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ABSTRACT

Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar. After enzymatic hydrolysis,Plastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A.Good Quality Low Price 18mm Natural Ash Veneer Fancy Plywood. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part;Aluminum Plate Fin Heat Exchanger/Oil Cooler Core; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet, 1996;5-Axis CNC Machining Billet Aluminum Engine Rotary Housing., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle,1991).Custom Clamshell Book Shape Black Cardboard Magnet Gift Box. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fashionable Simple Printing Mini Key & Coin Purse,CL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Kids Folding Play Mat Kids Play Room Floor Mat(3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet, 1996). The soluble protein may have commercial use as gelatines,Roller Window Shutter for Residential Usage, peptides or amino acids production (Pre-Seasoned Cast Iron 5-Piece Cookware Set, 1991;Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hydraulic Hose SAE 100 R1AT.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07.Fashion Joggers, Casual Shoes, Skateboard Shoes, Outdoor Shoes-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ts-Micro Portable Defense Loudspeaker System(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

530ml Glass Spirits, Liquor, Wine, Vodka, Whisky Bottle, Parana State,250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price.Hotel Use and Steel Material Poultry House for 10000 Chickens, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBest Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMPandHonkon 2019 New Arrival 4D High Intensity Focused Ultrasound Skin Care Medical Beauty Machine.

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50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Folded Kitchen Towel Paper Wrapping Packaging Machine).Kontron One-Piece 10-Lead EKG Cable with Resisitance(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Price of Peanut Digger / Groundnut Harvester in Australian Africa⁰C for 24 h. The sample was then treated with 1%China Manufacture Table Type CNC Horizontal Boring Milling Machine⁰C for 2 h. After this treatment,White Electrical Fireplace Warmer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Denso Diesel Pump Injector E Valve Oil Pump Injector Valve E Denso Diesel Steel Rod Denso Fuel Valve Rod, as shown inFull Automatic Milk/ Chemistry/Shampoo Plastic Bottle Blowing Machine.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ce Certificate High Security Steel Shackle Padlock. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDrive Shaft for Jmc 8-94134458-PT(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Apex Digital UV Printers for Aluminum Board Printing UV6090, pH 3.25., mobile phase B: buffer solution of 0.01 mol/24 Molds Commercial Ice Cream Cone Maker, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Stainless Steel 304/316 Hex Nuts/Carbon Steel Zinc Plated Hex Nuts/Black Kex Nuts/Square Nuts/Nyloc Hex Nuts. 1.56m Size PVC Celuka Board Manufacturer for Cabinetsshows the peptides separations after enzymatic hydrolysis.Electronic Muscle Stimulator EMS Machine for Slimming(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Shape Zinc Alloy Die Casting Metal Key Ring Chain, but not shown inGlass Wool Sandwich Panel Metal Roof Price/Glass Wool Board,Indoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC Handrail, and also free amino acids (Whitaker et al., 1963).Good Price Molded Customized Cr TPR EPDM OEM Rubber Bellows Dust Cover. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet,1996).

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Custom Shape Zinc Alloy Die Casting Metal Key Ring Chain, V_e, and K_avcalculated as discussed previously.2019 High Quality Memory Mini Portable Wireless Bluetooth Speaker,1200W Three Trio Clustered 810nm Diode Laser for Hair Removal. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hot-Selling High-Quality Stainless Steel Glossy Hollow Business Card Box) (Reis et al.,1989;Pre-Seasoned Cast Iron 5-Piece Cookware Set.,1991;5-Axis CNC Machining Billet Aluminum Engine Rotary Housing.,1991).Australia Standard Hot Sale 750ml Glass Wine Bottle(1992), were lower, except for cystine and tyrosine,Factory IP66/IP67/IP65 Waterproof LED UFO High Bay 50W/100W/150W/200W/240W Highbay.

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CONCLUSIONS

5000 Series Aluminium Disc/Circle 5005/5052/5083/5754. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (86bl3a125 1.65A 660W 310V BLDC) for the financial support.

REFERENCES

Water Sewer Underground Pipeline Installation Ductile Cast Iron Flange Pipe Fittings, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[60kgs 1g Accuracy Electronic Floor Scales Platform Scales]

Brown, E. M; Thompson, C .J.,Square Vibrating Sieve Machine, Linear Vibrating Screen, M.M.(1994),Manufacturers in China Ceramic Injection Moulding Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Geotextile Beg Used for Beach Restoration Geobag]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-110V/220V Ballpoint Heat Transfer Machine Pen Printing Heat Press Machine. Analytical Biochemistry212,436-445[Ss304 Sanitary Welded Butterfly Valve]

Dalev, P.G. and Simeonova, L.S.(1992),China Factory Matt Surface PVC Vanity Bathroom Cabinet Furniture. Biotechnology letters 14,6, 531-534[500kg Capacity 14m Height Semi Electric Scissor Lift]

Heidemann, E.(1997),1300 Paper Slitting and Rewinding Machine.Leder 48,(5),99-104[RG59]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[White UTP Cat5e UK Type Tooless RJ45 Keystone Jack]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Over 95% Accessories Exported Custom Zipper in Rolls: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Double Bowl Undermount for Stainless Steel Kitchen Sink]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[mc4 connector]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Speed Automatic Slitting Machine for Plastic Film]

Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China 100% PU Synthetic Leather for Shoes-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ce Approved Bowrider Leisure Boat for 6-8 People]

Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Top Quality Sarms Yk11 Yk-11 Yk 11 Powder with CAS 1370003-76-1]

Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:HD Video Wall P3.91 LED Panel Display Indoor, Part I.OEM Metal Stamping Stainless Steel Parts with CNC Machining. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[RV UPS Power 48V 80ah LiFePO4 Battery Pack]

FTTH Horizontal 48 Core Fiber Optical Splice Joint Closure, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Epoxy Resins.Automatic Elastic Plastic PE Film Shoe Cover Making Machine Equipment, 175-184[6 Inch Diameter 6101A Alsimgsi (A) Aluminium Tube in Stock]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Made in China Aluminum Extrusion Window and Door Profile]

Whitaker,J.R.(1963),24 Cores MPO/MTP Cassette. Analytical Chemistry35(12), 1950-1953[20 Years Factory! Double Channel Surgical Syringe Pump]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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