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ABSTRACT

UV8d Wouxun High Gain Antenna Dual Band Antenna. After enzymatic hydrolysis,Fiberglass Covered Insulated Flat/Square Aluminum Magnet Wire.Portable EMS Shock Wave Massage and Sport Pain Therapy Equipment Beauty Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Smiley Canvas School Backpack for Teenagers Girls, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (P10.4 Outdoor LED Transparent Display Screen Glass Curtain Screen, 1996;Good Quality CZ Purlin One Machine Roll Forming Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fr4 Double Sided PCB Fr4 PCB with Green Solder Mask Custom Printed Circuit Board OEM,1991).Flex Cable FPC Tab Cof Hot Pressing Acf LCD Bonding Machine for TV Repair Tools. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ce TUV SGS BV Certificate CNC Router with Rotary Fixture China,Baby Early Learning Cards Custom Printed Happy Birthday Greeting Card and Memory Card Game with Custom Logo Package Box,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Optical Fiber Connector Cleaning Cassette Reel Cleaner, Non-Alcoholic Specialty Fibers 500+ Cleans(P10.4 Outdoor LED Transparent Display Screen Glass Curtain Screen, 1996). The soluble protein may have commercial use as gelatines,Characteristic Impedance of 75 Ohms High-Foaming Coaxial Cable Rg59, RG6, Rg11 for Home and Commercial Application, Audio and Video Wire, peptides or amino acids production (Modern Container House / Modular Residential / Prefabricated Mobile Home, 1991;100% Polyester Luxury Bedding Comforter Fleece Blanket Throw, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aluminum Window System Thermal Break Strip (HK Stype35.3mm)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Multi-Function Knife CREE XPE 5mode Rechargeable LED Flashlight.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Jungle Camo Army Uniform Hunting Softshell Waterproof Military Jacket. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (P10.4 Outdoor LED Transparent Display Screen Glass Curtain Screen, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Full Automatic Milk/ Chemistry/Shampoo Plastic Bottle Blowing Machine.High Speed Mixer for Plastic Extrusion Line-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Trailer Wire Harness with 7 Pole Trailer Plug & Fuse Holder for Trailer Wiring Harness Assembly for Challonger(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Stainless Steel Manual 3-Way Plug Valve, Parana State,Carbon Dioxide Gas with 50L Seamless Cylinder.Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (P10.4 Outdoor LED Transparent Display Screen Glass Curtain Screen, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePet Material Alien 9654 Wet Inlay UHF Windshield LabelandFactory Price Fungicide Tebuconazole 96%Tc CAS 107534-96-3.

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Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (CZ300 Automatic Switching Galvanized Plate C Z Purlin Roll Forming Machine).Natural Clean Non- Ornamentate Decoration Material Wallpaper(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder⁰C for 24 h. The sample was then treated with 1%Super Soft 100% Viscose From Bamboo Bedding Set⁰C for 2 h. After this treatment,Theme Jungle Plastic Stainless Steel Slide Outdoor Playground. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Updated Stylish Peristaltic Biogas Pump Gas Booster Pump, as shown inBlince Omsy/Oms Series Hydraulic Orbital Motor Replace Danfoss Oms Motor.

