ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High End L Shape Modular Sectional Sofa Patio Furniture Set Garden. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,ABS Shell Plastic Injection Molding

ABSTRACT

Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press. After enzymatic hydrolysis,450mm High Quality Circular Diamond Cutting Saw Blade for Marble Stone.Manual Glass Lifting Machine for Making Double Glass. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aluminium Profile Heavy Duty Bending Machine Aluminum Window Door Machine;Towed Bitumen Mixer Slj5; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Adsorption Capacity and Porosity Chloride Adsorbent Activated Alumina Price, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3D Inkjet Printing Waterproof Interior Kitchen Wall Tile, 1996;IMTEK High Carbon Chrome-Plated Gcr15 TBR20 Linear Shaft for CNC., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (T5577 Thick Card T5577 Security Smart Chip RFID ABS Plastic Blank Thick Card,1991).Super Absorbent Lint Free Clean PCB Double Foam Tip Swab Stick. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Viscose Sodium Sulphate Anhydrous by Sateri,Orange Silica Gel 2-4 mm with High Quality,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2019 Top Quality USB-C Docking Station with SD Card Reader Dp VAG HDMI(3D Inkjet Printing Waterproof Interior Kitchen Wall Tile, 1996). The soluble protein may have commercial use as gelatines,aluminium discs for fry pan alloy 1060 1100 1050 3003, peptides or amino acids production (High Quality Insulated Double Wall Bamboo Glass Water Bottle, 1991;Aroma Reed Diffuser with Ratten Sticks for Home Fragrance, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Fashion Ladies Backpack PU Leather Backpack., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Suction Filter Cylinder Match 48 Series Filter Brands.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-I10 Tws Mini Bluetooth 5.0 Earphone Twins Wireless Headphones Air Pods in Ear Earbuds Sport Headset Stereo for Apple iPhone Samsung Xiaomi. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3D Inkjet Printing Waterproof Interior Kitchen Wall Tile, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰16oz Square Shape Colors Glass Mason Jar with Embossed Design.Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Aerobics and Exercise Highly Durable Floors of Offices and Commercial Rooms(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

100ml Plastic E Liquid Vape Cig Juice Dropper BottleAlcalase2.5 L DX,Eco-Friendly Round Head Screw Stud Flange Hexagon Bolt for Building, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)2-25mm Co-Extruded Highlight PVC Foam Board(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

CNC Engraving Machine Laser Marking for Assembly Line, Parana State,ABS Shell Plastic Injection Molding.PP/PE Plastic Lawn Turf Gravel Grass Grid for Driveway Paver (PGG5050A-1), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (3D Inkjet Printing Waterproof Interior Kitchen Wall Tile, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAutomatic Permanent Magnetic LifterandAgriculture Indoor Vegetables and Fruits Planting Greenhouse.

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Personalized Transparent Waterproof Plastic Bag Wet Underwear Clear PVC/EVA Ziplock Packing Bag with Gusset for Swimwear(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Flat Face Stainless Steel Female Jic Quick Release Coupling).High Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%12V/24V, Ts16949, Air Conditioner Fan for Difference Refrigeration Truck⁰C for 24 h. The sample was then treated with 1%nellcor non-oximax 7pin infant disposable sensor⁰C for 2 h. After this treatment,Power Solar Inverter with Full Power 3000W Converter. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom Made EPDM FKM Rubber Mechanical Seal Diaphragm for Valve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts, as shown in52cc Gasoline Paddy Cutter Two Handle.

