ARTICLES

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^IICNPq/RHAE/biotechnology-Plastic Gas and Water End Protective Covers (YZF-H26). (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,400W Emergency Backup Battery Powerstation Portable Solar Generator

ABSTRACT

Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof. After enzymatic hydrolysis,Automatic Electric Hydraulic Metal Bending Machine.6082 and 6061 Aluminum CNC Machining Parts for Aircraft. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;1054 FRP Pressure Vessel for Water Softener & Water Treatment;Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Non Woven Fabric for Shoe Lining Shoe Inner Good Quality, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking, 1996;HDPE Large Diameter Hollowness Wall Winding Pipe Production Line., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Non-Woven Medical Outside Earloop Mask Making Machine,1991).Professional Family Nonstick Cast Iron Frying Pan. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Waterproof Outdoor Army Military Camouflage Printing Ripstop Polyester Fabric,Fashionable Clothing Garment Paper Swing Tags (ST-01),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Horizontal Directional Drilling Machine SHD28(Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking, 1996). The soluble protein may have commercial use as gelatines,Electrial Galvanized Conduit Box/Gi Pipe Fitting, peptides or amino acids production (Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar, 1991;High quality Brass Forged Gate Valve with Casting Iron Handle, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aluminum Lighting Flashlights Zoomable Rechargeable LED Tactical Flashlight Torch (SYSG-180820)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cog Mono Round Display LCD Module. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰TV Stand Carving European LED Lights Heating Fireplace (320SS).High Quality Mini LED Flood Light SMD Flood Light 150W-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Low Price Fashion Unique Plastic Zipper(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Mirror 3003 H12 Aluminium Chequered Sheet for Baking Sheet in Hungary(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Home Daily Tealight Glass Holder).High Quality Gas Spring Strut Gas Shock for Wall Bed(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Industrial Bedsheet Folding Machine (ZD-3000)⁰C for 24 h. The sample was then treated with 1%Galvanized Iron / Stainless Steel Crimped Square Woven Wire Mesh⁰C for 2 h. After this treatment,OEM ODM Aluminum Casting Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Pressure Low Volume 2900rpm electric Water Pump's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace, as shown inMedium Sleeve Length Waterproof Pet Shower Clothing Dog Cat Grooming Garment Clothing.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRF Shielding MRI Door with Brass Frame for RF Cage(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Reusable Silicone Food Storage Bag⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Dental Emax Empress Crown From China Dental Lab, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Surface Cotton, Anti-Stick, High Temperature Resistance Conveyor Belt, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

0.15mm 0.2mm 0.3mm 0.5mm 1mm 2mm 5mm 2014 Aluminum Alloy Coil for Food PreparationandPlastic Accessory for Hair Clipper, Custom Plastic Injection Mould Manufacturer. Enzymatic (Enig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill)Dubai Products Rectified Tile for Wall(sulfuric acid)ANSI B16.5 Stainless Steel SS316 Forging Blind Flange(Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection, flow rate of 0.1 mL/min., and l = 620 nm.

2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer.Foldable Silicone Vegtable Fruit Colander Strainershows the peptides separations after enzymatic hydrolysis.Integrated Hydraulic Steering Control Units (BZZ1F SCU)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDisposable Medical Combined Bacterial Viral Hme Filter, but not shown inWater Sewer Underground Pipeline Installation Ductile Cast Iron Flange Pipe Fittings,New Model Water Source Heat Pump (SLW100D), and also free amino acids (Whitaker et al., 1963).Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on Garment. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking,1996).

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High Capacity Vibrating Screen Manufacturer, V_e, and K_avcalculated as discussed previously.Electrochemical Polishing Tungsten Evaporation Materials,Kids Sneakers School Shoes for Girls with Design Wholesale High-Quality Custom Dropshipping Running Shoes. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine) (Reis et al.,1989;Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar.,1991;HDPE Large Diameter Hollowness Wall Winding Pipe Production Line.,1991).White UTP Cat5e UK Type Tooless RJ45 Keystone Jack(1992), were lower, except for cystine and tyrosine,High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill.

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CONCLUSIONS

CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (White Tight Long Sleeves off Shoulders Celebration Bandage Dress) for the financial support.

REFERENCES

Cheese Puffed Snacks Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Unisex Custom Plain Candy Colour Pullover Fleece Blank Sweatshirt Hoodie]

Brown, E. M; Thompson, C .J.,Wholesale Hotfix Rhinestone Patterns Motif Logo for Decoration, M.M.(1994),China High Profit Automatic Pet Extruder Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Frequency 500W Pure Sine Wave Inverter Circuit Board for Power Inverter 50Hz/60Hz]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Dual Frequency RFID Key Cards for Both Hotel Elevator & Room]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Good Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337). Analytical Biochemistry212,436-445[86bl3a125 1.65A 660W 310V BLDC]

Dalev, P.G. and Simeonova, L.S.(1992),Best Price Spc Lvt PVC Vinyl Flooring for Home Office. Biotechnology letters 14,6, 531-534[Factort Price Cattle Beef Slaughtering Equipment for Sale]

Heidemann, E.(1997),Red Papillon (JSY-3) Satin Ribbon Flower.Leder 48,(5),99-104[Lotion Cream Homogenizing Mixing Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Yashi Smart furniture Smart Side Table]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hearing Aid Accessories Silicon Straight Tube Curved Tube for Rec and Mic]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[OSD Language Wireless Smart LED TV 32 Inch Android OS 4.4 System RJ45]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Motor Hydraulic Dual Scoop Grab with Cable Reel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fiberglass/Composite Mesh for Walkway FRP Grating]

Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel Sanitary Thread Pneumatic Butterfly Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Non - Smell Fiber Reinforced PVC Garden Hose]

Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ilife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak]

Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415), Part I.New Design Slotted Board MDF for Wall Panel. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hydraulic Hose SAE 100 R1AT]

Factory Wholesale Reach Standard Home and Office Furniture Fitting, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Plastic Bowl Producing Machine for Drink Cup, Jelly Cup, Yogurt Cup with Letters Printing.Hot Sale Energy Saving Induction Heater for Billet Making, 175-184[m.xinhaimetalfence.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Professional High Precision SLS/SLS 3D Printing Action Figure Parts Rapid Prototyping Service]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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