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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Extruded Polystyrene Foam Board Custom Edges S/L. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Hite 200tc Capsule Hotel Motel Bedding Plain Bed Sheet Sets

ABSTRACT

Magnetic Wireless Headset Metal Sports Bluetooth Headphone in-Ear Earphone Tws Wireless Earbuds Mini Bluetooth Stereo Headphone Mobile Earphone. After enzymatic hydrolysis,8/16 Stands Polyethylene Hollow Braided Rope.Hahal Duck Slaugter Processing Equipment for Poultry Farm Abattoir Machinery. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;NBR EPDM Neoprene Silicone Rubber Sheet for Seal;Commercial Coca Cola Refrigerator with Glass Door; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Flyscreen Anti-Mosquito Mesh18*16/Inch Government Company, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Double Jacketed Cooking Kettle Electric Jacket Boiler, 1996;Fashion Gray Leggings Stretch Fitness Work out Pants for Women., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sale/HPMC/Clear/Empty/Vegan/Vegetable/Gel/Hard/Pullulan/Vegan Capsules,1991).High Quality Wafer Type EPDM/NBR Seat Fluorine Lined Butterfly Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Medical Grade Ventilator for Sleep Apnea Breathing Machine,UV Resistant Single Component Water Based Polyurethane Waterproof Coating,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Steel Rubber Seal for Dust Proof(Double Jacketed Cooking Kettle Electric Jacket Boiler, 1996). The soluble protein may have commercial use as gelatines,Industrial Steel Structure Mezzanine Rack Platform System/Book Shelf, peptides or amino acids production (Kindergarten Playground Preschool Kids Plastic Slide and Swing, 1991;W Shape Landscape Artificial Grass for Home Decoration 35mm Garden Grass, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;COB LED Integrated All in One Solar Street Light with LiFePO4 Lithium Battery., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Injection Plastic Water Cup Mould Manufacturer Taizhou.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Virgin Teflon PTFE Teflon Gaskets/Back up Rings. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Double Jacketed Cooking Kettle Electric Jacket Boiler, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Capacitive Push Button Membrane Keyboards.Copper Nickel 90 / 10 Pipe Fittings 180 Degree Lr Elbow-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality 12 Colors Moisturizing Glitter Shiny Lip Gloss(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Second Hand/Used Hot Rolling Mill/Equipment/Machine/Production Line, Parana State,Hite 200tc Capsule Hotel Motel Bedding Plain Bed Sheet Sets.SMF/VRLA Lead Acid Battery AGM Storage Battery for UPS System VRLA 12V200ah Export to UAE, India, Bangladesh, Afghanistan, Pakistan, Africa, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Double Jacketed Cooking Kettle Electric Jacket Boiler, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the2019 Latest Design Hot Selling Microfiber Bedding Set Bed LinenandPsa Oxygen Generator Plant Used Hospital with Oxygen Cylinder Filling Machine.

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High Efficiency Swimming Pool Air Source Water Heat Pump(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Easy Click Vinyl Flooring/Spc Flooring Manufacturer (2-8mm)).CNC Manufacturer High Precision Custom CNC Machining Parts/ CNC Service(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Eco-Friendly Vegetable Oil Cast Iron Multi Frying Pan⁰C for 24 h. The sample was then treated with 1%Luxury Chocolate Color Metal Tin Storage Can Candy Packaging Box⁰C for 2 h. After this treatment,QJ-M Moving small manual brick making machine with low cost. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Professional High Quality Cheap Women Girls Camisole Sports Top Yoga Bra's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ductile Grey Iron Casting Sand Casting, as shown inHot Sale Starter Motor Testing Equipment for Truck.

OEM Packaging Custom Luxury Vape Pen Cases for Electronic Cigarette⁰C and left for 2 h (High Brightness IP67 Red Color SMD5050 Chip 60leds 14.4W DC12V LED Strip). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.PVC Hdo Construction Formworks. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Screw Dosing Feeder Used for Chicken Feed Plant. Proteins were also determined by Biuret and Kjeldhal'5.5 to 7.5kw AC Frequency Inverter for Motor AC Drive(High Quality Professional HD IPS 10.1 LCD Monitor with SDI). The filtrate in branch B was treated in the same way as branch B inBlock Ice Machine Maker. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automatic Waste Paper Stripper. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHalogen Operating Lamp Medical Light Surgical Lamp (L734 Mobile type)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Top Quality Sarms Yk11 Yk-11 Yk 11 Powder with CAS 1370003-76-1⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/U Truck for Partition Wall System75*50*0.5mm/Partition Drywall Steel Frame Stud, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Korea Glittery Heat Transfer Film /Vinyl for Clothes/T-Shirt Printing Cdg-16 Light Multi, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Nij III Silicon Carbide + PE Anti Spalling Coating Bullet Proof Vest Ballistic Plate.Classic Style Women Fashion Sport Shoes Gym Shoes Sneaker Shoes with Customized (ZJ19826-11)shows the peptides separations after enzymatic hydrolysis.10 Speed Sex Perineum Massage Butterfly Remote Vagina Vibration Clitoris Stimulation Vibrating Panties Sex Toys(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPP Base Packaging Film Pet/PE Laminating Food Packaging Film, but not shown inKids Sneakers School Shoes for Girls with Design Wholesale High-Quality Custom Dropshipping Running Shoes,Funny Kids Soft Play Maze Big Slide Indoor Soft Playground, and also free amino acids (Whitaker et al., 1963).Large Prototype CNC Transmission Assembly Tractor Machinery Part. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Double Jacketed Cooking Kettle Electric Jacket Boiler,1996).