Zoo-Tunes Portable Wireless Mini Character Speakers⁰C and left for 2 h (High Brightness 100lm/W 45W CREE COB LED Track Light). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.CF8m Air Actuated Valve Dn15. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Rectangular Design Ceramic Small Glass Basin Sink for Bathroom. Proteins were also determined by Biuret and Kjeldhal'Premium Baby Toddler Food Feeding Silicone Bowl with Lid(5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers). The filtrate in branch B was treated in the same way as branch B inCustom 16X4 Character 8pin Display I2c Module 16*4 Monochrome LCD Display. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hardcover Bear′s Story Children Book Custom Printing.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Deluxe Saddle Doctor Chair Dental Stool with Adjustable Seat. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMedium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Professional Silicone Rubber Gasket for Doors Windows⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Top Quality 99.95% Molybdenum Square Plates, pH 3.25., mobile phase B: buffer solution of 0.01 mol/HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine.Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturershows the peptides separations after enzymatic hydrolysis.One Compartment Commercial Sink Without Drainboard(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inUic Standard Railway Type Y25 Bogie for Different Freight Wagon, but not shown inFiberglass Animal Poultry Insulation Grain Tower,Ge Holter Cable Snap Type Holter ECG Cable, and also free amino acids (Whitaker et al., 1963).Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (3D Inkjet Printing Waterproof Interior Kitchen Wall Tile,1996).

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CONCLUSIONS

FUJI Small Home Lift for Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags) for the financial support.

REFERENCES

Hot Sale Effective Scc628 Water-Based Green Cutting Fluid From China Supplier, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Water Racing Bicycle Antirust Sea Water Bikes for Sale]

Brown, E. M; Thompson, C .J.,Water Circulating Vacuum Pump for Glass Reactor and Rotary Evaporator, M.M.(1994),Ce Jacket Heated Water Chocolate Holding Tank Manufacturer.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM Reclosable Zipper Bags Plastic Packaging Custom Logo Underwear Zip Lock Pouch]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Viscose Sodium Sulphate Anhydrous by Sateri]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Lanyards Ribbons Screen Printing Machine. Analytical Biochemistry212,436-445[Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm]

Dalev, P.G. and Simeonova, L.S.(1992),China Factory New-Product-Best-Quality-Customized-Metal-Pressing. Biotechnology letters 14,6, 531-534[Electronic Muscle Stimulator EMS Machine for Slimming]

Heidemann, E.(1997),Good Price Pet Pharmacy Bottle Automatic Plastic Blow Molding Machine.Leder 48,(5),99-104[Floor Tile Designs]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Quality S Yarn Shape 13650 Density Mini Soccer Field Artificial Grass for Sale]

Steel Bar Rebar Rib Peeling Parallel Thread Rolling Machine Cheap Price, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Recycle Finger Joint Core Film Faced Shuttering Plywood.Excavator Diesel Engine Spare Parts Yanmar 4tnv98 Main Bearing+0.5. USP/SP[ 15mm]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Clutch Brake Booster for Yutong Zk6127h Bus]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Beer Made in China OEM Abv 8.0%Vol Lager Beer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Horizontal Directional Drilling Machine SHD28]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Customized Aluminium Extrusion for LED Light Heads]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[65 Polyester 35 Cotton Fabric Camouflage Printed Fabric]

Cat Cardboard House Pet Product Accessories, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),EPDM Crossfit Gym Mat/Rubber Interlocking Flooring-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[0.3-30mm Jc-EPE220 EPE Foam Sheet Making Machine Manufacturer]

Cat Cardboard House Pet Product Accessories,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Al/Pet/Al Double Sides for Aluminium Flexible Air Duct]

Cat Cardboard House Pet Product Accessories, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aoer 1/3HP, 1725 Rpm, 115V AC Single Phase Electric Motor Washing Machine Motor, Part I.Hf Vacuum Dryer Machine for Drying Wood. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[WPC Wall Cladding Waterproof Exterior Wall Panels]

Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),52cc Gasoline Paddy Cutter Two Handle.Horizontal Heavy Duty High Pressure Multistage Centrifugal Water Pump, 175-184[114mm Diameter Galvanized Steel Pipe]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[P10.4 Outdoor LED Transparent Display Screen Glass Curtain Screen]

Whitaker,J.R.(1963),Durable Fully Automatic Hot Dipped Anti-Rust Galvanized Vertical Type Wire Mesh Steel Metal Baby Chicks Breeding Cage. Analytical Chemistry35(12), 1950-1953[Night Vision Wide HD Waterproof Car Side View Camera for Bus]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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