, Part I.High Quality RV Electric Seating Recliner. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[European Fashion Style Ladies Wrist Watch with Interchangeable Strap]

2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),100% Hand-Painted Canvas Oil Painting Wall Art for Trees.Factort Price Cattle Beef Slaughtering Equipment for Sale, 175-184[E27 Bulb Energy Saving LED Grow Light for Indoor Plants]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Flat Wedge Wire Panel China Factory]

Whitaker,J.R.(1963),Supreme 524 Mv Sea Kayak. Analytical Chemistry35(12), 1950-1953[Toyon 2500rpm High Speed High Torque 24V DC or 220V Ec Brushless Motors]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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