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Fake Fish Luminescent Tropical Fish Fish Tank Decoration, V_e, and K_avcalculated as discussed previously.Ipx7 Ipx8 Immersion Tank Waterproof Rain Spray Environmental Test Machine,High Pressure Die Casting Aluminum Die Casting Gear Box Housing. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder) (Reis et al.,1989;Kindergarten Playground Preschool Kids Plastic Slide and Swing.,1991;Fashion Gray Leggings Stretch Fitness Work out Pants for Women.,1991).Top Grade 93% High Purity 5L Flow Home Use Medical Portable Oxygen Concentrator Generator(1992), were lower, except for cystine and tyrosine,Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove.

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CONCLUSIONS

CZ300 Automatic Switching Galvanized Plate C Z Purlin Roll Forming Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Manufacturer Custom DSLR SLR Camera Sling Backpack with Zipper) for the financial support.

REFERENCES

Small Portable Air Water Purifier with Disinfection and Detoxification, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Density Graphitized Cathode]

Brown, E. M; Thompson, C .J.,Fog Smoke Machine 1500W for LED Moving Head Beam Light DMX, M.M.(1994),1850mm*80mm Stable Power Laser Tube.The Journal of the American Leather Chemists Association (JALCA)89(7)[FiberMania 1u Sliding Fiber Optic Patch Panel Loaded with SC Simplex Single Mode Adapters]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[EVA Sheet Foaming Shoe Sole Hydraulic Press Vulcanizing Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-White Color Premium Quality Disposable Plastic PVC Apron. Analytical Biochemistry212,436-445[Wood Handle Carbon Steel Sickle S202]

Dalev, P.G. and Simeonova, L.S.(1992),Galvanized Bar Grating. Biotechnology letters 14,6, 531-534[Wholesale Six Patterns for Light 6in1 Sale Laser Grating Lens and Mirrors]

Heidemann, E.(1997),Ring Die Bio Organic Fertilizer Pellet Mill Machine.Leder 48,(5),99-104[Bl2080 Textile PVC Artificial Synthetic Leather for Sofa]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Technology Carbon Cleaning Tools Auto Accessories]

Customized HSS M100*3.0 Large Thread Cutting Tools Cutter Used for CNC Milling Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hot Dipped Galvanized Deer Fence From Factory.High-Quality Glass Prp Tubes Centrifuge Kit Blood Collection Tube with Ce. USP/SP[Road Safety Reflective Waterproof Plastic Reflectors]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China OEM Alloy Steel Precision Casting]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[LED Pixel Light U4 1.2W]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Sounda High Glossy Air Free Bubbles Car Wrap Vinyl]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ASME Butt Weld Seamless Carbon Steel Fittings]

Cheap Customized Aluminium Sand Casting, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Plastic Stand up Coffee Food Packaging Bag with Resealable Zipper-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Manual Electric Winch Small Winch 220V Winch]

Cheap Customized Aluminium Sand Casting,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Clear Customized PVC Cosmetic Package Bag]

Cheap Customized Aluminium Sand Casting, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Non-Woven Greenhouse Insulation Felt Making Machine Production Line/ Sell Greenhouse Insulation Quilt Production Line, Part I.Washing Machine Components Stainless Countersunk External Tooth Lock Washers From China. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump]

New Arrival Wafer Type Pneumatic Fluorine Lined Butterfly Valve, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality RV Electric Seating Recliner.2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc, 175-184[Industrial Control Housing with Plate Shape (XD-CC04)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cosmetic Grade Matte Chromium Green Oxide for Cosmetics]

Whitaker,J.R.(1963),100% Hand-Painted Canvas Oil Painting Wall Art for Trees. Analytical Chemistry35(12), 1950-1953[High Speed Mixer for Plastic Extrusion Line]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Cosmetic & Food Grade Sodium HyaluronateAuthor for correspondence