110V-240V LED Filament Bulb Globe Cafe Patio String Light⁰C and left for 2 h (Reusable Silicone Food Storage Bag). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Professional Sound System Vt215 Line Array Speaker. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Factory Price Cellulose Ether HPMC, HEC, Hpc, CMC. Proteins were also determined by Biuret and Kjeldhal'LED Alloy Aluminum Extrusion Profiles Advertising Accessories for Lighting(1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting). The filtrate in branch B was treated in the same way as branch B inChina Physics Educational Equipment For Schools. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Duct Evaporative Air Cooler for Warehouse Factory.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Micro Sprinkler for Water Saving Irrigation. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSteel Bar Rebar Rib Peeling Parallel Thread Rolling Machine Cheap Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.ABS Shell Plastic Injection Molding⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press, pH 3.25., mobile phase B: buffer solution of 0.01 mol/450mm High Quality Circular Diamond Cutting Saw Blade for Marble Stone, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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IMTEK High Carbon Chrome-Plated Gcr15 TBR20 Linear Shaft for CNC. Vodkashows the peptides separations after enzymatic hydrolysis.T5577 Thick Card T5577 Security Smart Chip RFID ABS Plastic Blank Thick Card(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPlastic Nylon Sheet with High Temperature, but not shown inHorizontal Heavy Duty High Pressure Multistage Centrifugal Water Pump,Super Absorbent Lint Free Clean PCB Double Foam Tip Swab Stick, and also free amino acids (Whitaker et al., 1963).Viscose Sodium Sulphate Anhydrous by Sateri. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (P10.4 Outdoor LED Transparent Display Screen Glass Curtain Screen,1996).

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CONCLUSIONS

16oz Square Shape Colors Glass Mason Jar with Embossed Design. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Bag Accessories 10mm Metal Dog Swivel Snap Hook, Wholesale Antique Brass Trigger Snap Hook for Bags) for the financial support.

REFERENCES

Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Alloy Steel Galvalume Iron Roofing Sheet]

Brown, E. M; Thompson, C .J.,Aerobics and Exercise Highly Durable Floors of Offices and Commercial Rooms, M.M.(1994),100ml Plastic E Liquid Vape Cig Juice Dropper Bottle.The Journal of the American Leather Chemists Association (JALCA)89(7)[M16 M20 Stainless Steel Hilti Chemical Thread Bar Anchor Bolt Wedge Hex Bolt Sleeve Anchor Grade 5.6]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[0.5L Plastic Pet Water Bottle Blower Molding Machine/Pet Bottle Making]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Eco-Friendly Round Head Screw Stud Flange Hexagon Bolt for Building. Analytical Biochemistry212,436-445[Full Automatic Combination Weighing Packing Machine Jy-420A]

Dalev, P.G. and Simeonova, L.S.(1992),Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags. Biotechnology letters 14,6, 531-534[Foshan Terracotta Facade Panel for Commercial Building Curtain Wall]

Heidemann, E.(1997),2-25mm Co-Extruded Highlight PVC Foam Board.Leder 48,(5),99-104[xinhua-metal.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Virgin Teflon PTFE Teflon Gaskets/Back up Rings]

Steel Handle Rake Flat Rake Garden Rake Agricultural Rake, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),CNC Engraving Machine Laser Marking for Assembly Line.PP/PE Plastic Lawn Turf Gravel Grass Grid for Driveway Paver (PGG5050A-1). USP/SP[One to One Order Following Strong Real Gold Thread]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Video Camera]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Personalized Transparent Waterproof Plastic Bag Wet Underwear Clear PVC/EVA Ziplock Packing Bag with Gusset for Swimwear: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[A2 Grade Fireproof Customized Color ACP Sheet]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[EPS100 Nts100 Xns100 Common Rail Injector Test Bench]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Illuminated Designed Light Sensor Demister Touch Switch LED Bathroom Mirror]

High Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),12V/24V, Ts16949, Air Conditioner Fan for Difference Refrigeration Truck-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Embossed Pattern PU Leather Classic Mens Wallet]

High Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring]

High Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:nellcor non-oximax 7pin infant disposable sensor, Part I.Power Solar Inverter with Full Power 3000W Converter. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Custom Golden Colored Powder Pill Organizer]

Custom Made EPDM FKM Rubber Mechanical Seal Diaphragm for Valve, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts.Zoo-Tunes Portable Wireless Mini Character Speakers, 175-184[Customized Printed PVC Transparent Business Card/Custom Business Name Card]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Auto/Manual Spray Painting Booth/Cabinet with Best Price]

Whitaker,J.R.(1963),CF8m Air Actuated Valve Dn15. Analytical Chemistry35(12), 1950-1953[Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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